Peritoneal adhesion prevention by a biodegradable hyaluronic acid-based hydrogel formed in situ through a cascade enzyme reaction initiated by contact with body fluid on tissue surfaces

Sakai, Shinji; Ueda, Kohei; Taya, Masahito

doi:10.1016/j.actbio.2015.06.023

Cited by 51 publications

(39 citation statements)

References 30 publications

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“…They then applied it to rats with damaged abdominal walls to successfully suppress peritoneal adhesion. In one report, an HA–Ph hydrogel cross‐linked through HRP‐mediated cross‐linking effectively prevented peritoneal adhesion by combining with the indirect H 2 O 2 supply as a result of the use of GOx . The solution, containing HA–Ph, HRP, and GOx, injected into the peritoneal cavity of rats with damaged abdominal walls formed a hydrogel on tissue surfaces and prevented adhesion (Figure ).…”

Section: Biomedical and Biopharmaceutical Applicationsmentioning

confidence: 99%

Horseradish Peroxidase Catalyzed Hydrogelation for Biomedical, Biopharmaceutical, and Biofabrication Applications

Sakai

Nakahata

2017

Chemistry An Asian Journal

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Horseradish peroxidase (HRP)-catalyzed hydrogelation has attracted much attention owing to the ease of handling, high biocompatibility, and processability. This review summarizes recent developments, including cutting-edge research into the use of HRP-mediated hydrogelation toward biomedical, biopharmaceutical, and biofabrication applications. From the viewpoint of polymer chemistry, the basic chemistry behind hydrogelation, the structure-property relationship, and hybridization of multiple materials by using HRP-catalyzed hydrogelation are summarized. From the chemical engineering perspective, strategies for controlling hydrogelation kinetics, hydrogel characteristics, and hydrogelation processes are summarized and discussed in detail. Strategies for obtaining biomaterials for medical and pharmaceutical use, and biofabrication for tissue engineering and regenerative medicine emerge by unifying the aspects of HRP-mediated hydrogelation.

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Section: Biomedical and Biopharmaceutical Applicationsmentioning

confidence: 99%

Horseradish Peroxidase Catalyzed Hydrogelation for Biomedical, Biopharmaceutical, and Biofabrication Applications

Sakai

Nakahata

2017

Chemistry An Asian Journal

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“…Many researchers and companies have focused on the development of injectable hydrogels for different kinds of tissues (Barbucci et al, 2002;Sakai et al, 2015;Varma et al, 2014;Su et al, 2010). Injectable hydrogels can be maintained in the liquid state before injection and harden in situ after transplantation in vivo.…”

Section: Mh Hu Et Almentioning

confidence: 99%

“…Using a light microscope, each section was evaluated and scored for the extent of fibrosis, dura adhesion and density of fibroblasts. The dura adhesion parameters were evaluated according to the criteria proposed by He and colleagues, already proved to have good inter-rater reliability (He et al, 1995;Sae-Jung et al, 2015). Fibroblast cell density was calculated in each field at 40× magnification, following the classification system proposed by Hinton et al (2015), according to which the definition of grade 1 was fewer than 100 fibroblasts in each field, grade 2 100-150 fibroblasts in each field and grade 3 more than 150 fibroblasts in each field.…”

Section: Histopathological Examinationmentioning

confidence: 99%

In situ forming oxidised hyaluronic acid/adipic acid dihydrazide hydrogel for prevention of epidural fibrosis after laminectomy

