Permanent cannulation of aorta and vena cava in rats and ground squirrels

Popović, Vladimir; Popovic, Pava

doi:10.1152/jappl.1960.15.4.727

Cited by 340 publications

(103 citation statements)

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“…Blood pressure (1 mmHg = 1.33 mbar) was recorded from aortic catheters implanted, under halothane anaesthesia, by the techniques of Popovic & Popovic (1960) or Weeks & Jones (1960). Experiments were done not less than 24 h after surgery.…”

Section: Methodsmentioning

confidence: 99%

HYPOTENSIVE AND VASODILATOR ACTIONS OF SK&F 24260, A NEW DIHYDROPYRIDINE DERIVATIVE

Fielden¹,

Owen²,

Taylor³

1974

British J Pharmacology

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1 The blood pressure of conscious normotensive, Goldblatt-hypertensive and genetichypertensive rats and normotensive dogs was lowered by SK&F 24260 (1,4-dihydro-2,6-dimethyl-4-(2-trifluormethylphenyl)-3,5-pyridinedicarboxylic acid diethyl ester). 2 Tachycardia always accompanied the hypotension. In rats propranolol abolished the tachycardia but in dogs there was only a small reduction in the heart rate response. 3 In conscious dogs there was an increase in left ventricular output and a marked fall in total peripheral resistance. 4 SK&F 24260 dilated resistance vessels in rat hindquarters. Dilatation was caused by doses of SK&F 24260 some 50 times smaller than those of hydrallazine. 5 In preparations of cat skeletal muscle and intestinal vascular beds SK&F 24260 selectively dilated resistance vessels in preference to capacitance vessels and resembled hydrallazine rather than papaverine which has no such selectivity. 6 Pre-capillary sphincter vessels were also dilated by SK&F 24260. Changes in fluid equilibrium caused by SK&F 24260 were consistent with selective dilatation of resistance vessels.

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Section: Methodsmentioning

confidence: 99%

HYPOTENSIVE AND VASODILATOR ACTIONS OF SK&F 24260, A NEW DIHYDROPYRIDINE DERIVATIVE

Fielden¹,

Owen²,

Taylor³

1974

British J Pharmacology

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“…The catheter was aseptically implanted in animals anaesthetized with halothane (Halocarbon Labs, Hackensack, NJ). The catheter was filled with heparinized saline (500 U/ml) and implanted as previously described (Papovic & Papovic, 1960). Surgery, including Field, White & Lang anaesthesia, was completed in 12-15 min.…”

Section: Phase Iii: Cardiovascular and Respiratory Effects Arterial Cmentioning

confidence: 99%

Anaesthetic effects of chloral hydrate, pentobarbitone and urethane in adult male rats

1993

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SummaryChloral hydrate, pentobarbitone and urethane were evaluated and compared for onset, duration and depth of anaesthesia, cardiovascular and respiratory effects, nociception and mortality in adult male rats. Chloral hydrate (300 and 400 mg/kg) severely depressed the cardiovascular and respiratory systems. Duration of anaesthesia was linearly related to dose, and anaesthetic depth and analgesia were excellent. Pentobarbital (40 mg/kg) produced a short period of light surgical anaesthesia. Moderate to severe respiratory and cardiovascular depression occurred. Duration of anaesthesia was not related to dose. Urethane (1' 2 and 1· 5 g/kg) caused moderate cardiovascular depression. In addition, mortality was high at the 1·5 g/kg dose. Duration of anaesthesia was greater than 24 h for most animals. Anaesthesia depth and analgesia were excellent. Keywords: Anaesthesia; Nociception; Chloral hydrate; Pentobarbitone; UrethaneRecent studies have shown that the depth of anaesthesia, analgesia, and the physiological properties of injectable agents used in rats are often not characterized adequately (Wixson et aI.,

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“…Arterial and right heart cannulas were placed through the carotid artery and the external jugular vein respectively, according to Popovic & Popovic (1960). Briefly, implantation of the cannulas was carried out through an incision in the ventrolateral position of the neck.…”

Section: Insertion Of Cannulas Into Aorta and Right Heartmentioning

confidence: 99%

A method for sampling representative muscular venous blood during exercise in rats

et al. 1993

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SummaryA technique for chronic cannulation of the muscular branch of the femoral vein in the rat is described. The method was validated by the application of vascular corrosion casts and comparative analysis of lactate concentration with mixed venous blood and arterial samples taken through the cannulas during lower hindlimb muscle contraction in anaesthetized rats. Keywords: Skeletal muscle; Corrosion cast; Lactate, Arterio-venous difference; Blood samplingThe comparison of arterial and venous blood samples from thigh muscles could be useful for the study of biochemical and metabolic muscle responses to exercise in rats during experiments involving the lower hindlimb, since this allows the determination of several parameters of arterio-venous differences that undergo alterations during exercise, such as oxygen consumption, gas exchange rate, pH, lactate, ammonium and others.A technique is described for obtaining representative sequential blood samples from thigh muscle areas. A cannula was inserted through the muscular branch tributary of the femoral vein. Implantation of the cannula does not block circulation and allows withdrawal of venous blood from skeletal muscles of the lower hindlimb. Moreover, simultaneous cannulation of the aorta and right heart (which has been Correspondence to: T Pages. 1992; accepted 19 October 1992 considered as representative of mixed venous blood) allows the comparison of blood composition at 3 locations in order to study the arterio-venous difference during training exercise before and after mixing of lower hindlimb muscular venous blood with cephalic and splanchnic circulatory return. Received 3 JulyTo verify the origin of blood withdrawn from the femoral vein by means of this technique, a vascular corrosion cast method was used to provide the complete arterio-venous tributary tree from the cannulated vessel. A report of changes in regional lactate blood concentration during lower hindlimb exerciseinduced by electric stimulation is also given. Material and methods AnimalsWistar adult male rats weighing 250-300 g were used. Animals were housed in propylene cages (Technoplast, Italy) at 21 ± O·5°C, 700/0humidity and 12 h L-D cycle, and fed laboratory chow (Panlab A4, Spain) and tap water ad libitum.

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Permanent cannulation of aorta and vena cava in rats and ground squirrels

Cited by 340 publications

References 0 publications

HYPOTENSIVE AND VASODILATOR ACTIONS OF SK&F 24260, A NEW DIHYDROPYRIDINE DERIVATIVE

HYPOTENSIVE AND VASODILATOR ACTIONS OF SK&F 24260, A NEW DIHYDROPYRIDINE DERIVATIVE

Anaesthetic effects of chloral hydrate, pentobarbitone and urethane in adult male rats

A method for sampling representative muscular venous blood during exercise in rats

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