Periplasmic and intracytoplasmic polyphosphate and easily washable phosphate in pure cultures of sewage bacteria

Streichan, Marlies; Schön, G.

doi:10.1016/0043-1354(91)90092-5

Cited by 26 publications

(2 citation statements)

References 12 publications

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“…Earlier data convincingly showed that P in EBPR biomass is organized as intracellular polymeric polyP and is not chemically precipitated on the surface of cells as inorganic deposits or associated with extracellular polymeric substances associated with flocs [64]. Its organization inside PAO is less certain, with various claims supporting its location in the cytoplasm, the periplasm or associated with intracellular membranes [65–67], and possibly complexed with proteins and DNA and RNA [18,61]. These may reflect stages in its synthesis, but all may be possible if PAO contain several different populations, as now seems likely (see ), and each stores polyP differently.…”

Section: The Nature and Role Of Polyp Storagementioning

confidence: 99%

The microbiology of biological phosphorus removal in activated sludge systems

2003

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Activated sludge systems are designed and operated globally to remove phosphorus microbiologically, a process called enhanced biological phosphorus removal (EBPR). Yet little is still known about the ecology of EBPR processes, the microbes involved, their functions there and the possible reasons why they often perform unreliably. The application of rRNA-based methods to analyze EBPR community structure has changed dramatically our understanding of the microbial populations responsible for EBPR, but many substantial gaps in our knowledge of the population dynamics of EBPR and its underlying mechanisms remain. This review critically examines what we once thought we knew about the microbial ecology of EBPR, what we think we now know, and what still needs to be elucidated before these processes can be operated and controlled more reliably than is currently possible. It looks at the history of EBPR, the currently available biochemical models, the structure of the microbial communities found in EBPR systems, possible identities of the bacteria responsible, and the evidence why these systems might operate suboptimally. The review stresses the need to extend what have been predominantly laboratory-based studies to full-scale operating plants. It aims to encourage microbiologists and process engineers to collaborate more closely and to bring an interdisciplinary approach to bear on this complex ecosystem.

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Section: The Nature and Role Of Polyp Storagementioning

confidence: 99%

The microbiology of biological phosphorus removal in activated sludge systems

2003

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“…Again, it should be pointed out that the overall process, often simply described as 'biological' phosphate removal from wastewater, is a complex combination of enzyme-mediated biological mechanisms (as reported here and elsewhere [1,4,12,22]), chemical mechanisms including phosphate adsorption at the outer membrane structures [4,20] of microorganisms and phosphate precipitation [1,2]. The exact 'biological' proportion is still to be investigated.…”

Section: Polyphosphate-dependent Enzyme Activitiesmentioning

confidence: 99%

Polyphosphate-dependent enzymes in some coryneform bacteria isolated from sewage sludge

Bark

Kämpfer

Sponner

et al. 1993

FEMS Microbiology Letters

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Eleven isolates obtained from a laboratory sewage treatment plant, most of them presumptively assigned to the coryneform genera Curtobacterium and Aureobacterium were studied for the presence of intracellular polyphosphates and polyphosphate dependent enzymes. All isolates stored polyphosphates and showed adenylate kinase activities ranging from 64 to 815 mU mg -1. Polyphosphate:AMP phosphotransferase could only be detected in one isolate. Three isolates showed a polyphosphate kinase activity also in minor amounts from 15 to 17 mU mg -1. A polyphosphate dependent NAD or 3-phosphoglycerate kinase could not be detected. Polyphosphate glucokinase activity was measured in cell-free extracts of nine isolates ranging from 2 to 376 mU mg-1. Three isolates showed in addition to the polyphosphate glucokinase, a glucose-6-phosphate-dependent NAD kinase. For the regeneration of NADP from NAD and polyphosphate, this enzyme system may give the isolates a distinct competitive advantage, especially for anabolic processes. The polyphosphate-dependent enzymes reported here may play an additional role in the complex process of 'biological' phosphate removal from wastewater.

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Inorganic Polyphosphates

Kortstee¹,

Loosdrecht²

2002

Biopolymers Online

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Introduction Historical Outline Polyphosphate Physical and Chemical Properties Detection Methods Extraction and Separation Analysis Natural Occurrence Prokaryotes Lower Eukaryotes Higher Animals and Man Biosynthesis Prokaryotic Polyphosphate Kinase (PPK; EC 2.7.4.1) The Prokaryotic Gene ppk Polyphosphate Synthesis in Eukaryotes Biodegradation Prokaryotes Lower Eukaryotes Higher Animals and Man Biological Functions of Polyphosphate Energy Source Acquisition of Competence Calcium Channel Ca 2+ ‐ATPase Divalent Cations A Buffer against Alkali Ions ATP Substitute in Kinase Reactions Regulator of Stress Response Involvement in Gene Expression Virulence, Biofilm Development and Quorum Sensing Applications ATP Regeneration Enhanced Biological Phosphorus Removal (EBPR) Antibacterial Action Outlook and Perspectives Patents

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Periplasmic and intracytoplasmic polyphosphate and easily washable phosphate in pure cultures of sewage bacteria

Cited by 26 publications

References 12 publications

The microbiology of biological phosphorus removal in activated sludge systems

The microbiology of biological phosphorus removal in activated sludge systems

Polyphosphate-dependent enzymes in some coryneform bacteria isolated from sewage sludge

Inorganic Polyphosphates

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