Peripheral T cell lymphoma: value of bone marrow trephine immunophenotyping.

Abstract

SUMMARY Bone marrow infiltrates taken from 11 patients with peripheral T cell lymphoma were immunophenotyped as T cell lymphoma using monoclonal antibodies on frozen bone marrow trephine biopsy specimens. In nine these were taken at diagnosis and in two after failure of treatment to eradicate lymphoma in the marrow. Patterns of infiltration were as follows: diffuse (n = 4), interstitial (n = 1), nodular (n = 1), focal (n = 5). All cases were CD3 positive and 10 were CD2 positive; five lacked expression ofeither CD5 or CD7, or both markers. In nine the determination ofT cell phenotype depended on analysis of the frozen bone marrow trephine biopsy specimen as there was no other biopsy tissue available for study. In the other two cases there was agreement between the immunophenotypes seen in lymph node and bone marrow infiltrates.

Intrasinusoidal bone marrow infiltration: a possible hallmark of splenic lymphoma

“…In such cases immunohistochemistry can provide useful information 19;20 . Furthermore, bone marrow immunohistology is considered crucial in NHL staging procedures 21 and in the definition of T-cell lymphomas 22 .…”

Bone marrow histopathology in peripheral T‐cell lymphomas

“…Immunohistochemistry is very helpful in identifying subtle infiltrates and should be performed when possible (White et al , 1989; Gaulard et al , 1991). The phenotype of the bone marrow infiltrate is often identical to the extramedullary tumour.…”