Perkinsus sp. infecting oyster Crassostrea rhizophorae (Guilding, 1828) on the coast of Bahia, Brazil

Brandão, Rosana Pinho; Boehs, Guisla; Sabry, Rachel Costa; Ceuta, L O; Luz, Mariane dos Santos Aguiar; Queiroga, Fernando Ramos; Silva, Patrícia Mirella da

doi:10.1016/j.jip.2012.11.003

Cited by 27 publications

(23 citation statements)

References 7 publications

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“…The study complements reports published by Sabry et al (2009), Silva et al (2013) and Brandão et al (2013) on the occurrence of this pathogen in the states of Ceará, Paraíba and Bahia, respectively. In addition, the study makes a significant contribution to current knowledge about the distribution of Perkinsus along the Brazilian coast and provides subsidies for the development of sanitary measures to prevent contamination of protozoan-free areas.…”

Section: Introductionsupporting

confidence: 85%

“…Histological sections of oyster tissues contained cells of Perkinsus sp. with a vacuole occupying most of the internal volume of the cytoplasm, an eccentric nucleus and prominent nucleoli, as described by Sabry et al (2009Sabry et al ( , 2013 and Brandão et al (2013). These authors detected changes in oyster tissues and organs infected by Perkinsus sp.…”

Section: Discussionmentioning

confidence: 62%

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Perkinsus sp. infecting the oyster Crassostrea rhizophorae from estuaries of the septentrional Northeast, Brazil

Dantas-Neto

Sabry²,

Ferreira

et al. 2015

Braz. J. Biol.

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The mangrove oyster Crassostrea rhizophorae is an estuarine resource exploited by riverside communities in Northeast Brazil. Despite its socioeconomic importance, studies on the health status of this bivalve are scanty in this region. The purpose of the present study was to investigate the presence of the protozoan Perkinsus sp. in C. rhizophorae collected in August and September 2011 in three estuaries of the septentrional Northeast, Brazil: Jaguaribe (Ceará), Camurupim (Piauí) and Carnaubeiras (Maranhão) (n= 150 specimens/site). The samples were submitted to Ray's fluid thioglycollate medium (RFTM), PCR and histology assays. The RFTM assay revealed spherical, blue or bluish-black hypnospores of the genus Perkinsus in 50 specimens (Jaguaribe= 17.3%, Camurupim= 5.3%, Carnaubeiras= 10.6%). The intensity of the infection ranged from very light (1-10 cells per slide) to severe (more than 40 cells in each of 10 fields of the slide) for Jaguaribe; very light for Camurupim and very light to moderate (at least 40 cells observed in each of 10 fields of the slide) for Carnaubeiras. When submitted to confirmatory PCR analysis, 6 cases were confirmed (Jaguaribe=3, Camurupim=1, Carnaubeiras=2). The histology confirmed 21 cases of infection in specimens from the three estuaries. Although local collectors have reported no mortality in oyster populations that might be attributed to infection by Perkinsus, health surveillance of oyster populations in the septentrional region of Northeast Brazil is advisable.Keywords: Perkinsus, Crassostrea rhizophorae, RFTM, PCR. Perkinsus sp. infectando a ostra Crassostrea rhizophorae de estuários do Nordeste setentrional, Brasil ResumoA ostra-do-mangue Crassostrea rhizophorae é um recurso estuarino explorado por comunidades ribeirinhas do Nordeste do Brasil. Apesar de sua importância socioeconômica, estudos sobre o estado de saúde deste bivalve são escassos na região. O objetivo deste estudo foi investigar a presença do protozoário Perkinsus sp. em C. rhizophorae coletada em agosto e setembro de 2011, em três estuários da região setentrional do Nordeste brasileiro: Jaguaribe (Ceará), Camurupim (Piauí) e Carnaubeiras (Maranhão) (n = 150 espécimes/local). As amostras foram submetidas ao meio líquido de tioglicolato de Ray (RFTM), PCR e ensaios histológicos. A análise em RFTM revelou hipnósporos esféricos azuis ou preto-azulados do gênero Perkinsus em 50 espécimes (Jaguaribe= 17,3%, Camurupim= 5,3%, Carnaubeiras= 10,6%). A intensidade de infecção variou de muito leve (1-10 células por lâmina) a severa (mais de 40 células em cada um dos 10 campos da lâmina) para o Rio Jaguaribe; muito leve para o Rio Camurupim e muito leve a moderada (pelo menos 40 células observadas, em cada um dos 10 campos da lâmina) para o Rio Carnaubeiras. Quando submetidos à análise confirmatória por PCR, foram confirmados 6 casos (Jaguaribe= 3, Camurupim= 1, Carnaubeiras= 2). A histologia confirmou 21 casos de infecção em espécimes dos três estuários. Embora os coletores locais não tenham relatado nenhuma mortalida...

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Section: Introductionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 62%

Perkinsus sp. infecting the oyster Crassostrea rhizophorae from estuaries of the septentrional Northeast, Brazil

Dantas-Neto

Sabry²,

Ferreira

et al. 2015

Braz. J. Biol.

