Permanent Genetic Resources added to Molecular Ecology Resources Database 1 June 2010 – 31 July 2010

Andris, Malvina; Aradottir, G. I.; Arnau, Gemma; Audzijonytė, Asta; Bess, Emilie; Bonadonna, Francesco; BOURDEL, G.; Bried, Joël; Bugbee, Gregory J.; Burger, Pamela A.; Chaïr, Hâna; Charruau, Pauline; Ciampi, A. Y.; Costet, Laurent; DeBarro, Paul J.; Delatte, Hélène; Dubois, Marie‐Pierre; Eldridge, Mark D. B.; England, Phillip R.; Enkhbileg, Dulamtseren; Fartek, Benjamin; Gardner, Michael G.; Gray, Karen-Ann; Gunasekera, Rasanthi M.; HANLEY, STEVEN J.; HAVIL, NATHAN; Hereward, James; Hirase, Shotaro; Hong, Yan; Jarne, Philippe; Jianfei, Qi; Johnson, Rebecca N.; Kanno, Manami; Kijima, Akihiro; KIM, HYUN C.; KIM, KWAN S.; KIM, WOO‐JIN; LaRue, Elizabeth A.; LEE, JANG W.; LEE, JEONG‐HO; LI, CHUNHONG; Liao, Ming-Hui; Lo, Nathan; Lowe, A. J.; Malausa, Thibaut; Malé, Pierre‐Jean G.; Marko, Michelle D.; Martin, Jean‐François; Messing, Russell H.; Miller, Karen J.; MIN, BYEONG‐WHA; Myeong, Jeong‐In; Nibouche, Samuel; Noack, Ann E.; Noh, Jae Koo; Orivel, Jérôme; PARK, CHOUL‐JI; Pétro, Dalila; PRAPAYOTIN‐RIVEROS, KITTIPATH; Quilichini, Angélique; Reynaud, Bernard; Riginos, Cynthia; Risterucci, Ange Marie; Rose, H. A.; Sampaio, Iracilda; Silbermayr, Katja; SILVA, M. B.; Tero, N.; Thum, Ryan A.; Vinson, C. C.; Vorsino, Adam E.; Vossbrinck, Charles R.; Walzer, Chris; White, Jason C.; Wieczorek, Ania M.; Wright, Mark G.

doi:10.1111/j.1755-0998.2010.02916.x

Cited by 43 publications

(21 citation statements)

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“…The remaining thirteen were newly identified SSRs from D . alata [39]. Microsatellite alleles were scored using the software GeneMapper 4.0 (Applied Byosystems, USA).…”

Section: Methodsmentioning

confidence: 99%

Understanding the genetic diversity and population structure of yam (Dioscorea alata L.) using microsatellite markers

Arnau

Bhattacharjee

et al. 2017

PLoS ONE

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Yams (Dioscorea sp.) are staple food crops for millions of people in tropical and subtropical regions. Dioscorea alata, also known as greater yam, is one of the major cultivated species and most widely distributed throughout the tropics. Despite its economic and cultural importance, very little is known about its origin, diversity and genetics. As a consequence, breeding efforts for resistance to its main disease, anthracnose, have been fairly limited. The objective of this study was to contribute to the understanding of D. alata genetic diversity by genotyping 384 accessions from different geographical regions (South Pacific, Asia, Africa and the Caribbean), using 24 microsatellite markers. Diversity structuration was assessed via Principal Coordinate Analysis, UPGMA analysis and the Bayesian approach implemented in STRUCTURE. Our results revealed the existence of a wide genetic diversity and a significant structuring associated with geographic origin, ploidy levels and morpho-agronomic characteristics. Seventeen major groups of genetically close cultivars have been identified, including eleven groups of diploid cultivars, four groups of triploids and two groups of tetraploids. STRUCTURE revealed the existence of six populations in the diploid genetic pool and a few admixed cultivars. These results will be very useful for rationalizing D. alata genetic resources in breeding programs across different regions and for improving germplasm conservation methods.

