Peripherin- and CGRP-immunoreactive nerve fibers in rat molars have different locations and developmental timing

Veerayutthwilai, Orapin; Luis, Nadyne A.; Crumpton, Rosa; MacDonald, Glen; Byers, Margaret R.

doi:10.1016/j.archoralbio.2006.03.011

Cited by 8 publications

(8 citation statements)

References 39 publications

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“…(1, 2, 3) 200 µm, (a-x) 20 µm (Corpron and Avery 1973;Fristad et al 1994;Veerayutthwilai et al 2006). In the present study, an immunopositive reaction with an anti-NF antibody beneath the odontoblast layer was observed in dental pulp at PN12.…”

Section: Discussionsupporting

confidence: 57%

Developmental studies on the acquisition of perception conducting pathways via TRP channels in rat molar odontoblasts using immunohistochemistry and RT-qPCR

et al. 2019

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Odontoblasts act as dentin formation and sensory receptors. Recently, it was reported that transient receptor potential ankyrin (TRPA) 1, TRP vanilloid (TRPV) 4 and pannexin 1 (PANX-1) play important roles in odontoblast sensory reception. However, it is not known when odontoblasts begin to possess a sense reception function. The aim of this study was to clarify the development of odontoblasts as sense receptors. Sections of mandibular first molars from postnatal day (PN) 0 to PN12 Wistar rats were prepared for hematoxylin-eosin staining. Immunohistochemically, we used anti-dentin sialoprotein (DSP), anti-TRPA1, anti-TRPV4, anti-PANX-1, and anti-neurofilament (NF) antibodies. In addition, we investigated TRPA1 and TRPV4 expression by reverse transcriptional quantitative polymerase chain reaction (RT-qPCR). At PN0, undifferentiated odontoblasts showed no immunoreaction to anti-DSP, anti-TRPA1, anti-TRPV4, or anti-PANX-1 antibodies. However, immunopositive reactions of these antibodies increased during odontoblast differentiation at PN3 and PN6. An immunopositive reaction of the anti-NF antibody appeared in the odontoblast neighborhood at PN12, when the odontoblasts began to form root dentin, and this appeared later than that of the other antibodies. By RT-qPCR, expression of TRPA1 at PN6 was significantly lower than that at PN0 (p < 0.05) and PN3 (p < 0.01). Expression of TRPV4 at PN6 was significantly lower than that at PN0 (p < 0.01) and PN3 (p < 0.01). The results of this study suggest that odontoblasts may acquire sensory receptor function after beginning to form root dentin, when TRPA1, TRPV4, PANX-1 channels, and nerve fibers are completely formed.

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Section: Discussionsupporting

confidence: 57%

Developmental studies on the acquisition of perception conducting pathways via TRP channels in rat molar odontoblasts using immunohistochemistry and RT-qPCR

et al. 2019

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“…No where is this more evident than in the densely innervated mouse molars. Indeed, teeth are advantageous in this regard because of the anatomical segregation of functionally distinct fiber types (Jyvasjarvi and Kniffki, 1987;Byers and Narhi, 2002;Veerayutthwilai et al, 2006). We found Trpm8 GFP to label superficial intradental A␦-fibers, as well as pulpal C-fibers.…”

Section: Discussionmentioning

confidence: 67%

“…We also compared tooth regions innervated by TRPM8 fibers to both CGRP-and peripherin-positive fibers. In the rat, nerve endings of CGRP-fibers are asymmetrically distributed in both the pulp and dentin, with some axons crossing the odontoblast layer demarcating the pulp-dentin border (Byers and Narhi, 2002;Veerayutthwilai et al, 2006). In Trpm8 GFP mouse molars, we observed GFP ϩ axons to cross the odontoblast layer in a manner similar to CGRP ϩ fibers (Fig.…”

Section: Trpm8 Is Expressed In Both Nociceptive and Non-nociceptive Smentioning

confidence: 78%

“…5d, arrowheads). These axons extended toward the odontoblast layer but then turned away, thereby being restricted to the pulp (Veerayutthwilai et al, 2006), whereas GFP axons extended through this layer and entered the dentin (Fig. 5d, arrows).…”

Section: Trpm8 Is Expressed In Both Nociceptive and Non-nociceptive Smentioning

confidence: 99%

“…4c,f ), and that peripherin ϩ C-fibers are restricted to tooth pulp ( Fig. 5d) (Veerayutthwilai et al, 2006). Thus, we examined GFP ϩ axons for peripherinimmunoreactivity in the tooth pulp to determine whether these are indeed C-fibers.…”

