Peripheral nerve regeneration with sustained release of poly(phosphoester) microencapsulated nerve growth factor within nerve guide conduits

Xu, Xiaoyun; Yee, Woon Chee; Hwang, Peter; Yu, Hanry; Wan, Andrew C.A.; Gao, Shujun; Boon, Kum-Loong; Mao, Hai‐Quan; Leong, Kam W.; Wang, Shu

doi:10.1016/s0142-9612(03)00109-1

Cited by 171 publications

(94 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Nerve growth factor (NGF) and glia-derived neurotrophic factor (GDNF), respectively, continuously released by synthetic guidance channels bridging a 15-mm gap in rat sciatic nerves showed different effects on the regeneration of myelinated sensory and motor axons as well as unmyelinated axons (Fine et al, 2002). NGF entrapped in microspheres loaded into synthetic nerve guides bridging a 10-mm gap in rat sciatic nerves showed good regeneration of myelinated fibers also, according to fiber diameter, fiber population, and grade of myelination (Xu et al, 2003). Furthermore, impregnation of fibronectin tubes bridging a 10-mm rat sciatic nerve gap with BDNF, neurotrophin-3 (NT-3), or NT-4 revealed that NT-4 preferentially supports and improves the functional reinnervation of slow motor units, whereas BDNF and NT-3 showed less beneficial effects on regenerating motoneurons (Simon et al, 2003).…”

Section: Role Of Fgf-2 Isoforms Within the Regeneration Scenariomentioning

confidence: 99%

“…Enhanced morphological peripheral nerve regeneration has also been seen after treatment with nerve growth factor (NGF) either slowly released from synthetic nerve guides (Fine et al, 2002) or from NGF-containing polymeric microspheres within synthetic nerve guides (Fine et al, 2002;Xu et al, 2003) or to a even better extent after treatment with glial-derived neurotrophic factor also slowly released from synthetic nerve guides (Fine et al, 2002). Furthermore, it has been shown previously that entrapment of the low molecular weight (18-kDa) isoform of fibroblast growth factor-2 (FGF-2) in synthetic nerve guidance channels is able to enhance growth of myelinated and unmyelinated axons across long gaps significantly (Aebischer et al, 1989).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Differentially promoted peripheral nerve regeneration by grafted Schwann cells over-expressing different FGF-2 isoforms

Haastert

Lipokatic

Fischer

et al. 2006

Neurobiology of Disease

111

118

View full text Add to dashboard Cite

Section: Role Of Fgf-2 Isoforms Within the Regeneration Scenariomentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Differentially promoted peripheral nerve regeneration by grafted Schwann cells over-expressing different FGF-2 isoforms

Haastert

Lipokatic

Fischer

et al. 2006

Neurobiology of Disease

111

118

View full text Add to dashboard Cite

“…3 Tissue bridges may carry regenerating axons from above the lesion to roots or muscles below 3 and could potentially replace the cystic/collagenous areas with functional tissue. Biodegradable polymer implants have been shown to stimulate axon regeneration in the peripheral nervous system by providing structural stability, prolonged release of growth-promoting agents, [4][5][6][7][8][9][10] and a reservoir for sustained therapeutic drug delivery. 11 This approach also provides a platform for evaluating the effectiveness of specific interventions in the spinal cord.…”

Section: Introductionmentioning

confidence: 99%

Sustained Delivery of Dibutyryl Cyclic Adenosine Monophosphate to the Transected Spinal Cord Via Oligo [(Polyethylene Glycol) Fumarate] Hydrogels

