Peripheral Blood Smears

Zabolotzky, Shanon M.; Walker, Dana B.

doi:10.1016/b978-0-323-53314-0.00026-2

Cited by 3 publications

(4 citation statements)

References 57 publications

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“…we did not find the presence of cigar cells in infected mice blood smear, at both the time points (7and 10 p.i) we identified the presence of hypochromic, polychromatic RBCs, spherocytes, stomatocytes and dacrocytes (Fig. S2B) (Ford & Ford, 2013; Zabolotzky & Walker, 1990). Further, our quantitative analysis of these microscopic observations showed ~ 50% drop in the number of normocytic erythrocytes in L. major infected blood as compared to uninfected mice.…”

Section: Resultsmentioning

confidence: 71%

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Localized Leishmania major infection causes systemic iron deficiency that can be controlled by oral iron supplementation

Banerjee

Datta

2022

Preprint

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Leishmania major (L. major) and its related parasitic species infection causes human cutaneous leishmaniasis that results into disfiguring skin lesion. Although L. major infection has been found to alter macrophage iron homeostasis we have limited understanding on whether it can also manipulate the same at systemic level. In fact, localized L. major infection found to promote iron deficiency anemia in children by an unknown mechanism. To address these unresolved issues, Balb/c mouse were infected with L. major and iron status in different organs were monitored systematically with the development of cutaneous lesion. At week 10 post infection when there was maximum lesion development in the parasite infected left hind footpad, the iron content increased significantly in this tissue with the concomitant increase in parasite burden. L. major infection mediated iron accumulation in infected mouse footpad was found to be due to transferrin receptor upregulation and natural resistance-associated macrophage protein 1 (Nramp1) downregulation. Surge in iron level was found to be associated with the reduced hepatic iron storage that resulted increased serum iron. Limited iron storage in liver and bone-marrow of infected mice caused reduced hemoglobin level and production of deformed erythrocytes. Interestingly, L. major infected mice developed splenomegaly with significant upregulation of erythroid related genes. Importantly, oral iron supplementation post infection rescued the development of cutaneous lesion in infected mice. Together, our study unravelled a comprehensive mechanism behind developing iron deficiency anemia during cutaneous leishmaniasis and a novel therapeutic route of treating this infection by delivering iron.

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Section: Resultsmentioning

confidence: 71%

“…Collectively, the presence of these crippled erythrocytes in L. major infected mice indicates development of poikilocytosis at week 7-10 p.i. which is demonstrates a systemic iron deficient condition in these mice (Zabolotzky & Walker, 1990).…”

Section: Resultsmentioning

confidence: 83%

Localized Leishmania major infection causes systemic iron deficiency that can be controlled by oral iron supplementation

Banerjee

Datta

2022

Preprint

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“…3 A ). This led us to critically analyze the morphological appearances of those erythrocytes taking cues from the previously published reports ( 28 , 29 ). At both the time points (weeks 7 and 10 p.i.)…”

Section: Resultsmentioning

confidence: 99%

Localized Leishmania major infection disrupts systemic iron homeostasis that can be controlled by oral iron supplementation

Banerjee¹,

Datta

2023

Journal of Biological Chemistry

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“…It is also noteworthy that lymphocyte counts may remain high in puppies, primarily until the age of 6 months. 35 For this reason, it is essential to consider the age as a factor when evaluating NLR values.…”

Section: Discussionmentioning

confidence: 99%

Neutrophil to lymphocyte ratio as a prognostic biomarker in puppies with acute diarrhea

2021

J Vet Emergen Crit Care

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Peripheral Blood Smears

Cited by 3 publications

References 57 publications

Localized Leishmania major infection causes systemic iron deficiency that can be controlled by oral iron supplementation

Localized Leishmania major infection causes systemic iron deficiency that can be controlled by oral iron supplementation

Localized Leishmania major infection disrupts systemic iron homeostasis that can be controlled by oral iron supplementation

Neutrophil to lymphocyte ratio as a prognostic biomarker in puppies with acute diarrhea

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