Peripheral blood progenitor cell collection by two programs for autologous and allogeneic transplantation

Abstract

Spectra-MNC and Spectra-Auto showed distinct features that should be considered on a case-by-case basis. Similar investigations should be undertaken as new collection platforms are introduced.

Impact of apheresis automation on procedure quality and predictability of CD34+ cell yield

“…A moderately negative influence of WBC precount on CD34 + CE2 has previously been observed on both Spectra Optia and Cobe Spectra . A marked drop in CD34 + CE2 with Cobe Spectra MNC was reported by Cooling et al in patients with WBC precounts exceeding 20 × 10 9 /L while Reinhardt et al observed a modest correlation of the CE2 with WBC precount .…”

“…A moderately negative influence of WBC precount on CD34 1 CE2 has previously been observed on both Spectra Optia 6,8 and Cobe Spectra. 6,8,17 A marked drop in CD34 1 8,18 This slight effect could be explained by the two step separations on the Spectra Optia. The more circulating WBC, the faster the chamber will be filled and the more chambers will be collected.…”

Prospective randomized and crossover comparison of two apheresis machines for peripheral blood stem cell collection: a multicenter study

“…In brief, the freshly obtained samples, which were stained with 7‐AAD and monoclonal antibodies to CD34 and CD45 followed by red blood cell (RBC) lysis, were applied to a single‐platform method. CEs were calculated on the basis of the number of cells that passed through the instrument (CE1) or the TBV of the donors/patients (TBV‐CE), as described previously …”

“…The methods and devices of apheresis for PBSC collection have been improved over the past three decades, and several studies have reported distinct characteristics of apheresis devices and programs . Among these, we previously demonstrated that yields and collection efficiencies (CEs) of CD34+ cells were similar between the automated program of the Spectra (Spectra‐Auto, Software Version 6.1, Terumo BCT) and its manual program (Spectra‐MNC); however, numbers of contaminating platelets (PLTs) were greater in the products collected by the Spectra‐MNC compared with the Spectra‐Auto . Recently, the Spectra Optia (Optia, Terumo BCT) has been introduced as a new instrument with an automated program (Optia MNC) for the apheresis collection of PBSCs .…”

Continuous CD34+ cell collection by a new device is safe and more efficient than by a standard collection procedure: results of a two‐center, crossover, randomized trial

“…Transient thrombocytopenia is an accepted risk of leukapheresis . Reductions of more than 30% from precollection levels are routinely noted.…”