Periurethral Injection of Autologous Adipose-Derived Stem Cells with Controlled-Release Nerve Growth Factor for the Treatment of Stress Urinary Incontinence in a Rat Model

Zhao, Weiming; Zhang, Cheng; Jin, Chengjun; Zhang, Zijian; Kong, Deling; Xu, Wanhai; Xiu, Youcheng

doi:10.1016/j.eururo.2010.10.038

Cited by 97 publications

(60 citation statements)

References 28 publications

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“…35,36 In our study, BrdU labeling colocalized with myosin expression within the bundles of smooth muscle after rASCs implantation (Fig. 10A-D).…”

Section: Figsupporting

confidence: 58%

253 Epithelial-Differentiated Adipose-Derived Stem Cells Seeded Bladder Acellular Matrix Grafts for Urethral Reconstruction: An Animal Model

Li¹,

Xu²,

Fei³

et al. 2013

Journal of Urology

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The limited amount of available epithelial tissue is considered a main cause of the high rate of urethral reconstruction failures. The aim of this study was to investigate whether epithelial-differentiated rabbit adiposederived stem cells (Epith-rASCs) could play a role of epithelium in vivo functionally and be a potential substitute of urothelium. Substitution urethroplasty was performed to repair an anterior urethral defect in male New Zealand rabbits using Epith-rASCs seeded bladder acellular matrix grafts (BAMGs) after 5-bromo-2¢-deoxyuridine (BrdU) labeling, based on the in vitro epithelial induction system we previously described. Urethroplasty with cell-free BAMGs and with undifferentiated rASCs (Und-rASCs) seeded BAMGs were performed as controls. After surgery, a notable amelioration of graft contracture and recovery of urethral continuity were observed in the Epith-rASCs/BAMG group by retrograde urethrograms and macroscopic inspection. Immunofluorescence revealed that the BrdU-labeled Epith-rASCs/Und-rASCs colocalized with cytokeratin 13 or myosin. Consistent with the results of western blotting, at early postimplantation stage, the continuous epithelial layer with local multilayered structure was observed in the Epith-rASCs/BAMG group, whereas no significant growth and local monolayer growth profile of epithelial cells were observed in the BAMG and Und-rASCs/BAMG group, respectively. The results showed that Epith-rASCs could serve as a potential substitute of urothelium for urethral tissue engineering and be available to prevent lumen contracture and subsequent complications including recurrent stricture.

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“…35,36 In our study, BrdU labeling colocalized with myosin expression within the bundles of smooth muscle after rASCs implantation (Fig. 10A-D).…”

Section: Figsupporting

confidence: 58%

253 Epithelial-Differentiated Adipose-Derived Stem Cells Seeded Bladder Acellular Matrix Grafts for Urethral Reconstruction: An Animal Model

Li¹,

Xu²,

Fei³

et al. 2013

Journal of Urology

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“…MSCs are currently being investigated in clinical trials for stress urinary incontinence as an i.m. injection probably because the urinary sphincter is much smaller in volume than the anal sphincter complex [5,26,28,47]. Our results therefore have significance for directing future clinical trials of stem cell therapy for fecal incontinence by suggesting that i.m.…”

Section: Discussionmentioning

confidence: 91%

“…Adiposederived stem cells [26][27][28][29], muscle-derived stem cells [30], and mesenchymal stem cells (MSCs) [31] have been used to improve functioning of the injured myocardium and the urinary sphincter in animal models. In the anal sphincter, the effect of MSCs has been studied, although most of the studies have used ex vivo outcome testing [32][33][34][35][36][37].…”

Section: Introductionmentioning

confidence: 99%

Functional Outcome After Anal Sphincter Injury and Treatment With Mesenchymal Stem Cells

Salcedo

Penn

Damaser

et al. 2014

Stem Cells Translational Medicine

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This research demonstrates the regenerative effects of mesenchymal stem cells (MSCs) on the injured anal sphincter by comparing anal sphincter pressures following intramuscular and serial intravascular MSC infusion in a rat model of anal sphincter injury. Fifty rats were divided into injury (n = 35) and no injury (NI; n = 15) groups. Each group was further divided into i.m., serial i.v., or no-treatment (n = 5) groups and followed for 5 weeks. The injury consisted of an excision of 25% of the anal sphincter complex. Twenty-four hours after injury, 5 3 10 5 green fluorescent protein-labeled MSCs in 0.2 ml of phosphate-buffered saline (PBS) or PBS alone (sham) were injected into the anal sphincter for i.m. treatment; i.v. and sham i.v. treatments were delivered daily for 6 consecutive days via the tail vein. Anal pressures were recorded before injury and 10 days and 5 weeks after treatment. Ten days after i.m. MSC treatment, resting and peak pressures were significantly increased compared with those in sham i.m. treatment (p < .001). When compared with the NI group, the injury groups had anal pressures that were not significantly different 5 weeks after i.m./i.v. treatment. Both resting and peak pressures were also significantly increased after i.m./i.v. MSC treatment compared with treatment with PBS (p < .001), suggesting recovery. Statistical analysis was done using paired t test with Bonferroni correction. Marked decrease in fibrosis and scar tissue was seen in both MSC-treated groups. Both i.m. and i.v. MSC treatment after injury caused an increase in anal pressures sustained at 5 weeks, although fewer cells were injected i.m. The MSC-treated groups showed less scarring than the PBS-treated groups, with the i.v. infusion group showing the least scarring. STEM CELLS TRANSLATIONAL MEDICINE 2014;3:760-767

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“…According to their data, animals with ADSC treatment also showed significant myogenic differentiation or regeneration and improved LPP results. ADSCs combined with neuronal growth factor controlled release material might lead to better overall outcomes [72].…”

Section: Stress Urinary Incontinence (Sui)mentioning

confidence: 99%

Potential application of adipose tissue-derived stem cells for urological Disease

Lin

Lue

2014

Bladder

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Adipose tissue-derived stem cells (ADSCs) are a somatic stem cell population contained in fat tissue that may be utilized in the treatment of urologic disease. ADSCs are excellent candidates for these therapies as they are easily obtained in large quantities from adipose tissue, and possess the potential to undergo long-term proliferation, self-renewal and multipotent differentiation. We reviewed the available evidence from 1964 through 2014 concerning ADSC availability, differentiation, and potentiality in the context of treatment for urologic diseases.

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Periurethral Injection of Autologous Adipose-Derived Stem Cells with Controlled-Release Nerve Growth Factor for the Treatment of Stress Urinary Incontinence in a Rat Model

Cited by 97 publications

References 28 publications

253 Epithelial-Differentiated Adipose-Derived Stem Cells Seeded Bladder Acellular Matrix Grafts for Urethral Reconstruction: An Animal Model

253 Epithelial-Differentiated Adipose-Derived Stem Cells Seeded Bladder Acellular Matrix Grafts for Urethral Reconstruction: An Animal Model

Functional Outcome After Anal Sphincter Injury and Treatment With Mesenchymal Stem Cells

Potential application of adipose tissue-derived stem cells for urological Disease

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