PERMANENT GENETIC RESOURCES: Identification of nuclear microsatellite loci for <i>Ipomopsis aggregata</i> and the distribution of pairwise relatedness in a natural population

Stearns, Frank W.; Boles, Sandra B.; Hurston, Heather; Vo, Tanya; Butler, D.; Shuham, Walter; Juenger, Thomas E.

doi:10.1111/j.1471-8286.2007.01984.x

Cited by 3 publications

(3 citation statements)

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“…The 13 microsatellite primer pairs described here are an important addition to the limited number of genetic markers currently available for Ipomopsis (Wu, 2006; Stearns et al, 2008), an emerging system for studying the dynamics of ecological speciation in flowering plants. These polymorphic markers should also be useful for population genetic studies within the Polemoniaceae, as evidenced by their amplification success in four congeneric and four more distantly related species.…”

Section: Discussionmentioning

confidence: 99%

“…Previous work in one such hybrid zone suggests that the contemporary genetic structure has been influenced by a combination of pollinator and habitat‐mediated selection on hybrids and the parental species (reviewed in Campbell, 2005), but elucidating the fine‐scale genetic composition of the hybrid populations has proven difficult (Wu and Campbell, 2005). These efforts have largely been challenged by a paucity of genetic markers needed for population genetic studies (Wu, 2006; Stearns et al, 2008). Here, we developed new microsatellite markers for I. aggregata to use to explore the genetic structure of natural Ipomopsis hybrid populations and in further molecular analyses of the Polemoniaceae.…”

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confidence: 99%

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Microsatellite loci in Ipomopsis aggregata (Polemoniaceae) and cross‐species applicability for ecological genetics studies

Smith

Massaro

2012

American J of Botany

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Microsatellite loci in Ipomopsis aggregata (Polemoniaceae) and cross‐species applicability for ecological genetics studies

Smith

Massaro

2012

American J of Botany

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“…Genomic DNA was extracted from leaf tissue using a DNeasy Plant tissue kit (Qiagen). Genomic DNA was genotyped at 11 nuclear microsatellite loci derived from four custom enrichment microsatellite libraries (Stearns et al 2008). This set of microsatellites covers six of seven I. aggregata chromosomes, as determined by standard linkage analysis (Kenney 2011).…”

Section: Neutral Genetic Markersmentioning

confidence: 99%

Adaptive differentiation in floral traits in the presence of high gene flow in scarlet gilia (Ipomopsis aggregata)

2016

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Plant-pollinator interactions are thought to be major drivers of floral trait diversity. However, the relative importance of divergent pollinator-mediated selection vs. neutral processes in floral character evolution has rarely been explored. We tested for adaptive floral trait evolution by comparing differentiation at neutral genetic loci to differentiation at quantitative floral traits in a putative Ipomopsis aggregata hybrid zone. Typical I. aggregata subsp. candida displays slender white tubular flowers that are typical of flowers pollinated by hawkmoths, and subsp. collina displays robust red tubular flowers typical of flowers pollinated by hummingbirds; yet, hybrid flower morphs are abundant across the East Slope of the Colorado Rockies. We estimated genetic differentiation (F ) for nuclear and chloroplast microsatellite loci and used a half-sib design to calculate quantitative trait divergence (Q ) from collection sites across the morphological hybrid zone. We found little evidence for population structure and estimated mean F to be 0.032. Q values for several floral traits including corolla tube length and width, colour, and nectar volume were large and significantly greater than mean F . We performed multivariate comparisons of neutral loci to genetic correlations within and between populations and found a strong signal for divergent selection, suggesting that specific combinations of floral display and reward traits may be the targets of selection. Our results show little support for historical subspecies categories, yet floral traits are more diverged than expected due to drift alone. Non-neutral divergence for multivariate quantitative traits suggests that selection by pollinators is maintaining a correlation between display and reward traits.

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Genetic Mapping Reveals an Anthocyanin Biosynthesis Pathway Gene Potentially Influencing Evolutionary Divergence between Two Subspecies of Scarlet Gilia (Ipomopsis aggregata)

Campitelli

Kenney

Hopkins

et al. 2017

Molecular Biology and Evolution

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Immense floral trait variation has likely arisen as an adaptation to attract pollinators. Different pollinator syndromes-suites of floral traits that attract specific pollinator functional groups-are repeatedly observed across closely related taxa or divergent populations. The observation of these trait syndromes suggests that pollinators use floral cues to signal the underlying nectar reward, and that complex trait combinations may persist and evolve through genetic correlations. Here, we explore pollinator preferences and the genetic architecture of floral divergence using an extensive genetic mapping study in the hybrid zone of two Ipomopsis aggregata subspecies that exhibit a hummingbird and a hawkmoth pollinator syndrome. We found that natural selection acts on several floral traits, and that hummingbirds and hawkmoths exhibited flower color preferences as predicted by their respective pollinator syndromes. Our quantitative trait loci (QTL) analyses revealed 46 loci affecting floral features, many of which colocalize across the genome. Two of these QTL have large effects explaining >15% of the phenotypic variance. The strongest QTL was associated with flower color and localized to a SNP in the anthocyanin biosynthesis pathway gene, dihydroflavonol-4-reductase (DFR). Further analysis revealed strong associations between DFR SNP variants, gene expression, and flower color across populations from the hybrid zone. Hence, DFR may be a target of pollinator-mediated selection in the hybrid zone of these two subspecies. Together, our findings suggest that hummingbirds and hawkmoths exhibit contrasting flower color preferences, which may drive the divergence of several floral traits through correlated trait evolution.

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PERMANENT GENETIC RESOURCES: Identification of nuclear microsatellite loci for Ipomopsis aggregata and the distribution of pairwise relatedness in a natural population

Cited by 3 publications

References 9 publications

Microsatellite loci in Ipomopsis aggregata (Polemoniaceae) and cross‐species applicability for ecological genetics studies

Microsatellite loci in Ipomopsis aggregata (Polemoniaceae) and cross‐species applicability for ecological genetics studies

Adaptive differentiation in floral traits in the presence of high gene flow in scarlet gilia (Ipomopsis aggregata)

Genetic Mapping Reveals an Anthocyanin Biosynthesis Pathway Gene Potentially Influencing Evolutionary Divergence between Two Subspecies of Scarlet Gilia (Ipomopsis aggregata)

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