Permanent Genetic Resources added to Molecular Ecology Resources Database 1 August 2009–30 September 2009

Abstract

This article documents the addition of 238 microsatellite marker loci and 72 pairs of Single Nucleotide Polymorphism (SNP) sequencing primers to the Molecular Ecology Resources Database. Loci were developed for the following species: Adelges tsugae, Artemisia tridentata, Astroides calycularis, Azorella selago, Botryllus schlosseri, Botrylloides violaceus, Cardiocrinum cordatum var. glehnii, Campylopterus curvipennis, Colocasia esculenta, Cynomys ludovicianus, Cynomys leucurus, Cynomys gunnisoni, Epinephelus coioides, Eunicella singularis, Gammarus pulex, Homoeosoma nebulella, Hyla squirella, Lateolabrax japonicus, Mastomys erythroleucus, Pararge aegeria, Pardosa sierra, Phoenicopterus ruber ruber and Silene latifolia. These loci were cross-tested on the following species: Adelges abietis, Adelges cooleyi, Adelges piceae, Pineus pini, Pineus strobi, Tubastrea micrantha, three other Tubastrea species, Botrylloides fuscus, Botrylloides simodensis, Campylopterus hemileucurus, Campylopterus rufus, Campylopterus largipennis, Campylopterus villaviscensio, Phaethornis longuemareus, Florisuga mellivora, Lampornis amethystinus, Amazilia cyanocephala, Archilochus colubris, Epinephelus lanceolatus, Epinephelus fuscoguttatus, Symbiodinium temperate-A clade, Gammarus fossarum, Gammarus roeselii, Dikerogammarus villosus and Limnomysis benedeni. This article also documents the addition of 72 sequencing primer pairs and 52 allele specific primers for Neophocaena phocaenoides.

Contrasted levels of genetic diversity in a benthic Mediterranean octocoral: Consequences of different demographic histories?

“…All individuals were genotyped at seven microsatellite loci: C21, C30, C40, S14 (Molecular Ecology Resources Primer Development Consortium et al., 2010), Ever007, Ever009 (Holland, Dawson, Horsburgh, Krupa, & Stevens, 2013), Mic56 (This study). All loci were amplified according to the PCR protocols described in Appendix S1.…”

Determinants of Genetic Structure in a Nonequilibrium Metapopulation of the Plant Silene latifolia

“…The observed levels of population genetic structure are also consistent with other published datasets on plant metapopulations using microsatellite markers, including other Silene species. Previous research has shown that the sub-structuring of alleles is not the consequence of PCR artifacts such as null alleles [34]–[36]. We estimated a significantly negative Pearson product-moment correlation coefficient between the genetic diversity of individual populations and that population's contribution to F ST (r = −0.3601, 95% CI −0.6260, −0.0191; P = 0.0396; Table S1).…”

“…Our lowest F ST corresponded to the only marker composed of a dinucleotide repeat. 1 [34], 2 [35], 3 [36].…”

“…However, previous studies have indicated the potential for metapopulation structure to have a significant outcome on selective dynamics and vice versa [61]. Since we derived our molecular markers from non-coding regions, they were assumed to be neutral and to be unlinked to functionally important genomic regions [34]–[36]. Although our panel of markers does not exhibit discontinuities in their distribution characteristic of outliers, given our limited panel size and the complexity of detecting outliers in hierarchically structured populations [62], we have only limited power to test this assumption.…”

“…We genotyped each plant at 8 microsatellite loci. Microsatellites were derived from multiple sources [34]–[36]. PCR amplification was conducted using previously published methods [37].…”