Peripheral nuclear matrix actin forms perinuclear shells

Clubb, Bryan H.; Locke, Michael

doi:10.1002/(sici)1097-4644(19980801)70:2<240::aid-jcb10>3.0.co;2-r

Cited by 21 publications

(12 citation statements)

References 54 publications

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“…We previously confirmed the specificity of IQGAP1 polyclonal antibody H-109 (Santa Cruz Biotech, CA) by siRNA-mediated knockdown, and showed that this antibody detects similar endogenous IQGAP1 staining patterns to that of ectopically-expressed GFP-tagged IQGAP1 21 . A careful analysis of IQGAP1 subcellular localization with this antibody has revealed a ‘nuclear halo’ pattern of IQGAP1 by immunofluorescence microscopy, reminiscent of that previously reported for actin in interphase NIH 3T3 cells 25 . IQGAP1 is inextricably linked to F-actin, 14 therefore we co-stained for F-actin with FITC-conjugated phalloidin to determine the degree of co-localization.…”

Section: Resultssupporting

confidence: 78%

The scaffolding protein IQGAP1 co-localizes with actin at the cytoplasmic face of the nuclear envelope: implications for cytoskeletal regulation

Johnson

Henderson

2012

BioArchitecture

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IQGAP1 is an important cytoskeletal regulator, known to act at the plasma membrane to bundle and cap actin filaments, and to tether the cortical actin meshwork to microtubules via plus-end binding proteins. Here we describe the novel subcellular localization of IQGAP1 at the cytoplasmic face of the nuclear envelope, where it co-located with F-actin. The IQGAP1 and F-actin staining overlapped that of microtubules at the nuclear envelope, revealing a pattern strikingly similar to that observed at the plasma membrane. In detergent-extracted cells IQGAP1 was retained at cytoskeletal structures at the nuclear envelope. This finding has new implications for involvement of IQGAP1 in cell polarization and migration events and potentially in cell cycle-associated nuclear envelope assembly/disassembly.

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Section: Resultssupporting

confidence: 78%

The scaffolding protein IQGAP1 co-localizes with actin at the cytoplasmic face of the nuclear envelope: implications for cytoskeletal regulation

Johnson

Henderson

2012

BioArchitecture

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“…The studies of nuclear actin (reviewed in Pederson 2000) remind us that filament-forming protein families are present in cells. We can also hypothesize that plasmid DNA might tether to short nuclear actin filaments where actin is partly a component of nuclear matrix, plays a direct part in the nuclear export of retroviral RNA and cellular proteins and is necessary for transcription by RNA polymerase II (Clubb & Locke 1998, Kimura et al 2000, Hofmann et al 2004. Moreover, we can speculate that the plasmid DNA movement to the transcriptionally silent regions represents some kind of cell defence mechanism against alien genetic information.…”

Section: Discussionmentioning

confidence: 99%

Directional motion of foreign plasmid DNA to nuclear HP1 foci

et al. 2006

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Movement of labelled plasmid DNA relative to heterochromatin foci in nuclei, visualized with HP1-GFP, was studied using live-cell imaging and object tracking. In addition to Brownian motion of plasmid DNA we found a pronounced, non-random movement of plasmid DNA towards the nearest HP1 focus, while time-lapse microscopy showed that HP1 foci are relatively immobile and positionally stable. The movement of plasmid DNA was much faster than that of the HP1 foci. Contact of transgene DNA with an HP1 focus usually resulted in cessation of the directional motion. Moreover, the motion of plasmid DNA inside the heterochromatin compartment was more restricted (limited to 0.25 microm) than when the plasmid DNA was outside heterochromatin (R = 0.7 microm). Three days after transfection most of the foreign labelled DNA colocalized with centromeric heterochromatin.

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“…Preparation of Nuclear Matrix and Immunocytochemistry. Nuclear matrix was prepared using the method described by Clubb and Locke (46). Cells were cultured on glass coverslips, and soluble proteins then were extracted by incubation for 1.…”

Section: Methodsmentioning

confidence: 99%

Nuclear Accumulation of Globular Actin as a Cellular Senescence Marker

et al. 2004

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We evaluated the nuclear actin accumulation as a new marker of cellular senescence, using human diploid fibroblast (HDF), chondrocyte primary cultures, Mv1Lu epithelial cells, and Huh7 cancer cells. Nuclear accumulation of globular actin (G-actin) and dephosphorylated cofilin was highly significant in the senescent HDF cells, accompanied with inhibition of LIM kinase (LIMK) -1 activity. When nuclear export of the actin was induced by 12-O-tetradecanoylphorbol-13-acetate, DNA synthesis of the senescent cells increased significantly, accompanied with changes of morphologic and biochemical profiles, such as increased RB protein phosphorylation and decreased expressions of p21 WAF1 , cytoplasmic pextracellular signal-regulated kinase 1/2, and caveolins 1 and 2. Significance of these findings was strengthened additionally by the fact that nuclear actin export of young HDF cells was inhibited by the treatment with leptomycin B and mutant cofilin transfection, whose LIMK-1 phosphorylation site was lost, and the old cell phenotypes were duplicated with nuclear actin accumulation, suggesting that nuclear actin accumulation was accompanied with G1 arrest during cellular senescence. The aforementioned changes were observed not only in the replicative senescence but also in the senescence induced by treatment of HDF cells, Mv1Lu, primary culture of human chondrocytes, or Huh7 cells with H-ras virus infection, hydroxyurea, deferoxamine, or H 2 O 2 . Nuclear actin accumulation was much more sensitive and an earlier event than the well-known, senescence-associated ␤-galactosidase activity.

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Peripheral nuclear matrix actin forms perinuclear shells

Cited by 21 publications

References 54 publications

The scaffolding protein IQGAP1 co-localizes with actin at the cytoplasmic face of the nuclear envelope: implications for cytoskeletal regulation

The scaffolding protein IQGAP1 co-localizes with actin at the cytoplasmic face of the nuclear envelope: implications for cytoskeletal regulation

Directional motion of foreign plasmid DNA to nuclear HP1 foci

Nuclear Accumulation of Globular Actin as a Cellular Senescence Marker

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