Peritoneal macrophages show increased cytokine gene expression following haemorrhagic shock

Zhu, Xiaodong; Ayala, Alfonsa Garcı́a; Zellweger, René; Mh, Morrison; Ih, Chaudry

doi:10.1016/0300-9572(95)99841-w

Cited by 10 publications

(9 citation statements)

References 2 publications

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“…In contrast, proinflammatory cytokine RNA expression in splenic and peritoneal macrophages was increased following trauma and blood loss after 2 h of LPS stimulation (35,36). Thus an increased activation of p38 in splenic and peritoneal macrophages of males following traumahemorrhage might result in an increased inflammatory cytokine response that is associated with an exhaustion of the capacity of those cells to release proinflammatory cytokines in vitro.…”

Section: Discussionmentioning

confidence: 93%

Sex-specific p38 MAP kinase activation following trauma-hemorrhage: involvement of testosterone and estradiol

Angele

Nitsch²,

Knöferl³

et al. 2003

American Journal of Physiology-Endocrinology and Metabolism

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. Sex-specific p38 MAP kinase activation following trauma-hemorrhage: involvement of testosterone and estradiol. Am J Physiol Endocrinol Metab 285: E189-E196, 2003; 10.1152/ajpendo.00035. 2003.-Although immune functions are markedly depressed in males and not in proestrous females following traumahemorrhage (T-H), the mechanisms responsible for the divergent responses remain unknown. Because sex steroids modulate the activation of p38, our aim was to determine whether differences in the activation of p38 by phosphorylation (p38-P) might contribute to the sex-dimorphic immune response following T-H. The effects of testosterone and estradiol on the activation of p38 were also examined. Intact male mice (C3H/HeN), castrated males treated with vehicle, 5␣-dihydrotestosterone (DHT), or 17␤-estradiol, and proestrous females were subjected to trauma (i.e., midline laparotomy) and hemorrhagic shock (35 Ϯ 5 mmHg for 90 min and resuscitation) or sham operation. At 2 h thereafter, splenic (SM) and peritoneal macrophages (PM) were harvested and cultured (with 10 g/ml LPS), and Western blot analysis was carried out for quantification of p38 and p38-P. Sex, testosterone and estradiol plasma levels, and T-H did not alter the constitutive expression of p38 in SM and PM. In contrast, the activated form of p38 (p38-P) was markedly increased in SM and PM from female shams compared with male shams. Moreover, the phosphorylation of p38-P increased in males after T-H, whereas it decreased in females under those conditions. Castration before T-H prevented the increase in p38-P in males. Castrated animals treated with DHT displayed increased p38-P following T-H, whereas 17␤-estradiol had no effect on p38-P in castrated mice. Thus 1) sex influences the activation of p38 MAP kinase, 2) DHT is responsible for the increased activation of p38 in male mice, and 3) this sex-specific activation of p38 might be responsible for the sexually dimorphic immune response following T-H.

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Section: Discussionmentioning

confidence: 93%

Sex-specific p38 MAP kinase activation following trauma-hemorrhage: involvement of testosterone and estradiol

Angele

Nitsch²,

Knöferl³

et al. 2003

American Journal of Physiology-Endocrinology and Metabolism

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“…A reduced production of IL-1␤, IL-6, and TNF-␣ is known after hemorrhagic shock, with or without an associated midline laparotomy (29,43). However, on a transcriptional level, a significant increase in IL-1␤, IL-6, and TNF-␣ was noticed, suggesting an important influence of posttranscriptional regulatory mechanisms in peritoneal macrophages after hemorrhage (44). Polymicrobial sepsis has been found to reduce the capacity of peritoneal macrophages to release proinflammatory cytokines in re- sponse to lipopolysaccharide (45).…”

Section: Discussionmentioning

confidence: 98%

Pulmonary contusion causes impairment of macrophage and lymphocyte immune functions and increases mortality associated with a subsequent septic challenge*

Perl

Gebhard²,

Brückner³

et al. 2005

Critical Care Medicine

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“…Resident peritoneal macrophages (pMf) were obtained following the protocol of Ayala et al [18], and pMf monolayers were established as described previously [19]. The monolayers were incubated with 50 ng lipopolysaccharide (derived from Salmonella friedenau) for 48 h at 37°C, 5% CO 2 and 90% humidity.…”

Section: Preparation Of Macrophage Culturementioning

confidence: 99%

Inhibition of Cyclooxygenase-2 by NS-398 Following Hemorrhage and Subsequent Sepsis: No Beneficial Effects in Either Gender

Kahlke

Seidel

Stapela

et al. 2004

Surgical Infections

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Peritoneal macrophages show increased cytokine gene expression following haemorrhagic shock

Cited by 10 publications

References 2 publications

Sex-specific p38 MAP kinase activation following trauma-hemorrhage: involvement of testosterone and estradiol

Sex-specific p38 MAP kinase activation following trauma-hemorrhage: involvement of testosterone and estradiol

Pulmonary contusion causes impairment of macrophage and lymphocyte immune functions and increases mortality associated with a subsequent septic challenge*

Inhibition of Cyclooxygenase-2 by NS-398 Following Hemorrhage and Subsequent Sepsis: No Beneficial Effects in Either Gender

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