Peripheral nerve regeneration by transplantation of bone marrow stromal cell—derived Schwann cells in adult rats

Mimura, Toshiro; Dezawa, Mari; Kanno, Hiroshi; Sawada, Hajime; Yamamoto, Isao

doi:10.3171/jns.2004.101.5.0806

Cited by 176 publications

(128 citation statements)

References 29 publications

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“…Cell-replacement therapies show particular promise in the nervous system, where transplanted embryonic or bone-marrow stem cells have been shown to promote functional recovery in animal models of, for example, spinal cord or peripheral nerve injury (12,15). However, although the therapeutic potential of such transplants is clear, a number of problems remain.…”

Section: Rejoined Sciatic Nerve Contracts the Gastrocnemius Muscle Uponmentioning

confidence: 98%

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Implanted hair follicle stem cells form Schwann cells that support repair of severed peripheral nerves

Amoh

Campillo

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

304

277

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Section: Rejoined Sciatic Nerve Contracts the Gastrocnemius Muscle Uponmentioning

confidence: 98%

“…Some insight into the function of the implanted Schwann cell progenitors is afforded by a previous report (12). After injury to a peripheral nerve, axonal elongation may occur (13).…”

Section: Rejoined Sciatic Nerve Contracts the Gastrocnemius Muscle Uponmentioning

confidence: 99%

Implanted hair follicle stem cells form Schwann cells that support repair of severed peripheral nerves

Amoh

Campillo

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

304

277

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“…After a long distant gap was made in the sciatic nerve in adult rats, MSC-derived Schwann (M-Sch) cells were transplanted into the transected gap and PNS reconstruction evaluated by motor function, electrophysiology and histological analysis up to 6 months after transplantation [43].…”

Section: Pns Regeneration By M-sch Transplantationmentioning

confidence: 99%

Potential of Bone Marrow Stromal Cells in Applications for Neuro-Degenerative, Neuro-Traumatic and Muscle Degenerative Diseases

Dezawa

Ishikawa

Hoshino

et al. 2005

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Cell transplantation is a promising strategy for the treatment of neurodegenerative and muscle degenerative diseases. Many kinds of cells, including embryonic stem cells and tissue stem cells, have been considered as candidates for transplantation therapy. Bone marrow stromal cells (MSCs) have great potential as therapeutic agents since they are easy to isolate and can be expanded from patients without serious ethical or technical problems. We discovered a new method for the highly efficient and specific induction of functional Schwann cells, neurons and skeletal muscle lineage cells from both rat and human MSCs. These induced cells were transplanted into animal models of neurotraumatic injuries, Parkinson's disease, stroke and muscle dystrophies, resulting in the successful integration of transplanted cells and an improvement in behavior of the transplanted animals. Here we focus on the respective potentials of MSCderived cells and discuss the possibility of clinical application in degenerative diseases.

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“…These cells may aid functional and structural aspects of nerve regeneration by secreting a variety of factors that are neurotrophic. Furthermore, they are multipotent and under certain conditions may express Schwann cell markers, or even become Schwann cells (Mimura et al, 2004;Tohill et al, 2004;Zhang et al, 2005;Keilhoff et al, 2006).…”

mentioning

confidence: 99%

Assessment of Axonal Growth into Collagen Nerve Guides Containing VEGF‐Transfected Stem Cells in Matrigel

Kempton

Gonzalez

Leven

et al. 2008

The Anatomical Record

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The early events associated with axonal growth into 10-mm nerve gaps were studied histologically in the rat sciatic nerve model to determine if the outgrowth of blood vessels, Schwann cells, and axons could be enhanced. In the first two experimental groups, collagen nerve guides were filled with either saline or Matrigel. Marrow-derived mesenchymal stem cells (MSCs) were added to Matrigel in two other groups, one of which contained cells transfected with VEGF (MSC/VEGF). After 21 days, the injury site was exposed, fixed, sectioned, and volume fractions of the conduit contents were determined by point counting. The bioresorbable collagen conduits appropriately guided the axons and vessels in a longitudinal direction. The volume fraction of axons was significantly greater in the group with saline when compared with all three groups with Matrigel. This measure had a significant positive correlation with actual counts of myelinated axons. The blood vessel volume fraction in the Matrigel group decreased compared with the saline group, but was restored in the MSC/VEGF group. All Matrigel groups had comparable cellularity and showed a distribution of residual Matrigel in acellular zones. The saline group, by contrast, sustained a network of delicate fibroblastic processes that compartmentalized the nerve and its natural matrix as it became infiltrated by axons as minifascicles. In conclusion, the reduction of axonal outgrowth in the Matrigel groups, when compared with the saline group, suggests that Matrigel may impede the early regenerative process even when enriched by the addition of MSCs or VEGF-transfected cells.

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Peripheral nerve regeneration by transplantation of bone marrow stromal cell—derived Schwann cells in adult rats

Abstract: Results in this study indicate that BMSC-DSCs have great potential to promote regeneration of peripheral nerves. The artificial graft made with BMSC-DSCs represents an alternative method for the difficult reconstruction of a long distance gap in a peripheral nerve.

Cited by 176 publications

References 29 publications

Implanted hair follicle stem cells form Schwann cells that support repair of severed peripheral nerves

Implanted hair follicle stem cells form Schwann cells that support repair of severed peripheral nerves

Potential of Bone Marrow Stromal Cells in Applications for Neuro-Degenerative, Neuro-Traumatic and Muscle Degenerative Diseases

Assessment of Axonal Growth into Collagen Nerve Guides Containing VEGF‐Transfected Stem Cells in Matrigel

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