Peripheral cells from patients with systemic sclerosis disease co-expressing M1 and M2 monocyte/macrophage surface markers: Relation to the degree of skin involvement

Mohamed, Mai; Gamal, Rania M.; El‐Mokhtar, Mohamed A.; Hassan, Alaa Thabet; Abozaid, Hanan S.; Ghandour, Abeer M.; Ismail, Sahar Abdelmoez A.; Yousef, Hosam A.; El-Hakeim, Eman H.; Makarem, Yasmine S.; Awad, Ahmed Abdellatif

doi:10.1016/j.humimm.2021.03.009

Cited by 13 publications

(7 citation statements)

References 14 publications

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“…The M1 and M2 phenotypes of macrophages were labeled with CD86 and CD206 respectively, and the conversion levels of macrophages in each group were detected by flow cytometry. [ 36 ] The percentages of CD206 − CD86 + (M1) and CD206 + CD86 − (M2) macrophages in the control group are 5.56% and 6.97%, respectively, whereas the percentage of M1 macrophages is significantly increased to 57.34% after stimulation by LPS ( Figure A). After treatment of LPS‐stimulated polarized M1 macrophages with FGMA/FG/PA hydrogel, the percentage of M1 macrophages significantly decreased to 21.60% and the percentage of M2 macrophages increased to 40.35%.…”

Section: Resultsmentioning

confidence: 99%

All‐Natural Immunomodulatory Bioadhesive Hydrogel Promotes Angiogenesis and Diabetic Wound Healing by Regulating Macrophage Heterogeneity

Shi

Wang

et al. 2023

Advanced Science

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Macrophages are highly heterogeneous and exhibit a diversity of functions and phenotypes. They can be divided into pro‐inflammatory macrophages (M1) and anti‐inflammatory macrophages (M2). Diabetic wounds are characterized by a prolonged inflammatory phase and difficulty in healing due to the accumulation of pro‐inflammatory (M1) macrophages in the wound. Therefore, hydrogel dressings with macrophage heterogeneity regulation function hold great promise in promoting diabetic wound healing in clinical applications. However, the precise conversion of pro‐inflammatory M1 to anti‐inflammatory M2 macrophages by simple and biosafe approaches is still a great challenge. Here, an all‐natural hydrogel with the ability to regulate macrophage heterogeneity is developed to promote angiogenesis and diabetic wound healing. The protocatechuic aldehyde hybridized collagen‐based all‐natural hydrogel exhibits good bioadhesive and antibacterial properties as well as reactive oxygen species scavenging ability. More importantly, the hydrogel is able to convert M1 macrophages into M2 macrophages without the need for any additional ingredients or external intervention. This simple and safe immunomodulatory approach shows great application potential for shortening the inflammatory phase of diabetic wound repair and accelerating wound healing.

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Section: Resultsmentioning

confidence: 99%

All‐Natural Immunomodulatory Bioadhesive Hydrogel Promotes Angiogenesis and Diabetic Wound Healing by Regulating Macrophage Heterogeneity

Shi

Wang

et al. 2023

Advanced Science

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“…2 ), which makes us shift our focus to M2 macrophages. A recent study found elevated percentages of monocytes/macrophages in the mixed M1/M2 phenotype in peripheral blood of patients with SSc [ 45 ], which is related with SSc-related interstitial lung disease (SSc-ILD) [ 46 ]; However, Flow assay results from SSc patients and HCs circulating cell showed significantly elevated M1 and M2 macrophage surface markers compared to HCs, no difference when only M1 markers were detected [ 47 ]; Meanwhile, gene-gene interaction networks created based on three transcriptomic datasets from SSc skin biopsies showed that M2 macrophage activation is the core molecular process in the SSc molecular network [ 48 ]. Given the close association of M2 macrophages with SSc fibrosis, we summarize the inhibitors of M2 macrophage polarization that ameliorate SSc and the role of M2 macrophages in SSc.…”

Section: Ssc and M2 Macrophagesmentioning

confidence: 99%

M2 macrophage polarization in systemic sclerosis fibrosis: pathogenic mechanisms and therapeutic effects

Yao

et al. 2023

Heliyon

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“…Traditional views regard M2 as the key role in fibrosis due to it producing profibrotic cytokines including IL-4, IL-13, and transforming growth factor β (TGF-β), leading to overexpression of ECM and scleroderma [ 63 , 66 ]. Indeed, M1 also shows relevance to lung and skin involvement in SSc [ 67 , 68 ]. NLRP3 inflammasome is usually regarded as an inhibitor in M2 activation; however, an increased number of M2 and the activation of NLRP3 inflammasome appear simultaneously in asthma patients [ 69 ].…”

Section: Nlrp3 and Sscmentioning

confidence: 99%

Role of NLRP3 inflammasome in systemic sclerosis

et al. 2022

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Systemic sclerosis (SSc) is an autoimmune rheumatic disease with high mortality, which is featured by inflammation, vascular damage, and aggressive fibrosis. To date, the pathogenesis of SSc remains unclear and effective treatments are still under research. Active NLRP3 recruits downstream proteins such as ASC and caspase-1 and assembles into inflammasome, resulting in excretion of inflammatory cytokines including IL-1β and IL-18, as well as in pyroptosis mediated by gasdermin D. Various studies demonstrated that NLRP3 inflammasome might be involved in the mechanism of tenosynovitis, arthritis, fibrosis, and vascular damage. The pathophysiological changes might be due to the activation of proinflammatory Th2 cells, profibrotic M2 macrophages, B cells, fibroblasts, and endothelial cells. Here, we review the studies focused on NLRP3 inflammasome activation, its association with innate and adaptive immune cells, endothelium injury, and differentiation of fibroblasts in SSc. Furthermore, we summarize the prospect of therapy targeting NLRP3 pathway.

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Peripheral cells from patients with systemic sclerosis disease co-expressing M1 and M2 monocyte/macrophage surface markers: Relation to the degree of skin involvement

Cited by 13 publications

References 14 publications

All‐Natural Immunomodulatory Bioadhesive Hydrogel Promotes Angiogenesis and Diabetic Wound Healing by Regulating Macrophage Heterogeneity

All‐Natural Immunomodulatory Bioadhesive Hydrogel Promotes Angiogenesis and Diabetic Wound Healing by Regulating Macrophage Heterogeneity

M2 macrophage polarization in systemic sclerosis fibrosis: pathogenic mechanisms and therapeutic effects

Role of NLRP3 inflammasome in systemic sclerosis

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