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“…The remaining thirteen were newly identified SSRs from D . alata [39]. Microsatellite alleles were scored using the software GeneMapper 4.0 (Applied Byosystems, USA).…”

Section: Methodsmentioning

confidence: 99%

Understanding the genetic diversity and population structure of yam (Dioscorea alata L.) using microsatellite markers

Arnau

Bhattacharjee

et al. 2017

PLoS ONE

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“…1) were selected among the 14 previously developed by our team for M. sacchari [35]. PCR reactions were performed with labelled primers and multiplexed into two mixes (Type-it, standard procedure, Qiagen), and the following thermocycling protocol was used: denaturation at 95°C for 15 min, 25 denaturation cycles for 30 s at 94°C, a 1-min 30 s annealing step at 54°C, and a 30-s elongation step at 72°C.…”

Section: Methodsmentioning

confidence: 99%

Low Genetic Diversity in Melanaphis sacchari Aphid Populations at the Worldwide Scale

et al. 2014

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Numerous studies have examined the genetic diversity and genetic structure of invading species, with contrasting results concerning the relative roles of genetic diversity and phenotypic plasticity in the success of introduced populations. Increasing evidence shows that asexual lineages of aphids are able to occupy a wide geographical and ecological range of habitats despite low genetic diversity. The anholocyclic aphid Melanaphis sacchari is a pest of sugarcane and sorghum which originated in the old world, was introduced into the Americas, and is now distributed worldwide. Our purpose was to assess the genetic diversity and structuring of populations of this species according to host and locality. We used 10 microsatellite markers to genotype 1333 individuals (57 samples, 42 localities, 15 countries) collected mainly on sugarcane or sorghum. Five multilocus lineages (MLL) were defined, grouping multilocus genotypes (MLG) differing by only a few mutations or scoring errors. Analysis of a 658 bp sequence of mitochondrial COI gene on 96 individuals revealed five haplotypes, with a mean divergence of only 0.19 %. The distribution of MLL appeared to be strongly influenced by geography but not by host plant. Each of the five MLL grouped individuals from (A) Africa, (B) Australia, (C) South America, the Caribbean and the Indian Ocean including East Africa, (D) USA, and (E) China. The MLL A and C, with a wide geographic distribution, matched the definition of superclone. Among aphids, M. sacchari has one of the lowest known rates of genetic diversity for such a wide geographical distribution.

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“…Seventy-five SSRs developed from five different yam species – D. rotundata , D. abyssinica , D. prahensilis , D. japonica and D. alata (Misuki et al , 2005; Tostain et al , 2006; Andris et al , 2010) – were used to determine the genotypes of the genitors. Only the six SSR markers that revealed polymorphism between the diploid parents ‘5F’ and ‘Kabusa’, without any common allele (Da2F10, Da1D08, mDaCIR8, mDaCIR60, mDaCIR61 and mDrCIR128), were selected.…”

Section: Methodsmentioning

confidence: 99%

Microsatellite and flow cytometry analysis to help understand the origin of Dioscorea alata polyploids

Némorin

David²,

Maledon

et al. 2013

Annals of Botany

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Background and Aims Dioscorea alatais a polyploid species with a ploidy level ranging from diploid (2n = 2x = 40) to tetraploid (2n = 4x = 80). Ploidy increase is correlated with better agronomic performance. The lack of knowledge about the origin of D. alata spontaneous polyploids (triploids and tetraploids) limits the efficiency of polyploid breeding. The objective of the present study was to use flow cytometry and microsatellite markers to understand the origin of D. alata polyploids.MethodsDifferent progeny generated by intracytotype crosses (2x × 2x) and intercytotype crosses (2x × 4x and 3x × 2x) were analysed in order to understand endosperm incompatibility phenomena and gamete origins via the heterozygosity rate transmitted to progeny.ResultsThis work shows that in a 2x × 2x cross, triploids with viable seeds are obtained only via a phenomenon of diploid female non-gametic reduction. The study of the transmission of heterozygosity made it possible to exclude polyspermy and polyembryony as the mechanisms at the origin of triploids. The fact that no seedlings were obtained by a 3x × 2x cross made it possible to confirm the sterility of triploid females. Flow cytometry analyses carried out on the endosperm of seeds resulting from 2x × 4x crosses revealed endosperm incompatibility phenomena.ConclusionsThe major conclusion is that the polyploids of D. alata would have appeared through the formation of unreduced gametes. The triploid pool would have been built and diversified through the formation of 2n gametes in diploid females as the result of the non-viability of seeds resulting from the formation of 2n sperm and of the non-viability of intercytotype crosses. The tetraploids would have appeared through bilateral sexual polyploidization via the union of two unreduced gametes due to the sterility of triploids.

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Permanent Genetic Resources added to Molecular Ecology Resources Database 1 June 2010 – 31 July 2010

Cited by 43 publications

References 0 publications

Understanding the genetic diversity and population structure of yam (Dioscorea alata L.) using microsatellite markers

Understanding the genetic diversity and population structure of yam (Dioscorea alata L.) using microsatellite markers

Low Genetic Diversity in Melanaphis sacchari Aphid Populations at the Worldwide Scale

Microsatellite and flow cytometry analysis to help understand the origin of Dioscorea alata polyploids

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