Perivascular Adipose Tissue Oxidative Stress in Obesity

Man, Andy W C; Zhou, Yawen; Xia, Ning; Li, Huige

doi:10.3390/antiox12081595

Cited by 4 publications

(5 citation statements)

References 175 publications

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“…Oxidative stress is a cellular response caused by an imbalance between reactive oxygen species (ROS) production and antioxidant defenses, which can lead to cellular damage and in PLOS ONE some cases cell death [26,27]. Evidence is mounting that human OB is a state of chronic oxidative stress of the whole body since it perturbs the antioxidant defense system and reduces the activity of protective antioxidant enzymes in tissues [28][29][30]. Moreover, the chondrocytes and synovial fibroblasts of OA can also produce large amounts of ROS in response to biomechanical or biochemical stimuli, thus aggravating oxidative stress and cell damage in OA [31][32][33].…”

Section: Plos Onementioning

confidence: 99%

Potential diagnostic markers and biological mechanism for osteoarthritis with obesity based on bioinformatics analysis

Li,

Tang,

2023

PLoS ONE

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Numerous observational studies have shown that obesity (OB) is a significant risk factor in the occurrence and progression of osteoarthritis (OA), but the underlying molecular mechanism between them remains unclear. The study aimed to identify the key genes and pathogeneses for OA with OB. We obtained two OA and two OB datasets from the gene expression omnibus (GEO) database. First, the identification of differentially expressed genes (DEGs), weighted gene co-expression network analysis (WGCNA), and machine learning algorithms were used to identify key genes for diagnosing OA with OB, and then the nomogram and receiver operating characteristic (ROC) curve were conducted to assess the diagnostic value of key genes. Second, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed to explore the pathogenesis of OA with OB. Third, CIBERSORT was created to investigate immunocyte dysregulation in OA and OB. In this study, two genes (SOD2, ZNF24) were finally identified as key genes for OA with OB. These two key genes had high diagnostic values via nomogram and ROC curve calculation. Additionally, functional analysis emphasized that oxidative stress and inflammation response were shared pathogenesis of OB and AD. Finally, in OA and OB, immune infiltration analysis showed that SOD2 closely correlated to M2 macrophages, regulatory T cells, and CD8 T cells, and ZNF24 correlated to regulatory T cells. Overall, our findings might be new biomarkers or potential therapeutic targets for OA and OB comorbidity.

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Section: Plos Onementioning

confidence: 99%

Potential diagnostic markers and biological mechanism for osteoarthritis with obesity based on bioinformatics analysis

Li,

Tang,

2023

PLoS ONE

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“…Recent attention has focused on the physiological impact of the PVAT on vascular physiology [11,[26][27][28][29]. The paradigm of paracrine signaling from the PVAT to the underlying vessel wall is particularly important in the regulation of vasoreactivity and vascular disease.…”

Section: Activation Of Notch In Pvat Leads To Altered Vascular Functionmentioning

confidence: 99%

Notch Signaling Regulates Mouse Perivascular Adipose Tissue Function via Mitochondrial Pathways

Yang,

Harrington

et al. 2023

Genes

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Perivascular adipose tissue (PVAT) regulates vascular function by secreting vasoactive substances. In mice, Notch signaling is activated in the PVAT during diet-induced obesity, and leads to the loss of the thermogenic phenotype and adipocyte whitening due to increased lipid accumulation. We used the Adiponectin-Cre (Adipoq-Cre) strain to activate a ligand-independent Notch1 intracellular domain transgene (N1ICD) to drive constitutive Notch signaling in the adipose tissues (N1ICD;Adipoq-Cre). We previously found that constitutive activation of Notch1 signaling in the PVAT phenocopied the effects of diet-induced obesity. To understand the downstream pathways activated by Notch signaling, we performed a proteomic analysis of the PVAT from control versus N1ICD;Adipoq-Cre mice. This comparison identified prominent changes in the protein signatures related to metabolism, adipocyte homeostasis, mitochondrial function, and ferroptosis. PVAT-derived stromal vascular fraction cells were derived from our mouse strains to study the cellular and molecular phenotypes during adipogenic induction. We found that cells with activated Notch signaling displayed decreased mitochondrial respiration despite similar levels of adipogenesis and mitochondrial number. We observed variable regulation of the proteins related to mitochondrial dynamics and ferroptosis, including PHB3, PINK1, pDRP1, and the phospholipid hydroperoxidase GPX4. Mitochondria regulate some forms of ferroptosis, which is a regulated process of cell death driven by lipid peroxidation. Accordingly, we found that Notch activation promoted lipid peroxidation and ferroptosis in PVAT-derived adipocytes. Because the PVAT phenotype is a regulator of vascular reactivity, we tested the effect of Notch activation in PVAT on vasoreactivity using wire myography. The aortae from the N1ICD;Adipoq-Cre mice had increased vasocontraction and decreased vasorelaxation in a PVAT-dependent and age-dependent manner. Our data provide support for the novel concept that increased Notch signaling in the adipose tissue leads to PVAT whitening, impaired mitochondrial function, increased ferroptosis, and loss of a protective vasodilatory signal. Our study advances our understanding of how Notch signaling in adipocytes affects mitochondrial dynamics, which impacts vascular physiology.

