Peripheral blood mononuclear cell microRNA profiles in syphilitic patients with serofast status

Jia, Xiaoyun; Wang, Zhongshuai; Liu, Xiaoke; Zheng, Heyi; Li, Jun

doi:10.1007/s11033-020-05421-7

Cited by 8 publications

(9 citation statements)

References 55 publications

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“… 37 In addition, known and novel predicted (np)-miRNAs in PBMCs in syphilitic patients with SF were identified and verified, such as miR-338-5p, np-miR-163, np-miR-128, np-miR-244, and np-miR-5. 23 It is evident that the exosome-derived miRNAs from serum and cerebrospinal fluid in neurosyphilis and the miRNAs from PBMCs in SF show distinct expression patterns from the exosome-derived miRNAs in plasma from SF. It is challenging to identify common DEmiRNAs across these groups, possibly due to differences in subject selection and individual variation.…”

Section: Discussionmentioning

confidence: 98%

“…19–22 Regarding T. pallidum infection, the miRNA profiles in peripheral blood mononuclear cells (PBMCs) of patients with syphilis were investigated. 23–25 Exosomes were isolated from plasma and cerebrospinal fluid, and DEmiRNAs were associated with neurosyphilis. 26 In our previous studies, 27 we found that exosome-derived miR-146a-5p from T. pallidum -stimulated macrophages targets JAM-C , reducing endothelial cells permeability and monocyte transendothelial migration.…”

Section: Introductionmentioning

confidence: 99%

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Plasma Exosome-Derived microRNAs Profiles in Patients with Serofast Status: A Cross-Sectional Study

Liu¹,

Zhang²,

Lian³

et al. 2023

IJGM

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Purpose Syphilis is a sexually transmitted bacterial infection caused by Treponema pallidum (T. pallidum) , which can lead to chronic morbidity and adverse complications. In clinical practice, serofast status (SF) patients present with clinical symptoms that are very similar to those of healthy individuals or syphilis-cured patients, and often require prolonged follow-up for diagnosis. Currently, there is increasing interest in the potential of plasma exosome-derived miRNA as a biomarker for the detection of infectious diseases. In this study, we aimed to explore the diagnostic potential of miRNA in SF and its possible biological implications. Patients and Methods Exosome-derived miRNAs were isolated from peripheral plasma samples obtained from 20 patients with secondary syphilis (SS), SF, serologically cured syphilis (SC), and healthy controls (HC), and differentially expressed miRNAs (DEmiRNAs) were identified by microarray analysis. Prediction of potential target genes, functional annotation, gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were then performed. The expression of selected miRNAs was confirmed in 37 patients by quantitative reverse transcription polymerase chain reaction (RT-qPCR). A receiver operating characteristic (ROC) analysis was performed to evaluate the diagnostic performance of these miRNAs in differentiating syphilis from HC or SC. Results The expression profile of plasma exosome-derived miRNA was discovered in individuals with SF through microarray analysis. The targeted genes of DEmiRNAs were found to be involved in diverse biological processes according to GO and KEGG analysis, such as regulation of transcription, mitochondria, Golgi, immune system, apoptosis, Ras signaling pathway, etc. Using RT-qPCR validation, miR-1273g-3p, miR-4485-5p, miR-197-3p, and miR-1908-3p showed significant upregulation in patients with SF. These miRNAs exhibited a superior diagnostic ability, either individually or combined, to distinguish SF from SC or HC. Conclusion The DEmiRNAs in plasma exosomes may play a role in the pathogenesis of SF and have the potential to become a noble and effective diagnostic method.

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Plasma Exosome-Derived microRNAs Profiles in Patients with Serofast Status: A Cross-Sectional Study

Liu¹,

Zhang²,

Lian³

et al. 2023

IJGM

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“…(4) Another challenge in the treatment is serofast. The cause of the serofast is still unclear and retreatment to serofast patients can not significantly improve the cure rates ( 71 , 72 ). Prediction of the serofast occurrence is also difficult.…”

Section: Discussionmentioning

confidence: 99%

“…The cause of serofast is still unclear. Possible explanations of the lack of serological response to antisyphilitic therapy include that the patients have undiagnosed neurosyphilis and fail to respond to routine therapy; patients have comorbid HIV, malignancies, or immune diseases; and patients are re-infected by having sex with others ( 71 , 72 ). Prediction of the serofast occurrence is still difficult, and retreatment to serofast patients can not significantly improve the cure rates ( 73 , 74 ).…”

