Permanent Coverage of Large Burn Wounds with Autologous Cultured Human Epithelium

Gallico, G. Gregory; O’Connor, Nick; Compton, Carolyn C.; Kehinde, Olaniyi; Green, Howard

doi:10.1056/nejm198408163110706

Cited by 1,155 publications

(236 citation statements)

References 13 publications

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“…Mostly natural polysaccharides have been investigated as biomaterials, such as chitosan [5,7,31], cellulose [32], collagen [33,34], and hyaluronic acid [35][36][37]. Chitin/poly(lactic acid-co-glycolic acid) (PGLA) blends [38] and starch-based blends [39,40] have been reported for protein drug delivery and tissue engineering scaffolds, respectively.…”

Section: Introductionmentioning

confidence: 99%

Biodegradable Polysaccharide Gels for Skin Scaffolds

Juris¹,

Mueller²,

Smith³

et al. 2011

JBNB

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Section: Introductionmentioning

confidence: 99%

Biodegradable Polysaccharide Gels for Skin Scaffolds

Juris¹,

Mueller²,

Smith³

et al. 2011

JBNB

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“…Success has been achieved with freshly isolated hematopoietic stem cells for lethally irradiated recipients and hepatocytes in models of liver degeneration (1)(2)(3)(4). In only a few cases have long-term cultivable cells been demonstrated to effect tissue repair, including keratinocytes for skin grafts (5) and mesangioblasts or musclederived stem cells for muscle repair of dystrophic mice (6,7).…”

mentioning

confidence: 99%

Bipotential mouse embryonic liver stem cell lines contribute to liver regeneration and differentiate as bile ducts and hepatocytes

Strick‐Marchand

Morosan

Charneau

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

101

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“…Keratinocytes can generate cohesive sheets of stratified epithelium that maintains virtually the same differentiation features and gene expression pattern of its in vivo counterpart so that it can be routinely transplanted in patients suffering from large skin or mucosal defects (31)(32)(33). When primary keratinocytes are seeded onto dead de-epidermized dermis in organotypic cultures (29), mature HDs are formed in vitro (30).…”

mentioning

confidence: 99%

Gene Correction of Integrin β4-dependent Pyloric Atresia-Junctional Epidermolysis Bullosa Keratinocytes Establishes a Role for β4 Tyrosines 1422 and 1440 in Hemidesmosome Assembly

Dellambra¹,

Prislei²,

Salvati³

et al. 2001

Journal of Biological Chemistry

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The cytoplasmic domain of ␤ 4 integrin contains two pairs of fibronectin-like repeats separated by a connecting segment. The connecting segment harbors a putative tyrosine activation motif in which tyrosines 1422 and 1440 are phosphorylated in response to ␣ 6 ␤ 4 binding to laminin-5. Primary ␤ 4 -null keratinocytes, obtained from a newborn suffering from lethal junctional epidermolysis bullosa, were stably transduced with retroviruses carrying a full-length ␤ 4 cDNA or a ␤ 4 cDNA with phenylalanine substitutions at Tyr-1422 and Tyr-1440. Hemidesmosome assembly was evaluated on organotypic skin cultures. ␤ 4 -corrected keratinocytes were indistinguishable from normal cells in terms of ␣ 6 ␤ 4 expression, the localization of hemidesmosome components, and hemidesmosome structure and density, suggesting full genetic and functional correction of ␤ 4 -null keratinocytes. In cultures generated from ␤ 4 Y1422F/Y1440Fkeratinocytes, ␤ 4 mutants as well as ␣ 6 integrin, HD1/ plectin, and BP180 were not concentrated at the dermalepidermal junction. Furthermore, the number of hemidesmosomes was strikingly reduced as compared with ␤ 4 -corrected keratinocytes. The rare hemidesmosomes detected in ␤ 4 Y1422F/Y1440F cells were devoid of subbasal dense plates and of inner cytoplasmic plaques with keratin filament insertion. Collectively, our data demonstrate that the ␤ 4 tyrosine activation motif is not required for the localization of ␣ 6 ␤ 4 at the keratinocyte plasma membrane but is essential for optimal assembly of bona fide hemidesmosomes.Human epidermis consists of a stratified squamous epithelium composed of keratinocytes organized in distinct cellular layers. Keratinocytes forming the basal layer firmly adhere to the basement membrane by means of hemidesmosomes (HDs), 1 multiprotein complexes linking the epithelial intermediate filament network to the dermal anchoring fibrils (see Refs. 1 and 2 for review). HDs are formed by the clustering of several cytoplasmic and trans-membrane proteins (2). The cytoplasmic HD plaque components, which include HD1/plectin (3) and the bullous pemphigoid antigen 1 (BP230) (4), act as linkers for elements of the cytoskeleton at the cytoplasmic surface of plasma membrane. The trans-membrane constituents of HDs, which include the ␣ 6 ␤ 4 integrin (5, 6) and the bullous pemphigoid antigen 2 (BP180) (7), serve as cell receptors connecting the cell interior to extracellular matrix proteins.In particular, the ␣ 6 ␤ 4 integrin is a receptor for the basement membrane component laminin-5, a heterotrimeric protein composed of three distinct polypeptides, ␣3, ␤3, and ␥2, which are encoded by three different genes known as LAMA3, LAMB3, and LAMC2, respectively (see Ref. 8 for review). Laminin-5 binds to the basal keratinocyte cell surface through the ␣ 6 ␤ 4 integrin and tightens the dermal-epidermal junction by binding also to the N-terminal NC-1 domain of type VII collagen (9). The crucial importance of the interaction between laminin-5 and its ␣ 6 ␤ 4 receptor in maintaining the integrity of the i...

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Permanent Coverage of Large Burn Wounds with Autologous Cultured Human Epithelium

Cited by 1,155 publications

References 13 publications

Biodegradable Polysaccharide Gels for Skin Scaffolds

Biodegradable Polysaccharide Gels for Skin Scaffolds

Bipotential mouse embryonic liver stem cell lines contribute to liver regeneration and differentiate as bile ducts and hepatocytes

Gene Correction of Integrin β4-dependent Pyloric Atresia-Junctional Epidermolysis Bullosa Keratinocytes Establishes a Role for β4 Tyrosines 1422 and 1440 in Hemidesmosome Assembly

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