Yang²,

Sun

et al. 2017

eCM

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Post-operative epidural fibrosis is a biological response after laminectomy that may lead to clinical symptoms, such as radicular pain. An ideal material for prevention of epidural fibrosis should be able to inhibit fibroblast adhesions and reduce formation of scar tissue. An injectable hydrogel would be the material of choice for this purpose, since it could fill an irregular surgical defect completely, gelate in situ and be delivered in a minimally-invasive manner. The objective of this study was to evaluate, in vitro and in vivo, the cytocompatibility and anti-adhesive effect of an oxidised hyaluronic acid/adipic acid dihydrazide (oxi-HA/ADH) hydrogel. Different cell types present in the spine were used to test the cytocompatibility of the hydrogel. The hydrogel extraction medium had no deleterious effects on neural cells (PC-12), but reduced fibroblasts viability (NIH/3T3). Although the hydrogel did not change the release of lactate dehydrogenase from myoblasts (C2C12) and Schwann cells (RSC96), the extraction medium concentration slightly affected the mitochondrial activity of these two cell types. qPCR showed that the hydrogel down-regulated S100a and P4hb expression in NIH/3T3 cells, supporting the hypothesis that the hydrogel might inhibit fibroblast activity. The animal study showed a reduction of scar tissue formation as well as severity of adhesion between scar tissue and the dura mater in a rat laminectomy model. Superficially, the peel-off test showed significantly decreased tenacity. In conclusion, the oxi-HA/ADH hydrogel is a promising injectable and thermosensitive material for prevention of post-operative epidural fibrosis.

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“…1‐Ethyl‐3‐(3‐dimethylaminopropyl) carbodiimide (EDC) was obtained from the Peptide Institute (Osaka, Japan). HA‐Ph (2.3 × 10 −4 mol‐Ph/g) and Gelatin‐Ph (1.6 × 10 −5 mol‐Ph/g) were obtained via condensation of carboxyl moieties of these polymers and amino moieties of tyramine using EDC and NHS according to previous reports . hADSCs were purchased from Lonza (Walkersville, MD) and cultured in subculture medium for ADSCs (PT‐4505, Lonza) containing 10% (v/v) fetal bovine serum.…”

Section: Methodsmentioning

confidence: 99%

Differentiation potential of human adipose stem cells bioprinted with hyaluronic acid/gelatin‐based bioink through microextrusion and visible light‐initiated crosslinking

et al. 2017

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Bioprinting has a great potential to fabricate three-dimensional (3D) functional tissues and organs. In particular, the technique enables fabrication of 3D constructs containing stem cells while maintaining cell proliferation and differentiation abilities, which is believed to be promising in the fields of tissue engineering and regenerative medicine. We aimed to demonstrate the utility of the bioprinting technique to create hydrogel constructs consisting of hyaluronic acid (HA) and gelatin derivatives through irradiation by visible light to fabricate 3D constructs containing human adipose stem cells (hADSCs). The hydrogel was obtained from a solution of HA and gelatin derivatives possessing phenolic hydroxyl moieties in the presence of ruthenium(II) tris-bipyridyl dication and sodium ammonium persulfate. hADSCs enclosed in the bioprinted hydrogel construct elongated and proliferated in the hydrogel. In addition, their differentiation potential was confirmed by examining the expression of pluripotency marker genes and cell surface marker proteins, and differentiation to adipocytes in adipogenic differentiation medium. Our results demonstrate the great potential of the bioprinting method and the resultant hADSC-laden HA/gelatin constructs for applications in tissue engineering and regenerative medicine.

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Peritoneal adhesion prevention by a biodegradable hyaluronic acid-based hydrogel formed in situ through a cascade enzyme reaction initiated by contact with body fluid on tissue surfaces

Cited by 51 publications

References 30 publications

Horseradish Peroxidase Catalyzed Hydrogelation for Biomedical, Biopharmaceutical, and Biofabrication Applications

Horseradish Peroxidase Catalyzed Hydrogelation for Biomedical, Biopharmaceutical, and Biofabrication Applications

In situ forming oxidised hyaluronic acid/adipic acid dihydrazide hydrogel for prevention of epidural fibrosis after laminectomy

Differentiation potential of human adipose stem cells bioprinted with hyaluronic acid/gelatin‐based bioink through microextrusion and visible light‐initiated crosslinking

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