Section: Trpm8 Is Expressed In Both Nociceptive and Non-nociceptive Smentioning

confidence: 99%

See 2 more Smart Citations

Diversity in the Neural Circuitry of Cold Sensing Revealed by Genetic Axonal Labeling of Transient Receptor Potential Melastatin 8 Neurons

Takashima¹,

Daniels²,

Knowlton³

et al. 2007

J. Neurosci.

192

214

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Sensory nerves detect an extensive array of somatosensory stimuli, including environmental temperatures. Despite activating only a small cohort of sensory neurons, cold temperatures generate a variety of distinct sensations that range from pleasantly cool to painfully aching, prickling, and burning. Psychophysical and functional data show that cold responses are mediated by both C-and A␦-fibers with separate peripheral receptive zones, each of which likely provides one or more of these distinct cold sensations. With this diversity in the neural basis for cold, it is remarkable that the majority of cold responses in vivo are dependent on the cold and menthol receptor transient receptor potential melastatin 8 (TRPM8). TRPM8-null mice are deficient in temperature discrimination, detection of noxious cold temperatures, injury-evoked hypersensitivity to cold, and nocifensive responses to cooling compounds. To determine how TRPM8 plays such a critical yet diverse role in cold signaling, we generated mice expressing a genetically encoded axonal tracer in TRPM8 neurons. Based on tracer expression, we show that TRPM8 neurons bear the neurochemical hallmarks of both C-and A␦-fibers, and presumptive nociceptors and non-nociceptors. More strikingly, TRPM8 axons diffusely innervate the skin and oral cavity, terminating in peripheral zones that contain nerve endings mediating distinct perceptions of innocuous cool, noxious cold, and first-and second-cold pain. These results further demonstrate that the peripheral neural circuitry of cold sensing is cellularly and anatomically complex, yet suggests that cold fibers, caused by the diverse neuronal context of TRPM8 expression, use a single molecular sensor to convey a wide range of cold sensations.

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Sema3A chemorepellant regulates the timing and patterning of dental nerves during development of incisor tooth germ

et al. 2014

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Semaphorin 3A (Sema3A) axon repellant serves multiple developmental functions. Sema3A mRNAs are expressed in epithelial and mesenchymal components of the developing incisor in a dynamic manner. Here, we investigate the functions of Sema3A during development of incisors using Sema3A-deficient mice. We analyze histomorphogenesis and innervation of mandibular incisors using immunohistochemistry as well as computed tomography and thick tissue confocal imaging. Whereas no apparent disturbances in histomorphogenesis or hard tissue formation of Sema3A (-/-) incisors were observed, nerve fibers were prematurely seen in the presumptive dental mesenchyme of the bud stage Sema3A (-/-) tooth germ. Later, nerves were ectopically present in the Sema3A (-/-) dental papilla mesenchyme during the cap and bell stages, whereas in the Sema3A (+/+) mice the first nerve fibers were seen in the pulp after the onset of dental hard tissue formation. However, no apparent topographic differences in innervation pattern or nerve fasciculation were seen inside the pulp between postnatal and adult Sema3A (+/+) or Sema3A (-/-) incisors. In contrast, an abnormally large number of nerves and arborizations were observed in the Sema3A (-/-) developing dental follicle target field and periodontium and, unlike in the wild-type mice, nerve fibers were abundant in the labial periodontium. Of note, the observed defects appeared to be mostly corrected in the adult incisors. The expressions of Ngf and Gdnf neurotrophins and their receptors were not altered in the Sema3A (-/-) postnatal incisor or trigeminal ganglion, respectively. Thus, Sema3A is an essential, locally produced chemorepellant, which by creating mesenchymal exclusion areas, regulates the timing and patterning of the dental nerves during the development of incisor tooth germ.

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Peripherin- and CGRP-immunoreactive nerve fibers in rat molars have different locations and developmental timing

Cited by 8 publications

References 39 publications

Developmental studies on the acquisition of perception conducting pathways via TRP channels in rat molar odontoblasts using immunohistochemistry and RT-qPCR

Developmental studies on the acquisition of perception conducting pathways via TRP channels in rat molar odontoblasts using immunohistochemistry and RT-qPCR

Diversity in the Neural Circuitry of Cold Sensing Revealed by Genetic Axonal Labeling of Transient Receptor Potential Melastatin 8 Neurons

Sema3A chemorepellant regulates the timing and patterning of dental nerves during development of incisor tooth germ

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