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“…in oysters seems to be associated with salinity, i.e. the prevalence and parasite burden decrease at low salinities, which usually occurs in the rainy season (BRANDÃO et al, 2013;SILVA et al, 2014;QUEIROGA et al, 2015). Accordingly, in the shrimp pond, high salinity (37 to 40 psu) accumulated during November 2014 to February 2015, could have contributed to the highest prevalence of Perkinsus spp.…”

Section: Discussionmentioning

confidence: 99%

Epizootiology of Perkinsus sp. inCrassostrea gasar oysters in polyculture with shrimps in northeastern Brazil

Silva

Costa

Araújo

et al. 2016

Rev. Bras. Parasitol. Vet.

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Bivalve culture is of considerable economic and social interest in northeastern (NE) Brazil. The polyculture is an alternative approach to traditional monoculture for reducing the environmental impact of shrimp farming and improving oyster culture. Perkinsus marinus and Perkinsus olseni were found infecting oysters in NE Brazil and can threaten oyster production. This study evaluated Perkinsus spp. occurrence in Crassostrea gasar during all production stages. Oyster spats were produced in a hatchery and grown in shrimp ponds in Rio Grande do Norte state. Perkinsus spp. were surveyed by Ray's fluid thioglycollate medium and confirmed by polymerase chain reaction. Prevalence and intensity of infection were determined in oysters until they reached 7 cm. Results showed that the broodstock was already infected by Perkinsus (60%), but the derived spats were Perkinsus-free. Oyster spats acquired Perkinsus infection when transferred to ponds. The prevalence gradually increased in the seven months following placement in ponds (73%), and then decreased to 17% by the tenth month. The infections were initially mild, but intensity increased at the final growth stage. In conclusion, it is possible to produce Perkinsus-free C. gasar oyster spats from infected broodstock, and their culture in shrimp ponds is feasible.Keywords: Crassostrea gasar, hatchery, oyster, polyculture, Perkinsus sp., sanitary control. ResumoO cultivo de bivalves é de grande interesse econômico e social no Nordeste (NE) do Brasil. O policultivo é uma alternativa ao monocultivo tradicional de camarões para reduzir o impacto ambiental e melhorar a produção de ostras. Perkinsus marinus e Perkinsus olseni foram identificados infectando ostras no Nordeste do Brasil e representam uma ameaça a produção de ostras. Este estudo avaliou a ocorrência de Perkinsus spp. em Crassostrea gasar durante todas as fases de produção. Sementes de ostras foram produzidas em laboratório e cultivadas em viveiros de camarão no Rio Grande do Norte. Perkinsus spp. foram diagnosticados com o uso do meio de tioglicolato fluido de Ray e confirmado por reação em cadeia da polimerase. A prevalência e intensidade de infecção foram determinadas em ostras até atingirem 7 cm. Os resultados mostraram que os reprodutores encontravam-se infectados por Perkinsus (60%), mas as sementes produzidas estavam livres de Perkinsus. As sementes adquiriram a infecção por Perkinsus quando transferidas para os viveiros. A prevalência aumentou gradualmente nos sete meses após a colocação nos viveiros (73%) e, em seguida, diminuiu para 17% até o décimo mês. As infecções foram inicialmente leves, mas aumentaram até a fase final do crescimento. Em conclusão, é possível produzir sementes de ostras C. gasar livres de Perkinsus a partir de reprodutores infectados e seu cultivo em viveiros de camarão é viável.Palavras-chave: Crassostrea gasar, unidade de reprodução, ostras, policultivo, Perkinsus sp., controle sanitário.

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“…According to Sande et al (2010) and Brandão et al (2013), both the Cachoeira River estuary and Camamu Bay are considered suitable areas for extracting mollusks, which are used as a source of food for the coastal population, as well as for sale during the high season, as summer. Bivalve mollusks reflect the conditions in which they live and are considered bioindicators of environmental contamination (Miller et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

Detection of Toxoplasma gondii DNA in Brazilian oysters (Crassostrea rhizophorae)

et al. 2015

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ABSTRACT. The aim of this study was to detect evidence of Toxoplasma gondii using polymerase chain reaction (PCR)-based techniques in oysters (Crassostrea rhizophorae) obtained from the southern coastal region of Bahia, Brazil. A total of 624 oysters were collected, and the gills and digestive glands were dissected. Each tissue sample was separated into pools containing tissues (of the same type) from three animals, leading to a total of 416 experimental samples for analysis (208 samples each from the gills and digestive glands). Molecular analysis using PCR-based detection of the T. gondii AF 146527 repetitive fragment yielded negative results for all samples. However, when nested-PCR was used for detection of the T. gondii SAG-1 gene, 17 samples were positive, with the gills being the tissue with maximal detection of the parasite. These positive results were confirmed by sample sequencing. It is therefore suggested that C. rhizophorae oysters are capable of filtering and retaining T. gondii oocysts in their tissue. This represents a risk to public health because they are traditionally ingested in natura.

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Perkinsus sp. infecting oyster Crassostrea rhizophorae (Guilding, 1828) on the coast of Bahia, Brazil

Cited by 27 publications

References 7 publications

Perkinsus sp. infecting the oyster Crassostrea rhizophorae from estuaries of the septentrional Northeast, Brazil

Perkinsus sp. infecting the oyster Crassostrea rhizophorae from estuaries of the septentrional Northeast, Brazil

Epizootiology of Perkinsus sp. inCrassostrea gasar oysters in polyculture with shrimps in northeastern Brazil

Detection of Toxoplasma gondii DNA in Brazilian oysters (Crassostrea rhizophorae)

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