Peripheral Immunofluorescence of Hepatocytes

Diederichsen, Hans; Riisom, K.; Andersen, Inger Grete Krogh

doi:10.1111/j.1699-0463.1977.tb00441.x

Acta Pathologica Microbiologica Scandinavica Section A Patholog

1977

DOI: 10.1111/j.1699-0463.1977.tb00441.x

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Peripheral Immunofluorescence of Hepatocytes

Hans Diederichsen

K. Riisom

Inger Grete Krogh Andersen

Abstract: By means of indirect immunofluorescence microscopy, a peripheral fluorescence reaction with hepatocytes was found, surrounding the entire cell, with sera from patients with cancer and acute hepatitis and from normal blood donors. I t was proved that this reaction was not related to bile canaEculi, contrary to bile canaliculi antibody demonstrated previously from patients with cronic active liver disease. By using fluorescein-conjugated anti-human IgG, the reaction with the periphery of the hepatocytes was prod… Show more

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Cited by 3 publications

References 15 publications

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Different types of smooth muscle antibodies in chronic active hepatitis and primary biliary cirrhosis: their diagnostic and prognostic significance.

Kurki¹,

Miettinen²,

Linder³

et al. 1980

Gut

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SUMMARY The diagnostic and prognostic significance of the different types of serum smooth muscle antibodies (SMA) were investigated in sera of 24 patients with chronic active hepatitis (CAH) and 15 patients with primary biliary cirrhosis (PBC). SMA of IgG class were found in 92 % of sera from patients with CAH but in only 20 % of sera from PBC patients, whereas the incidence of IgM-SMA was higher in PBC (67 %) than in CAH (38 %). All six patients with the atypical cholestatic form of CAH had SMA of IgM class, whereas other CAH patients had SMA of mainly IgG class. SMA reacting with rabbit liver (bile canaliculus antibodies, BCA) and with rat glomeruli (glomerulus antibodies) were of anti-actin specificity and were more common in CAH than in PBC. Organ specific BCA or glomerulus antibodies were not found. Anti-actin antibodies were detected in the majority of the investigated sera by an immunoenzymatic anti-actin assay. The results suggest that the determination of SMA titres with heavy chain specific antisera may help in the assessment of diagnosis and prognosis of chronic hepatitis.Chronic active hepatitis (CAH) and primary biliary cirrhosis (PBC) are characterised by the presence of high titres of non-organ specific autoantibodies; mitochondrial antibodies (AMA) in PBC and antinuclear antibodies (ANA) and smooth muscle antibodies (SMA) in CAH. These autoantibodies have differential diagnostic importance1 but this is disturbed by the considerable overlap between the serological findings in CAH and PBC. AMA occur in 83-100% of cases with PBC and in 11-35% of cases with CAH. SMA are found in 61-86% of patients with CAH and in 32-49% of patients with PBC.2-8 Antinuclear antibodies are frequently found in both diseases. Some authors have described glomerulus antibodiesg and bile canaliculus antibodies10 11 in CAH and PBC, and a relationship to SMA has been proposed.12-'4The determination of different types of AMA may have diagnostic significance.1' There are also several types of smooth muscle antibodies.'6 17 SMA in

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Different types of smooth muscle antibodies in chronic active hepatitis and primary biliary cirrhosis: their diagnostic and prognostic significance.

Kurki¹,

Miettinen²,

Linder³

et al. 1980

Gut

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show abstract

Anti-actin antibodies revealed by counter-immunoelectrophoresis. Relation to smooth muscle antibodies and bile canalicular antibodies.

Diederichsen¹,

Riisom²

1980

J Clin Pathol

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SUMMARY In investigations by counter-immunoelectrophoresis, anti-actin antibodies were found in 59°of patients with chronic hepatitis and in 8 % of patients with non-hepatic diseases and normal blood donors. Anti-actin antibodies were found more frequently in patients with hepatitis and IgG smooth muscle antibodies than in other groups of diseases and normal subjects with IgG smooth muscle antibodies. Anti-actin antibodies showed no correlation with bile canalicular antibodies.

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Reaction against liver cells by human antiactin antibodies purified by affinity chromatography.

Riison¹,

Diederichsen²,

Andersen³

1982

J Clin Pathol

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SUMMARY Human antiactin antibodies were isolated from human smooth muscle antibodies by affinity chromatography using actin extracted from rabbit striated muscle and coupled to activated CH-Sepharose 4B. The eluate contained IgG and IgM smooth muscle antibodies reacting against muscularis mucosae of the stomach and blood vessels. Further the eluate contained IgM antibodies acting against the surface of isolated hepatocytes and the periphery of hepatocytes of sections of liver cells. These results indicate that antiactin antibodies possibly react with the hepatocyte membrane as has been described with regard to other antibodies.

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Peripheral Immunofluorescence of Hepatocytes

Cited by 3 publications

References 15 publications

Different types of smooth muscle antibodies in chronic active hepatitis and primary biliary cirrhosis: their diagnostic and prognostic significance.

Different types of smooth muscle antibodies in chronic active hepatitis and primary biliary cirrhosis: their diagnostic and prognostic significance.

Anti-actin antibodies revealed by counter-immunoelectrophoresis. Relation to smooth muscle antibodies and bile canalicular antibodies.

Reaction against liver cells by human antiactin antibodies purified by affinity chromatography.

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