Rooney

Knight

Madigan

et al. 2011

Tissue Engineering Part A

View full text Add to dashboard Cite

This study describes the use of oligo [(polyethylene glycol) fumarate] (OPF) hydrogel scaffolds as vehicles for sustained delivery of dibutyryl cyclic adenosine monophosphate (dbcAMP) to the transected spinal cord. dbcAMP was encapsulated in poly(lactic-co-glycolic acid) (PLGA) microspheres, which were embedded within the scaffolds architecture. Functionality of the released dbcAMP was assessed using neurite outgrowth assays in PC12 cells and by delivery to the transected spinal cord within OPF seven channel scaffolds, which had been loaded with Schwann cells or mesenchymal stem cells (MSCs). Our results showed that encapsulation of dbcAMP in microspheres lead to prolonged release and continued functionality in vitro. These microspheres were then successfully incorporated into OPF scaffolds and implanted in the transected thoracic spinal cord. Sustained delivery of dbcAMP inhibited axonal regeneration in the presence of Schwann cells but rescued MSC-induced inhibition of axonal regeneration. dbcAMP was also shown to reduce capillary formation in the presence of MSCs, which was coupled with significant functional improvements. Our findings demonstrate the feasibility of incorporating PLGA microsphere technology for spinal cord transection studies. It represents a novel sustained delivery mechanism within the transected spinal cord and provides a platform for potential delivery of other therapeutic agents.

show abstract

“…The addition of trophic factors, 20,21,72,73 ECM components, 55,86,87 and support cells 33,34,47,74 provides modifications of the tube environment that have been shown to enhance regeneration in the PNS. 89 Although nerve grafts are the standard of care for the management of nerve gaps, the use of AGCs to repair gaps in the PNS offers several potential advantages compared with sural nerve grafts. First, no donor nerve graft needs to be harvested, thus preventing donor site morbidity.…”

Section: Use Of Agcs As Nerve Guides: Tubes To Repair Peripheral Nervmentioning

confidence: 99%

Transplantation of autologous Schwann cells for the repair of segmental peripheral nerve defects

Hood¹,

Levene²,

Levi

2009

FOC

102

View full text Add to dashboard Cite

Peripheral nerve injuries are a source of chronic disability. Incomplete recovery from such injuries results in motor and sensory dysfunction and the potential for the development of chronic pain. The repair of human peripheral nerve injuries with traditional surgical techniques has limited success, particularly when a damaged nerve segment needs to be replaced. An injury to a long segment of peripheral nerve is often repaired using autologous grafting of “noncritical” sensory nerve. Although extensive axonal regeneration can be observed extending into these grafts, recovery of function may be absent or incomplete if the axons fail to reach their intended target. The goal of this review was to summarize the progress that has occurred in developing an artificial neural prosthesis consisting of autologous Schwann cells (SCs), and to detail future directions required in translating this promising therapy to the clinic. In the authors' laboratory, methods are being explored to combine autologous SCs isolated using cell culture techniques with axon guidance channel (AGC) technology to develop the potential to repair critical gap length lesions within the peripheral nervous system. To test the clinical efficacy of such constructs, it is critically important to characterize the fate of the transplanted SCs with regard to cell survival, migration, differentiation, and myelin production. The authors sought to determine whether the use of SC-filled channels is superior or equivalent to strategies that are currently used clinically (for example, autologous nerve grafts). Finally, although many nerve repair paradigms demonstrate evidence of regeneration within the AGC, the authors further sought to determine if the regeneration observed was physiologically relevant by including electrophysiological, behavioral, and pain assessments. If successful, the development of this reparative approach will bring together techniques that are readily available for clinical use and should rapidly accelerate the process of bringing an effective nerve repair strategy to patients with peripheral nerve injury prior to the development of pain and chronic disability.

show abstract

Peripheral nerve regeneration with sustained release of poly(phosphoester) microencapsulated nerve growth factor within nerve guide conduits

Cited by 171 publications

References 49 publications

Differentially promoted peripheral nerve regeneration by grafted Schwann cells over-expressing different FGF-2 isoforms

Differentially promoted peripheral nerve regeneration by grafted Schwann cells over-expressing different FGF-2 isoforms

Sustained Delivery of Dibutyryl Cyclic Adenosine Monophosphate to the Transected Spinal Cord Via Oligo [(Polyethylene Glycol) Fumarate] Hydrogels

Transplantation of autologous Schwann cells for the repair of segmental peripheral nerve defects

Contact Info

Product

Resources

About