Peripheral Blood Lymphocyte Subsets in Infants with Diarrhea with and without Giardia Lamblia Infection

Schlesinger, Michael; Granot, Esther; Rabinowitz, Ruth; Deckelbaum, Richard J.

doi:10.1203/00006450-199301000-00004

Cited by 8 publications

(7 citation statements)

References 10 publications

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“…However, in-depth investigations are required especially in view of the fact that the parasite remains con¢ned to the intestinal lumen without invading the intestinal mucosa. It may thereby fail to elicit either a systemic in£ammatory response or alterations in lymphocyte subsets in the peripheral blood [55]. The small bowel morphological changes observed during G. lamblia infection are believed to be determined, at least in part, by T cell-dependent activity [13].…”

Section: Resultsmentioning

confidence: 99%

Immune effector responses to an excretory-secretory product of Giardia lamblia

Kaur¹

1999

FEMS Immunology and Medical Microbiology

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The prior immunisation of mice with purified excretory-secretory product (ESP) led to a complete failure of Giardia lamblia colonisation following challenge inoculation of these animals with trophozoites. The prior immunisation of mice with ESP resulted in a significant stimulation of local immunity as evidenced by a significant enhancement of T helper/inducer activity along with a significant increase in immunoglobulin A-bearing cells. Further, the presence of anti-ESP antibodies in the serum of immunised as well as immunised-challenged animals indicated the stimulation of the systemic lymphoid system. This suggests that the ESP is highly immunogenic and it could be one of the major antigens of G. lamblia responsible for protection against the infection.

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Section: Resultsmentioning

confidence: 99%

Immune effector responses to an excretory-secretory product of Giardia lamblia

Kaur¹

1999

FEMS Immunology and Medical Microbiology

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“…Immunophenotyping of lymphocytes in patients with viral infections, immunodeficiencies, and autoimmune diseases has provided clues to the pathogeneses of these diseases (17,23,25,27,31,35,42,51). For diarrheal diseases, very few reports have so far been published (42,45).…”

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confidence: 99%

Shigella infection induces cellular activation of T and B cells and distinct species-related changes in peripheral blood lymphocyte subsets during the course of the disease

et al. 1995

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Immunophenotypic changes in peripheral blood lymphocytes (T, B, and NK cells) in patients during shigellosis was characterized by using triple-color flow cytometry. Eleven Shigella dysenteriae 1-infected adult patients (SDIP), 11 Shigella flexneri-infected adult patients (SFIP), 15 age-and sex-matched healthy controls from Bangladesh (C-B), and 15 healthy volunteers from Sweden (V-S) were studied. In SDIP and SFIP, a significant increase in the CD45RO ؉ cells in both CD4 ؉ and CD8 ؉ T cells was seen. We found evidence for sequential T-cell activation, as shown by increased proportions of CD25 and CD4 ؉ cells; HLA-DR and CD38 on CD8 ؉ cells, and CD54 on CD4 ؉ and CD8 ؉ cells. We found differences in the lymphocyte activation and subset patterns related to the infecting Shigella species. Thus, a decrease in CD45 expression was seen in SFIP; this decrease progressed further during the disease. The proportions of NK cells (CD56 ؉ cells) and CD3 ؊ CD8 ؉ cells out of the total CD8 ؉ cells were increased in SFIP but not in SDIP. The CD3 ؉ CD8 ؉ CD57 ؉ T-cell subset was significantly lower in SDIP than in C-B. The proportion of B-lymphocyte-expressing activation markers CD80 and CD23 was higher in patients than in C-B. There was a significant increase in the proportion of CD4 ؉ T cells and a significant decrease in the percentages of total B cells, the CD3 ؉ CD8 ؉ CD57 ؉ T-cell subset, and the CD56 ؉ CD16 ؉ NK-cell subset for V-S compared with C-B. Our results indicate that distinct subset changes and activation patterns are elicited in SDIP compared with SFIP and also that the degree of activation is related to disease severity. In addition, a common sequence of cell activation was seen during the disease course. The difference in the subset patterns seen in C-B and V-S may be related to differences in the levels or spectra of infectious agents in the environment.

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“…The cells express mainly the CD4 antigen in the lamina propria whereas T cells expressing the CD8 antigen and NK cells comprise the majority of the IEL [56, 57]. It remains to be determined to what degree the local intestinal immune response and changes in intestinal lymphocyte subsets are correlated by those detected in the peripheral blood [55]. Further studies are therefore needed to elucidate the changes in intestinal mucosal lymphocyte subsets occurring during G. lamblia infection and to correlate them with lymphocyte subset distribution in the circulation.…”

Section: Resultsmentioning

confidence: 99%

“…However, in‐depth investigations are required especially in view of the fact that the parasite remains confined to the intestinal lumen without invading the intestinal mucosa. It may thereby fail to elicit either a systemic inflammatory response or alterations in lymphocyte subsets in the peripheral blood [55]. The small bowel morphological changes observed during G. lamblia infection are believed to be determined, at least in part, by T cell‐dependent activity [13].…”

Section: Resultsmentioning

confidence: 99%