Peripheral blood Th9 cells and eosinophil apoptosis in asthma patients

Hoppenot, Deimantė; Malakauskas, Kęstutis; Lavinskienė, Simona; Bajoriūnienė, Ieva; Kalinauskaitė, Virginija; Sakalauskas, Raimundas

doi:10.1016/j.medici.2015.01.001

Cited by 50 publications

(32 citation statements)

References 28 publications

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“…Consistent with a previous finding that constitutive overexpression of IL‐9 in lung reproduces a part of pathological features of asthma, several murine studies have indicated that Th9 cells play substantial roles in the pathogenesis of asthma . In addition, a recent clinical study has shown that the number of Th9 cells is increased in the peripheral blood in patients with asthma as compared with that in control subjects . These results suggest that Th9 cells also play a role in the pathogenesis of asthma.…”

Section: Roles Of Other Helper T‐cell Subsets In Asthmasupporting

confidence: 86%

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Allergic airway inflammation: key players beyond the Th2 cell pathway

Hirose

Iwata

Tamachi

et al. 2017

Immunological Reviews

110

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Allergic asthma is characterized by eosinophilic airway inflammation, mucus hyperproduction, and airway hyperreactivity, causing reversible airway obstruction. Accumulating evidence indicates that antigen-specific Th2 cells and their cytokines such as IL-4, IL-5, and IL-13 orchestrate these pathognomonic features of asthma. However, over the past decade, the understanding of asthma pathogenesis has made a significant shift from a Th2 cell-dependent, IgE-mediated disease to a more complicated heterogeneous disease. Recent studies clearly show that not only Th2 cytokines but also other T cell-related cytokines such as IL-17A and IL-22 as well as epithelial cell cytokines such as IL-25, IL-33, and thymic stromal lymphopoietin (TSLP) are involved in the pathogenesis of asthma. In this review, we focus on the roles of these players beyond Th2 pathways in the pathogenesis of asthma.

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Section: Roles Of Other Helper T‐cell Subsets In Asthmasupporting

confidence: 86%

“…[83][84][85][86] In addition, a recent clinical study has shown that the number of Th9 cells is increased in the peripheral blood in patients with asthma as compared with that in control subjects. 87 These results suggest that Th9 cells also play a role in the pathogenesis of asthma.…”

Section: Th9 Cells In Allergic Airway Inflammationmentioning

confidence: 89%

Allergic airway inflammation: key players beyond the Th2 cell pathway

Hirose

Iwata

Tamachi

et al. 2017

Immunological Reviews

110

View full text Add to dashboard Cite

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“…In previous study, allergic patients had elevated Th9 cells in peripheral blood mononuclear cells together with elevated serum IgE levels 35. A recent study showed that serum IL-9 concentrations in patients with allergic asthma were increased and inversely related to eosinophil apoptosis 36. Another study reported that IL-9 gene expression in serum samples was increased in asthmatics, which was correlated with disease severity 37.…”

Section: Discussionmentioning

confidence: 96%

Anti-Interleukin-9 Antibody Increases the Effect of Allergen-Specific Immunotherapy in Murine Allergic Rhinitis

Shin

Kim

et al. 2017

Allergy Asthma Immunol Res

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PurposeInterleukin (IL)-9 induces allergic responses; however, the roles of anti-IL-9 antibody in the induction of tolerance remain unclear. This study investigated the effects of anti-IL-9 antibody on oral tolerance (OT) in a mouse model of allergic rhinitis (AR).MethodsBALB/c mice were divided into 4 groups: the control, AR, OT, and OT with anti-IL-9 antibody (OT+IL9AB) groups. Ovalbumin (OVA) was used for sensitization and challenge. Mice in the OT and OT+IL9AB groups were fed OVA for immunotherapy. During immunotherapy, OT+IL9AB mice were injected with anti-IL-9 antibody. Allergic symptoms, tissue eosinophil counts, and serum OVA-specific immunoglobulin E (IgE) were measured. The mRNA expressions of cytokines and transcription factors of T cells of nasal mucosa were determined by real-time polymerase chain reaction (PCR). The protein levels of GATA3, ROR-γt, and Foxp3 in nasal mucosa were determined by Western blot. CD4+CD25+Foxp3+ T cells in the spleen were analyzed by flow cytometry.ResultsAdministration of anti-IL-9 antibody decreased allergic symptoms, OVA-specific IgE levels, and eosinophil counts. In addition, it inhibited T-helper (Th) 2 responses, but had no effect on Th1 responses. Protein levels of ROR-γt and mRNA levels of PU.1 and ROR-γt were reduced by anti-IL-9 antibody. Anti-IL-9 antibody increased Foxp3 and IL-10 mRNA expression, Foxp3 protein, and induction of CD4+CD25+Foxp3+ T cells.ConclusionsAnti-IL-9 antibody decreased allergic inflammation through suppression of Th2 and Th17 cells. Anti-IL-9 antibody enhanced the tolerogenic effects of regulatory T cells. These results suggest that anti-IL-9 antibody might represent a potential therapeutic agent for allergen immunotherapy in patients with uncontrolled allergic airway disease.

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“…The Th22 frequency is currently considered as a biomarker for disease progression in several diseases (89)(90)(91)(92). This finding illustrates that not only Th2 cells are important for allergy, rhinitis and asthma, but also other subsets such as Th9 cells (93), which remain to be further characterized.…”

Section: T-cell-associated Biomarkersmentioning

confidence: 87%

Current and future biomarkers in allergic asthma

Zissler

Bieren

Jakwerth

et al. 2016

Allergy

102

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Diagnosis early in life, sensitization, asthma endotypes, monitoring of disease and treatment progression are key motivations for the exploration of biomarkers for allergic rhinitis and allergic asthma. The number of genes related to allergic rhinitis and allergic asthma increases steadily; however, prognostic genes have not yet entered clinical application. We hypothesize that the combination of multiple genes may generate biomarkers with prognostic potential. The current review attempts to group more than 161 different potential biomarkers involved in respiratory inflammation to pave the way for future classifiers. The potential biomarkers are categorized into either epithelial or infiltrate-derived or mixed origin, epithelial biomarkers. Furthermore, surface markers were grouped into cell-typespecific categories. The current literature provides multiple biomarkers for potential asthma endotypes that are related to T-cell phenotypes such as Th1, Th2, Th9, Th17, Th22 and Tregs and their lead cytokines. Eosinophilic and neutrophilic asthma endotypes are also classified by epithelium-derived CCL-26 and osteopontin, respectively. There are currently about 20 epithelium-derived biomarkers exclusively derived from epithelium, which are likely to innovate biomarker panels as they are easy to sample. This article systematically reviews and categorizes genes and collects current evidence that may promote these biomarkers to become part of allergic rhinitis or allergic asthma classifiers with high prognostic value.

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Peripheral blood Th9 cells and eosinophil apoptosis in asthma patients

Abstract: Patients with allergic asthma demonstrate increased peripheral blood Th9 cells count and serum IL-9, while eosinophil apoptosis is inversely related to IL-9 concentration.

Cited by 50 publications

References 28 publications

Allergic airway inflammation: key players beyond the Th2 cell pathway

Allergic airway inflammation: key players beyond the Th2 cell pathway

Anti-Interleukin-9 Antibody Increases the Effect of Allergen-Specific Immunotherapy in Murine Allergic Rhinitis

Current and future biomarkers in allergic asthma

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