Section: Treatment and Prognosismentioning

confidence: 99%

An Updated Review of Recent Advances in Neurosyphilis

et al. 2022

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Neurosyphilis is caused by Treponema pallidum invading the central nervous system, of which the incidence is increasing worldwide. Due to its variable clinical manifestations, diagnosis of neurosyphilis remains challenging, especially the asymptomatic form. This review focuses on recent advances in neurosyphilis, including epidemiology, clinical manifestations, laboratory findings, comorbidities, diagnosis, treatment, prognosis, and basic research. The expansion of men who have sex with men and the infection of human immunodeficiency virus mainly accounted for the increasing incidence of neurosyphilis. The rate of some historically described forms of neurosyphilis in the pre-antibiotic era declined significantly; atypical features are more prevalent. Neurosyphilis, regarded as a great mimicker for neuro-ophthalmic, audio-vestibular, and psychiatric disorders, often presents concomitantly with other diseases, including metabolic disorders. Studies on long non-coding RNAs, miRNAs, chemokines, and metabolites in peripheral blood and cerebrospinal fluid may facilitate exploring the pathogenesis and identifying novel biomarkers of neurosyphilis. The drug resistance of Treponema pallidum to penicillin has not been reported; ceftriaxone was proposed to be more effective than penicillin, whereas few randomized controlled trials supported this view. This study may pave the way for further research, especially the diagnosis and treatment of neurosyphilis.

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“…Furthermore, miRNA expression differed in peripheral blood mononuclear cells (PBMCs) at various phases of T. pallidum infection [ 32 ]. As a result, the microRNAs in PBMCs might singly or jointly be potential diagnostic biomarkers at different stages of syphilis [ 31 – 35 ]. MiR-195-5p, miR-101-3p, and miR-223-3p alone or in combination can specifically distinguish syphilis patients from non-syphilis patients [ 32 , 33 ]; miR-101-3p can be used as a diagnostic biomarker for patients with primary syphilis [ 31 ].…”

Section: Diagnosismentioning

confidence: 99%

Resurgence of syphilis: focusing on emerging clinical strategies and preclinical models

Xiong,

Liu,

Zhang

et al. 2023

J Transl Med

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Syphilis, a sexually transmitted disease (STD) caused by Treponema pallidum (T. pallidum), has had a worldwide resurgence in recent years and remains a public health threat. As such, there has been a great deal of research into clinical strategies for the disease, including diagnostic biomarkers and possible strategies for treatment and prevention. Although serological testing remains the predominant laboratory diagnostic method for syphilis, it is worth noting that investigations pertaining to the DNA of T. pallidum, non-coding RNAs (ncRNAs), chemokines, and metabolites in peripheral blood, cerebrospinal fluid, and other bodily fluids have the potential to offer novel perspectives on the diagnosis of syphilis. In addition, the global spread of antibiotic resistance, such as macrolides and tetracyclines, has posed significant challenges for the treatment of syphilis. Fortunately, there is still no evidence of penicillin resistance. Hence, penicillin is the recommended course of treatment for syphilis, whereas doxycycline, tetracycline, ceftriaxone, and amoxicillin are viable alternative options. In recent years, efforts to discover a vaccine for syphilis have been reignited with better knowledge of the repertoire of T. pallidum outer membrane proteins (OMPs), which are the most probable syphilis vaccine candidates. However, research on therapeutic interventions and vaccine development for human subjects is limited due to practical and ethical considerations. Thus, the preclinical model is ideal for conducting research, and it plays an important role in clinical transformation. Different preclinical models have recently emerged, such as in vitro culture and mouse models, which will lay a solid foundation for clinical treatment and prevention of syphilis. This review aims to provide a comprehensive summary of the most recent syphilis tactics, including detection, drug resistance treatments, vaccine development, and preclinical models in clinical practice.

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Peripheral blood mononuclear cell microRNA profiles in syphilitic patients with serofast status

Cited by 8 publications

References 55 publications

Plasma Exosome-Derived microRNAs Profiles in Patients with Serofast Status: A Cross-Sectional Study

Plasma Exosome-Derived microRNAs Profiles in Patients with Serofast Status: A Cross-Sectional Study

An Updated Review of Recent Advances in Neurosyphilis

Resurgence of syphilis: focusing on emerging clinical strategies and preclinical models

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