Permanent effects of estradiol on cellular metabolism of the developing mouse vagina

Kohrman, Arthur F.; Greenberg, Robert E.

doi:10.1016/0012-1606(68)90030-4

Cited by 24 publications

(13 citation statements)

References 19 publications

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“…The vaginal epithelium of female mice given injec tions of estrogenic or androgenic steroids during a criti cal neonatal period showed estrogen-independent per sistent proliferation and cornification [Takasugi, 1963;Kohrman and Greenberg, 1968;Cunha et al, 1977; for a review, see Takasugi, 1976). Histological and histochemical studies have revealed no difference in the final structure of the vagi nal epithelium between neonatally estrogen-and androgen-treated mice.…”

Section: Introductionmentioning

confidence: 99%

Electron-microscopic study on the development of permanently proliferated and cornified vaginal epithelium in mice treated neonatally with androgen

Iguchi¹,

Ohta²

1980

Cells Tissues Organs

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In the vaginal epithelium of mice given 5 daily injections of 100 µg 5α-dihydrotestosterone from the day of birth, highly electron-dense cells with numerous cytoplasmic processes appeared in the basal layer within 35 days after the last injection. These cells were similar to the B cells reported in earlier works in neonatally estrogen-treated mice undergoing ovary-independent, permanent proliferation and cornification. The present electron-microscopic study suggests that the permanently proliferating, highly electron-dense cells are derived from electronlucent cells (clear cells) appearing in the basal layer of the vaginal epithelium 15–20 days after neonatal androgen treatment.

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Section: Introductionmentioning

confidence: 99%

Electron-microscopic study on the development of permanently proliferated and cornified vaginal epithelium in mice treated neonatally with androgen

Iguchi¹,

Ohta²

1980

Cells Tissues Organs

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“…The Bcell membrane bore fewer desmosomes than in !he basal and intermediary cells of the vaginal epithelium of ovariectomized 'normal' adult mice after 5 daily injections of 100 pg estradioL17P. Hyperplastic epithelial downgrowths in old ovariectomized mice given neonatal estrogen injections contained another type of cells with reduced density which formed much fewer processes and only a few desmosomes (C-cells).Five to ten daily injections of estrogen starting on the day of birth cause proliferation and cornification of the mouse vaginal epithelium, which persist without further administration of estrogen (TAKASUGI, 1963; KIMURA et al, 1967;MORI, 1967;KOHRMAN and GREENBERG, 1968; for review, see TAKASUGI et ul., 1970). These changes in the vaginal epithelium are irreversible, frequently resulting in cancerous or precancerous lesions in neonatally estrogenized mice of more than 6 months of age (DUNN and GREEN, 1963; TAKASUGI and BERN, 1964; KIMURA and NANDI, 1967; MORI, 1969).…”

mentioning

confidence: 99%

“…Five to ten daily injections of estrogen starting on the day of birth cause proliferation and cornification of the mouse vaginal epithelium, which persist without further administration of estrogen (TAKASUGI, 1963; KIMURA et al, 1967;MORI, 1967;KOHRMAN and GREENBERG, 1968; for review, see TAKASUGI et ul., 1970). These changes in the vaginal epithelium are irreversible, frequently resulting in cancerous or precancerous lesions in neonatally estrogenized mice of more than 6 months of age (DUNN and GREEN, 1963; TAKASUGI and BERN, 1964; KIMURA and NANDI, 1967; MORI, 1969).…”

mentioning

confidence: 99%

Development of Vaginal Epithelium Showing Irreversible Proliferation and Cornification in Neonatally Estrogenized Mice: An Electron Microscope Study *

