Peritoneal Submesothelial Stromal Cells Support Hematopoiesis and Differentiate into Osteogenic and Adipogenic Cell Lineages

Amaral, Rui; Benac, P.; Andrade, Luciana Segura de; Farina, Mirko; Bernardazzi, Cláudio; Arcanjo, Katia; Palumbo, A; Cordeiro, Ingrid Rosenburg; Brito, José M.; El-Cheikh, Márcia Cury; Oliveira, Felipe Leite de

doi:10.1159/000377624

Cited by 7 publications

(6 citation statements)

References 40 publications

(44 reference statements)

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“…MC expresses additional biochemical markers suggesting an active role of these cells in fluid transport, initiation and resolution of inflammation. These biochemical markers include aquaporin 1, sodium-potassium adenosine triphosphatase (Na + /K + -ATPase), sodium bicarbonate cotransporter (SLC) [20], integrin β1 (CD29) [26][27][28], CD40 as a member of the tumor necrosis factor (TNF)-receptor…”

Section: Phenotypic or Biochemical Markers Of MCmentioning

confidence: 99%

Diverse properties of the mesothelial cells in health and disease

Kawanishi¹

2016

Pleura and Peritoneum

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Mesothelial cells (MCs) form the superficial anatomic layer of serosal membranes, including pleura, pericardium, peritoneum, and the tunica of the reproductive organs. MCs produce a protective, non-adhesive barrier against physical and biochemical damages. MCs express a wide range of phenotypic markers, including vimentin and cytokeratins. MCs play key roles in fluid transport and inflammation, as reflected by the modulation of biochemical markers such as transporters, adhesion molecules, cytokines, growth factors, reactive oxygen species and their scavengers. MCs synthesize extracellular matrix related molecules, and the surface of MC microvilli secretes a highly hydrophilic protective barrier, “glycocalyx”, consisting mainly of glycosaminoglycans. MCs maintain a balance between procoagulant and fibrinolytic activation by producing a whole range of regulators, can synthetize fibrin and therefore form adhesions. Synthesis and recognition of hyaluronan and sialic acids might be a new insight to explain immunoactive and immunoregulatory properties of MCs. Epithelial to mesenchymal transition of MCs may involve serosal repair and remodeling. MCs might also play a role in the development and remodeling of visceral adipose tissue. Taken together, MCs play important roles in health and disease in serosal cavities of the body. The mesothelium is not just a membrane and should be considered as an organ.

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Section: Phenotypic or Biochemical Markers Of MCmentioning

confidence: 99%

Diverse properties of the mesothelial cells in health and disease

Kawanishi¹

2016

Pleura and Peritoneum

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“…Mesothelial cells have also been isolated from adherent or free-floating ascites, lavage, and exudate sources and often also cultured; as such, these cells as well as subserosal cells have been shown to possess multiple properties [ 143 , 144 , 145 ]. However, it is not clear whether free-floating cells are the same as adherent cells, if contaminated mesothelial cell populations have been isolated due to lack of additional purification steps or whether selection pressures during culture have influenced the cell population analysed and/or its phenotype.…”

Section: Further Discussion and Areas Of Future Researchmentioning

confidence: 99%

Post-Surgical Peritoneal Scarring and Key Molecular Mechanisms

Herrick

Wilm

2021

Biomolecules

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Post-surgical adhesions are internal scar tissue and a major health and economic burden. Adhesions affect and involve the peritoneal lining of the abdominal cavity, which consists of a continuous mesothelial covering of the cavity wall and majority of internal organs. Our understanding of the full pathophysiology of adhesion formation is limited by the fact that the mechanisms regulating normal serosal repair and regeneration of the mesothelial layer are still being elucidated. Emerging evidence suggests that mesothelial cells do not simply form a passive barrier but perform a wide range of important regulatory functions including maintaining a healthy peritoneal homeostasis as well as orchestrating events leading to normal repair or pathological outcomes following injury. Here, we summarise recent advances in our understanding of serosal repair and adhesion formation with an emphasis on molecular mechanisms and novel gene expression signatures associated with these processes. We discuss changes in mesothelial biomolecular marker expression during peritoneal development, which may help, in part, to explain findings in adults from lineage tracing studies using experimental adhesion models. Lastly, we highlight examples of where local tissue specialisation may determine a particular response of peritoneal cells to injury.

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“…The submesothelial reactive response to tissue damages has been described in distinct models 31–33 . Recently, our group demonstrated that submesothelial cells derived from peritoneal explants have fibroblast-like behavior, mesenchymal phenotype, and stromal functions 34,35 . The pristane-model was marked by important disturbances on oil granuloma formation in Lgals3 −/− mice, including large necrotic areas and high levels of IL-6 7 .…”

Section: Discussionmentioning

confidence: 99%

Galectin-3 orchestrates the histology of mesentery and protects liver during lupus-like syndrome induced by pristane

Lemos

Pereira

Carvalho

et al. 2019

Sci Rep

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Galectin-3 (Gal-3) controls intercellular and cell-extracellular matrix interactions during immunological responses. In chronic inflammation, Gal-3 is associated with fibrotic events, regulates B cell differentiation and delays lupus progression. Gal-3 deficient mice (Lgals3−/−) have intense germinal center formation and atypical plasma cell generation correlated to high levels IgG, IgE, and IgA. Here, we used pristane (2,6,10,14-tetramethylpentadecane) to induce lupus-like syndrome in Lgals3−/− and Lgals3+/+ BALB/c mice. Mesentery and peritoneal cells were monitored because promptly react to pristane injected in the peritoneal cavity. For the first time, mesenteric tissues have been associated to the pathogenesis of experimental lupus-like syndrome. In Lgals3+/+ pristane-induced mice, mesentery was hallmarked by intense fibrogranulomatous reaction restricted to submesothelial regions and organized niches containing macrophages and B lymphocytes and plasma cells. In contrast, Lgals3−/− pristane-treated mice had diffuse mesenteric fibrosis affecting submesothelium and peripheral tissues, atypical M1/M2 macrophage polarization and significant DLL1+ cells expansion, suggesting possible involvement of Notch/Delta pathways in the disease. Early inflammatory reaction to pristane was characterized by significant disturbances on monocyte recruitment, macrophage differentiation and dendritic cell (DC) responses in the peritoneal cavity of pristane-induced Lgals3−/− mice. A correlative analysis showed that mesenteric damages in the absence of Gal-3 were directly associated with severe portal inflammation and hepatitis. In conclusion, it has suggested that Gal-3 orchestrates histological organization in the mesentery and prevents lupoid hepatitis in experimental lupus-like syndrome by controlling macrophage polarization, Notch signaling pathways and DC differentiation in mesenteric structures.

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Peritoneal Submesothelial Stromal Cells Support Hematopoiesis and Differentiate into Osteogenic and Adipogenic Cell Lineages

Cited by 7 publications

References 40 publications

Diverse properties of the mesothelial cells in health and disease

Diverse properties of the mesothelial cells in health and disease

Post-Surgical Peritoneal Scarring and Key Molecular Mechanisms

Galectin-3 orchestrates the histology of mesentery and protects liver during lupus-like syndrome induced by pristane

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