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“…Obesity is characterized by oxidative stress and persistent inflammation [19], which can cause changes in the conformation and native properties of redox-sensitive enzymes of H 2 S metabolism and the development of deficiency of their cofactors and substrates. In particular, zinc, α-LA and thiosulfate are recognized antioxidants, and their need may increase under oxidative stress.…”

Section: клінічна та експериментальна медицина / Clinical and Experim...mentioning

confidence: 99%

“…Ензим (кофактор) Схема реакції Ожиріння характеризується оксидативним стресом та персистуючим запаленням [19], що може викликати зміни конформації та нативних властивостей редокс-чутливих ензимів обміну H 2 S, розвиток дефіциту їх кофакторів та субстратів. Зокрема, цинк, α-ЛК та тіосульфат є визнаними антиоксидантами і за оксидативного стресу потреба в них може зростати.…”

Section: таблиця -основні ензиматичні реакцій обміну н 2 S в серцево-...unclassified

The Effect of Lipoic Acid, Zinc Sulfate and Sodium Thiosulfate on H2 S Metabolism in Cardiovascular System of Rats in Experimental Obesity

Bobetska,

Zaichko

2023

VPBM

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Вісник проблем біології і медицини -2023 -Вип. 4 (171) / Bulletin of problems in biology and medicine -2023 -Issue 4 (171) 134 КЛІНІЧНА ТА ЕКСПЕРИМЕНТАЛЬНА МЕДИЦИНА / CLINICAL AND EXPERIMENTAL MEDICINEMaterials and methods. The research material consisted of the results of examination and treatment of 140 patients diagnosed with varicose veins of the lower extremities at the Istanbul NS Clinic and the Yeni Ganja Medical Center during 2017-2020. Among the patients there were 98 men, 42 women, average age 57.1±0.62 years.The duration of diabetes mellitus was 13.8±0.33 years. At the same time, 98 (70%) patients had a history of diabetes mellitus of 10-14 years, 27 (19.29%) patients -15-19 years, 13 (9.29%) patients -20-24 years, and 2 (1.43%) patients had more than 25 years. The concentration of sugar in the blood was 183.9±4.9 mg/dl, and the amount of glycohemoglobin was 9.0±0.09%, varying from 7.5% to 11.9%.EVLA was performed for all patients after appropriate preoperative preparation. Depending on the energy density, the patients were divided into control and main groups. In 60 patients included in the control group, the speed limit of the extractor device was set at 1 mm/s, and the power was set at 8-10 Watts to supply 80-100 J/cm of energy to the vein. 80 patients of the main group were divided into 2 subgroups of 40 people each.Results and discussion. Varicose veins of the lower extremities are considered the most obvious clinical sign of chronic venous insufficiency. Varicose veins significantly decreased (p<0.0001) in the control group a week after EVLA. Varicose veins were not detected in 57.1% of patients on the right and 25 on the left. In 24 people on the left, the number and volume of varicose veins significantly decreased (p<0.0001) and decreased to the level to be assessed at 1 point.140 patients with diabetes mellitus underwent EVLA. In the control group (n=60), energy was applied in 80-100 J/cm; in the main A subgroup (n=40) 100-120 J/cm and in the main subgroup (n=40) 120-140 J/cm. The results on the VCSS scale were compared a week, 1 month, 6 months and 1 year after EVLA. The analysis proved that EVLA with an energy output of 120-140 J/cm is more effective in patients with diabetes mellitus.In our study, positive effect was confirmed by the best VCSS quality of life indicators and a significant reduction in the number of relapses after surgery in patients with diabetic varicose veins.

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Perivascular Adipose Tissue Oxidative Stress in Obesity

Cited by 4 publications

References 175 publications

Potential diagnostic markers and biological mechanism for osteoarthritis with obesity based on bioinformatics analysis

Potential diagnostic markers and biological mechanism for osteoarthritis with obesity based on bioinformatics analysis

Notch Signaling Regulates Mouse Perivascular Adipose Tissue Function via Mitochondrial Pathways

The Effect of Lipoic Acid, Zinc Sulfate and Sodium Thiosulfate on H2 S Metabolism in Cardiovascular System of Rats in Experimental Obesity

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