Takasugi¹,

Kamishima²

1973

Dev Growth Differ

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Irreversible proliferation and cornification of the mouse vaginal epithelium were induced by 10 daily injections of 20 pg estradioLl7p starting on the day of birth. Development of the irreversible vaginal epithelium during the period of estrogen injections in early postnatal life was observed under light and electron microscopes, Small electron-dense cells (A-cells) in clusters were present in the columnar vaginal epithelium of newborn mice. A-cells were proliferated by 2 daily estrogen injections. At the sites of A-cell clumps, large electron-dense cells (B-cells) characterized by long winding cytoplasmic processes appeared in mice given 3 daily injections, forming nodules which then fused together to form a layer under the columnar epithelium after 4 daily injections. In mice given 7 daily injections, the primary epithelium was shed by the superficial cornification of the newly formed layer. The Bcell membrane bore fewer desmosomes than in !he basal and intermediary cells of the vaginal epithelium of ovariectomized 'normal' adult mice after 5 daily injections of 100 pg estradioL17P. Hyperplastic epithelial downgrowths in old ovariectomized mice given neonatal estrogen injections contained another type of cells with reduced density which formed much fewer processes and only a few desmosomes (C-cells).Five to ten daily injections of estrogen starting on the day of birth cause proliferation and cornification of the mouse vaginal epithelium, which persist without further administration of estrogen (TAKASUGI, 1963; KIMURA et al., 1967;MORI, 1967;KOHRMAN and GREENBERG, 1968; for review, see TAKASUGI et ul., 1970). These changes in the vaginal epithelium are irreversible, frequently resulting in cancerous or precancerous lesions in neonatally estrogenized mice of more than 6 months of age (DUNN and GREEN, 1963; TAKASUGI and BERN, 1964; KIMURA and NANDI, 1967; MORI, 1969).Recently, TAKASUCI (1 97 1) has demonstrated that clumps of small-sized cells

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“…Similar changes have been reported in female rodents treated neonatally (i.e. at the time of sexual differentiation) with oestrogen or testosterone (Kohrman & Greenberg, 1968;Takasugi & Kamishima, 1973). Such animals also have decreased female sexual behaviour and increased male behaviour (Phoenix, Goy, Gerall & Young, 1959;Gorski, 1973;Whalen & Etgen, 1978) and increased aggressive behaviour (Bronson & Desjardins, 1970) when stimulated with hormonal steroids during adult life.…”

Section: Discussionmentioning

confidence: 54%

Sexual behaviour of ewes with clover disease treated repeatedly with oestradiol benzoate or testosterone propionate after ovariectomy

Adams¹

1983

Reproduction

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Ovariectomized ewes, 14 with permanent clover disease infertility (affected ewes) and 14 controls, were injected daily with 40 micrograms oestradiol benzoate for 12 days, and run with 2 rams fitted with marking crayons. The control ewes were mated sooner (P less than 0.05) but both groups became refractory at a similar rate. In a second experiment, 20 similar affected ewes and 19 controls were injected daily with 5 mg testosterone propionate for 31 days and observed daily for 50 min with rams. Affected ewes again were slower to show female behaviour (P less than 0.05) but faster (P less than 0.05) to show aggression against the rams and other ewes. Over the 31 days, the incidence of female sexual behaviour declined at a similar rate in affected and control ewes. When examined in individual pen tests with oestrous ewes on Day 28, affected ewes showed more male-like courting behaviour than did controls (P less than 0.05). The changes in behaviour are too slight to account for the infertility but they do support the hypothesis that phyto-oestrogens can act on the ewe by some of the pathways of sexual differentiation, even after puberty.

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Permanent effects of estradiol on cellular metabolism of the developing mouse vagina

Cited by 24 publications

References 19 publications

Electron-microscopic study on the development of permanently proliferated and cornified vaginal epithelium in mice treated neonatally with androgen

Electron-microscopic study on the development of permanently proliferated and cornified vaginal epithelium in mice treated neonatally with androgen

Development of Vaginal Epithelium Showing Irreversible Proliferation and Cornification in Neonatally Estrogenized Mice: An Electron Microscope Study *

Sexual behaviour of ewes with clover disease treated repeatedly with oestradiol benzoate or testosterone propionate after ovariectomy

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