Perivascular peptides relax cerebral arteries concomitant with stimulation of cyclic adenosine monophosphate accumulation or release of an endothelium-derived relaxing factor in the cat

Edvinsson, Lars; Fredholm, Bertil B.; Hamel, Edith; Jansen, I.; Verrecchia, C.

doi:10.1016/0304-3940(85)90166-1

Cited by 362 publications

(155 citation statements)

References 7 publications

Supporting

Mentioning

143

Contrasting

Unclassified

Order By: Relevance

“…This indicates that the response to CGRP is mediated mainly by a direct effect on the vascular smooth muscle. Reports on CGRP show both an endothelium-dependent pathway (Brain et al 1985, Grace et al 1987 and an endothelium-independent pathway (Edvinsson et al 1985, Brizzolara and Burnstock 1991, Prieto et al 1991, Samuelson and Jernbeck 1991. This may imply variations between different vascular beds and species.…”

Section: Discussionmentioning

confidence: 99%

Vasorelaxation in isolated bone arteries: Vasoactive intestinal peptide, substance P, calcitonin gene-related peptide bradykinin studied in pigs

Lundgaard¹,

Aalkjær²,

Bjurholm

et al. 1997

Acta Orthopaedica Scandinavica

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Vasorelaxation in isolated bone arteries: Vasoactive intestinal peptide, substance P, calcitonin gene-related peptide bradykinin studied in pigs

Lundgaard¹,

Aalkjær²,

Bjurholm

et al. 1997

Acta Orthopaedica Scandinavica

View full text Add to dashboard Cite

“…Calcitonin gene-related peptide (CGRP) is a very potent vasodilator neuropeptide in many isolated blood vessels from both man and rat (Brain et al, 1985;Edvinsson et al, 1985;Mulderry et al, 1985). We have previously shown that plasma levels of CGRP are significantly elevated in rats and pigs following injection of endotoxin, and in a rat model of haemorrhagic shock (Wang et al, 1991;Arden et al, 1994); in addition, plasma CGRP levels were elevated in septic shock patients (Joyce et al, 1990).…”

Section: Introductionmentioning

confidence: 99%

Rapid nitric oxide‐ and prostaglandin‐dependent release of calcitonin gene‐related peptide (CGRP) triggered by endotoxin in rat mesenteric arterial bed

Wang¹,

Wu²,

Tang³

et al. 1996

British J Pharmacology

View full text Add to dashboard Cite

1 Our objective was to determine whether endotoxin (ETX) could directly trigger the release of calcitonin gene-related peptide (CGRP) from perivascular sensory nerves in the isolated mesenteric arterial bed (MAB) of the rat and to determine whether nitric oxide (NO) and prostaglandins (PGs) are involved. 2 ETX caused time-and concentration-dependent release of CGRP, and as much as a 17 fold increase in CGRP levels in the perfusate at 10-15 min after the administration of ETX (50 ,ug ml-'). 3 CGRP-like immunoreactivity in the perfusate was shown to co-elute with synthetic rat CGRP by reverse-phase h.p.l.c. 4 Pretreatment of MAB with capsaicin or ruthenium red inhibited ETX-induced CGRP release by 90% and 71%, respectively. ETX-evoked CGRP release was decreased by 84% during Ca2"-free perfusion.5 The release of CGRP evoked by ETX was enhanced by L-arginine by 43% and inhibited by Nwnitro-L-arginine (L-NOARG) and methylene blue by 37% and 38%, respectively. L-Arginine reversed the effect of L-NOARG. 6 Indomethacin and ibuprofen also inhibited the ETX-induced CGRP release by 34% and 44%, respectively. No additive inhibition could be found when L-NOARG and indomethacin were concomitantly incubated. 7 The data suggest that ETX triggers the release of CGRP from capsaicin-sensitive sensory nerves innervating blood vessels. The ETX-induced CGRP release is dependent on extracellular Ca2" influx and involves a ruthenium red-sensitive mechanism. Both NO and PGs appear to be involved in the ETXinduced release of CGRP in the rat mesenteric arterial bed.

show abstract

“…In many vascular preparations the peptide-induced relaxation occurs in the absence of the endothelium (endothelium-independent) e.g. bovine coronary artery (Greenberg et al, 1987), rat perfused mesentery (Han et al, 1990) and human and cat cerebral arteries (Edvinsson et al, 1985;Marshall, 1989). However, in the rat thoracic aortic ring preparation, this relaxant effect to CGRP is wholly dependent on the presence of an intact endothelium (Brain et al, 1985).…”

Section: Introductionmentioning

confidence: 99%

Human α‐calcitonin gene‐related peptide stimulates adenylate cyclase and guanylate cyclase and relaxes rat thoracic aorta by releasing nitric oxide

Gray

Marshall

1992

British J Pharmacology

139

View full text Add to dashboard Cite

1 The signal transduction pathway for vasorelaxation induced by human a-calcitonin gene-related peptide (human a-CGRP) was studied in rat thoracic aortic rings preconstricted with noradrenaline (10-7 M). 2 Vasorelaxation by human a-CGRP was inhibited by haemogobin (10-6M) and methylene blue (10-5 M) but was unaffected by ibuprofen (10-5 M).3 Acetylcholine caused a 16 fold increase in levels of guanosine 3':5'-cyclic monophosphate (cyclic GMP) with levels of adenosine 3':5'-cyclic monophosphate (cyclic AMP) being unaltered. Human a-CGRP caused a 12 fold increase in levels of cyclic GMP but, in contrast to acetylcholine, evoked a 2.5 fold rise in levels of cyclic AMP. The rises in cyclic nucleotides evoked by human a-CGRP and acetylcholine were dependent on the presence of an intact endothelium. 4 N0-nitro-L-arginine (L-NOARG: 10-5 M), which inhibits nitric oxide synthase, inhibited the relaxant response to human a-CGRP and cyclic GMP accumulation without affecting the cyclic AMP accumulation. 5 The data presented in this paper suggests that human a-CGRP relaxes the rat thoracic aorta by releasing nitric oxide and stimulating guanylate cyclase. The stimulation of adenylate cyclase by human a-CGRP probably precedes the activation of nitric oxide synthase but could be unrelated to the relaxant response.

show abstract

Perivascular peptides relax cerebral arteries concomitant with stimulation of cyclic adenosine monophosphate accumulation or release of an endothelium-derived relaxing factor in the cat

Cited by 362 publications

References 7 publications

Vasorelaxation in isolated bone arteries: Vasoactive intestinal peptide, substance P, calcitonin gene-related peptide bradykinin studied in pigs

Vasorelaxation in isolated bone arteries: Vasoactive intestinal peptide, substance P, calcitonin gene-related peptide bradykinin studied in pigs

Rapid nitric oxide‐ and prostaglandin‐dependent release of calcitonin gene‐related peptide (CGRP) triggered by endotoxin in rat mesenteric arterial bed

Human α‐calcitonin gene‐related peptide stimulates adenylate cyclase and guanylate cyclase and relaxes rat thoracic aorta by releasing nitric oxide

Contact Info

Product

Resources

About