Peripheral nerve repair across a gap studied by repeated observation in a new window implant chamber

Humphrey, M.F.; Levy, Walter J.; Dietrich, W. Dalton; Rumpf, R.; Mora, José Alfredo Espinosa

doi:10.1016/0006-8993(89)90978-5

Cited by 11 publications

(4 citation statements)

References 22 publications

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“…From the animal study, in this study, it was observed that the asymmetrically porous PLGA/Pluronic F127 tube‐implanted group applied with low‐intensity pulsed ultrasound had more rapid nerve regeneration behavior (∼0.71 mm/day) than the tube‐implanted group without the ultrasound treatment (∼0.48 mm/day) (Figure 3). Humphrey et al reported that the growth rate of rat sciatic nerve (proximal stump) was about 0.32 mm/day 35. The faster nerve growth rate through the hydrophilized PLGA/Pluronic F127 tube may be owing to its porous structure with high permeability, which plays an important role for nerve regeneration, particularly in the early stages 36.…”

Section: Discussionmentioning

confidence: 99%

“…Humphrey et al reported that the growth rate of rat sciatic nerve (proximal stump) was about 0.32 mm/day. 35 The faster nerve growth rate through the hydrophilized PLGA/ Pluronic F127 tube may be owing to its porous structure with high permeability, which plays an important role for nerve regeneration, particularly in the early stages. 36 In our previous study using the silicone tube, 19 which has been popularly used clinically as a NGC, we could not observe the regenerated nerve at all at the middle section of the tube after 8 weeks implantation even in the same animal model used in this study.…”

Section: Discussionmentioning

confidence: 99%

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Ultrasound‐stimulated peripheral nerve regeneration within asymmetrically porous PLGA/Pluronic F127 nerve guide conduit

Park

Seo

et al. 2010

J Biomed Mater Res

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Recently, we developed a novel method to fabricate a nerve guide conduit (NGC) with asymmetrical pore structure and hydrophilicity using poly(lactic-co-glycolic acid) (PLGA) and Pluronic F127 by a modified immersion precipitation method. From the animal study using a rat model (sciatic nerve defect of rat), we recognized that the unique PLGA/Pluronic F127 tube provided good environments for nerve regeneration. In this study, we applied low-intensity pulsed ultrasound as a simple and noninvasive stimulus at the PLGA/F127 NGC-implanted site transcutaneously in rats to investigate the feasibility of ultrasound for the enhanced nerve regeneration through the tube. The nerve regeneration behaviors within the ultrasound-stimulated PLGA/Pluronic F127 NGCs were compared with the NGCs without the ultrasound treatment as well as normal nerve by histological and immunohistochemical observations. It was observed that the PLGA/Pluronic F127 tube-implanted group applied with the ultrasound had more rapid nerve regeneration behavior (approximately 0.71 mm/day) than the tube-implanted group without the ultrasound treatment (approximately 0.48 mm/day). The ultrasound-treated tube group also showed greater neural tissue area as well as larger axon diameter and thicker myelin sheath than the tube group without the ultrasound treatment, indicating better nerve regeneration. The better nerve regeneration behavior in the our NGC/ultrasound system may be caused by the synergistic effect of the asymmetrically porous PLGA/Pluronic F127 tube with unique properties (selective permeability, hydrophilicity, and structural stability, which can provide good environment for nerve regeneration) and physical stimulus (stimulation of the Schwann cells and activation of the neurotrophic factors).

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Ultrasound‐stimulated peripheral nerve regeneration within asymmetrically porous PLGA/Pluronic F127 nerve guide conduit

Park

Seo

et al. 2010

J Biomed Mater Res

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“…Animals implanted with RA showed the highest mid cross section area within the cohort (p < 0.0001), as expected; whereas all other groups were comparable in this regard (only a significant difference between OxPVA + NGF versus OxPVA, p < 0.05), suggesting a similar regeneration speed. According to the literature, proximal and distal nerve stumps have a growth rate of 0.32 and 0.18 mm/day, respectively, in rat sciatic nerves [ 70 ]. Thus, stumps reconnection across a 5 mm gap may occur in 10 days.…”

Section: Discussionmentioning

confidence: 99%

Development and preclinical evaluation of bioactive nerve conduits for peripheral nerve regeneration: A comparative study

Stocco,

Barbon,

Faccio

et al. 2023

Materials Today Bio

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“…[26] Thus, for the nerve to regenerate across a 15 mm gap, it would have taken at least 30 days (~4 weeks). Hence, nerve conduction velocities at 2 nd and 4 th week postoperatively showed no statistically significant difference.…”

Section: Discussionmentioning

confidence: 99%

Sciatic nerve repair with tissue engineered nerve: Olfactory ensheathing cells seeded poly(lactic-co-glygolic acid) conduit in an animal model

Tan

Htwe

et al. 2013

IJOO

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Background and Aim:Synthetic nerve conduits have been sought for repair of nerve defects as the autologous nerve grafts causes donor site morbidity and possess other drawbacks. Many strategies have been investigated to improve nerve regeneration through synthetic nerve guided conduits. Olfactory ensheathing cells (OECs) that share both Schwann cell and astrocytic characteristics have been shown to promote axonal regeneration after transplantation. The present study was driven by the hypothesis that tissue-engineered poly(lactic-co-glycolic acid) (PLGA) seeded with OECs would improve peripheral nerve regeneration in a long sciatic nerve defect.Materials and Methods:Sciatic nerve gap of 15 mm was created in six adult female Sprague-Dawley rats and implanted with PLGA seeded with OECs. The nerve regeneration was assessed electrophysiologically at 2, 4 and 6 weeks following implantation. Histopathological examination, scanning electron microscopic (SEM) examination and immunohistochemical analysis were performed at the end of the study.Results:Nerve conduction studies revealed a significant improvement of nerve conduction velocities whereby the mean nerve conduction velocity increases from 4.2 ΁ 0.4 m/s at week 2 to 27.3 ΁ 5.7 m/s at week 6 post-implantation (P < 0.0001). Histological analysis revealed presence of spindle-shaped cells. Immunohistochemical analysis further demonstrated the expression of S100 protein in both cell nucleus and the cytoplasm in these cells, hence confirming their Schwann-cell-like property. Under SEM, these cells were found to be actively secreting extracellular matrix.Conclusion:Tissue-engineered PLGA conduit seeded with OECs provided a permissive environment to facilitate nerve regeneration in a small animal model.

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Peripheral nerve repair across a gap studied by repeated observation in a new window implant chamber

Cited by 11 publications

References 22 publications

Ultrasound‐stimulated peripheral nerve regeneration within asymmetrically porous PLGA/Pluronic F127 nerve guide conduit

Ultrasound‐stimulated peripheral nerve regeneration within asymmetrically porous PLGA/Pluronic F127 nerve guide conduit

Development and preclinical evaluation of bioactive nerve conduits for peripheral nerve regeneration: A comparative study

Sciatic nerve repair with tissue engineered nerve: Olfactory ensheathing cells seeded poly(lactic-co-glygolic acid) conduit in an animal model

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