Peripheral Blood Lymphocytes Display Reduced K+ Channel Activity in Aged Humans

Panyi, György; Berecki, Géza; Gáspár, R.; Serés, I.; Fulop, T; Damjanovich, Sándor

doi:10.1006/bbrc.1994.1259

Cited by 8 publications

(4 citation statements)

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“…In this case, the K ϩ current activated at about Ϫ40 mV of membrane potential, with a displacement of 20 mV towards the right of the current-voltage relation with respect to T lymphocytes of the control group. This result is similar to that obtained in T lymphocytes from old men (23).…”

Section: Fig 4 Absence Of Effect Of Moderate Malnutrition On Potasssupporting

confidence: 90%

Effect of Malnutrition on K⁺Current in T Lymphocytes

Fernández

Leehan

Pastrana

et al. 2005

Clin Vaccine Immunol

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Section: Fig 4 Absence Of Effect Of Moderate Malnutrition On Potasssupporting

confidence: 90%

Effect of Malnutrition on K⁺Current in T Lymphocytes

Fernández

Leehan

Pastrana

et al. 2005

Clin Vaccine Immunol

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“…contribute to the observed defects [15][16][17][18]. Several functions of T cells that either partially depend on PKC activity or are regulated by the activation of PKC exhibit abnormalities during aging [19,20].…”

Section: Introductionmentioning

confidence: 99%

Cellular distribution of protein kinase C isozymes in CD3‐mediated stimulation of human T lymphocytes with aging

et al. 1995

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Protein kinase C (PKC) is involved in a variety of cellular responses, such as the expression and secretion of IL-2, the regulation of cytotoxic killing and cell proliferation. It is known that these immune functions are altered with aging. Here, we show that anti-CD3-triggered T cell proliferation is significantly decreased with aging and that H7, an inhibitor of PKC, impairs the anti-CD3-induced T cell proliferation in a differential manner, lymphocytes of healthy young subjects being more sensitive to the PKC inhibitor than those of elderly subjects. We examined (Western blot) the presence and the cellular distribution of PKC isozymes in T lymphocytes of healthy young and elderly subjects in the resting state and after anti-CD3 mAb stimulation using antibodies directed against PKC 6,/3, 8, e and isoforms in the cytosol and the plasma membrane fractions. These five PKC isotypes were present in human T cells of young and elderly subjects. However, their distribution between the cytosolic and membrane fractions varied according to the isozymes and the age of the subjects. In resting lymphocytes of young subjects, all the PKC isozymes were found in the cytosolic fraction, except PKC-~. In resting lymphocytes of elderly subjects PKC-~ and -e were almost equally distributed between the cytosolic and the membrane fractions, whereas PKC-, and -~ were mainly found in the membrane fraction and PKC-/3 was almost exclusively located in the cytosolic fraction. The translocation of PKC-c~, -/3, -~ and -E could be observed under anti-CD3 mAb stimulation in lymphocytes of young subjects, while in the case of elderly subjects only the PKC ]3 isoform was translocated. Our results suggest that the decreased availability of cytosolic PKC may contribute to the diminished PKC-dependent responses to CD3-triggered stimulation of human T lymphocytes with aging.

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“…Sie liegen bei alten Menschen sowohl prozentual als auch absolut erhöht vor (3,16,21), welches mittels der Marker CD16, CD56 bzw. CD57 nachgewiesen wurde.…”

Section: Angeborenes Immunsystemunclassified

“…Selbst das Membranpotential der T-Zellen bleibt von den altersbedingten Veränderun-gen nicht verschont. Nach einer Stimulation kommt es bei T-Zellen alter Menschen zu keiner Verän-derung des Membranpotentials oder aber zu einer Hyperpolarisation, während bei den Jungen eine Depolarisation stattfindet (16). Die Veränderungen im T-Zell-System sind in Tabelle 3 zusammengefasst.…”

Section: N T-zellenunclassified

Das Immunsystem im Alter

Ibs

Rink²

2001

Zeitschrift f�r Gerontologie und Geriatrie

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The immune system changes during life time. In this article we review which kinds of alterations take place. To exclude changes based on illnesses or chronical diseases, the SENIEUR protocol was performed, which defines "healthy elderly people". We focus on alterations in lymphocyte subsets, immunoglobulins and the cytokine network. Monocytes, eosinophils and basophils are without change during life, whereas the number of neutrophils and natural killer (NK) cells increase with aging. T- and B-lymphocytes are diminished in number as well as in function. The serum levels of immunoglobulins are mostly increased. The balance between TH1- and TH2-cells is disturbed and also TH1- and TH2-specific cytokines. Theses changes and their effects on the health status of the elderly are discussed in detail.

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Peripheral Blood Lymphocytes Display Reduced K+ Channel Activity in Aged Humans

Cited by 8 publications

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Effect of Malnutrition on K⁺Current in T Lymphocytes

Effect of Malnutrition on K⁺Current in T Lymphocytes

Cellular distribution of protein kinase C isozymes in CD3‐mediated stimulation of human T lymphocytes with aging

Das Immunsystem im Alter

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Peripheral Blood Lymphocytes Display Reduced K+ Channel Activity in Aged Humans

Cited by 8 publications

References 0 publications

Effect of Malnutrition on K+Current in T Lymphocytes

Effect of Malnutrition on K+Current in T Lymphocytes

Cellular distribution of protein kinase C isozymes in CD3‐mediated stimulation of human T lymphocytes with aging

Das Immunsystem im Alter

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Effect of Malnutrition on K⁺Current in T Lymphocytes

Effect of Malnutrition on K⁺Current in T Lymphocytes