Peripheral Blood Leukocytes Interleukin-1 Beta (IL-1β) Cytokine Hyper-Reactivity in Chronic Periodontitis

Sakalauskienė, Jurgina; Giedrimienė, Dalia; Gleiznys, Darius; Gleiznys, Alvydas; Gleiznienė, Rymantė; Vitkauskienė, Astra

doi:10.12659/msm.898422

Cited by 8 publications

(7 citation statements)

References 29 publications

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“…M1 macrophages release the pro-inflammatory cytokines amplifying inflammation in the PAPT milieu ( He et al, 2020 ; Wang L. et al, 2020 ). The higher levels of pro-inflammatory cytokines in PAPT aggravate periodontitis and alveolar bone loss ( Fredriksson et al, 2002 ; Azuma et al, 2014 ; Sakalauskiene et al, 2016 ; Wang D. et al, 2020 ). Chemotactic molecules CCL2, CCL3, and CCL4 play roles in the infiltration of macrophages and other immune cells in PAPT ( Repeke et al, 2010 ; Parisi et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

Downregulation of Macrophage-Specific Act-1 Intensifies Periodontitis and Alveolar Bone Loss Possibly via TNF/NF-κB Signaling

Pathak

Fāng

Chen

et al. 2021

Front. Cell Dev. Biol.

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Periodontitis is a chronic inflammatory oral disease that affects almost half of the adult population. NF-κB activator 1 (Act1) is mainly expressed in immune cells, including macrophages, and modulates immune cells’ function to regulate inflammation in inflammatory diseases. Macrophages play a vital role in the pathophysiology of periodontitis. However, the effect of macrophage-specific Act1 on periodontitis has not been investigated yet. This study aims to unravel the role of macrophage-specific Act1 on the pathophysiology of periodontitis. The expression of Act1 in healthy and periodontitis periodontal tissue was confirmed by immunohistochemistry. Macrophage-specific Act1 expression downregulated (anti-Act1) mice were developed by inserting anti-Act1 antisense oligonucleotides after the CD68 promoter of C57BL/6 mice. Ligature-induced periodontitis (LIP) was induced in anti-Act1 mice and wildtype mice. Micro-CT, histology, and TRAP staining analyzed the periodontal tissue status, alveolar bone loss, and osteoclast numbers. Immunohistochemistry, RT-qPCR, and ELISA analyzed the inflammatory cells infiltration, expression of inflammatory cytokines, and M1/M2 macrophage polarization. mRNA sequencing of in vitro bacterial lipopolysaccharide (LPS)-treated peritoneal macrophages analyzed the differentially expressed genes in anti-Act1 mice during inflammation. Anti-Act1 mice showed aggravated periodontitis and alveolar bone loss compared to wildtype. Periodontitis-affected periodontal tissue (PAPT) of anti-Act1 mice showed a higher degree of macrophage infiltration, and M1 macrophage polarization compared to wildtype. Levels of pro-inflammatory cytokines (IL-1β, IL-6, and TNFα), and macrophage activity-related factors (CCL2, CCL3, and CCL4) were robustly high in PAPT of anti-Act1 mice compared to wildtype. mRNA sequencing and KEGG analysis showed activated TNF/NF-κB signaling in LPS-treated macrophages from anti-Act1 mice. In vitro studies on LPS-treated peritoneal macrophages from anti-act1 mice showed a higher degree of cell migration and expression of inflammatory cytokines, macrophage activity-related factors, M1 macrophage-related factors, and TNF/NF-κB signaling related P-p65 protein. In conclusion, downregulation of macrophage-specific Act1 aggravated periodontitis, alveolar bone loss, macrophage infiltration, inflammation, and M1 macrophage polarization. Furthermore, LPS-treated macrophages from anti-Act1 mice activated TNF/NF-κB signaling. These results indicate the distinct role of macrophage-specific Act1 on the pathophysiology of periodontitis possibly via TNF/NF-κB signaling.

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Section: Discussionmentioning

confidence: 99%

Downregulation of Macrophage-Specific Act-1 Intensifies Periodontitis and Alveolar Bone Loss Possibly via TNF/NF-κB Signaling

Pathak

Fāng

Chen

et al. 2021

Front. Cell Dev. Biol.

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“…In order to determine the severity of inflammation of peri-implant tissues, appropriate indicator can be IL-1β as it is a pro-inflammatory cytokine which regulates the degradation of the connective tissue and modulates the reparative activity indicating the endothelial cells to cause proliferation of fibroblasts and chemotaxis of neutrophils in inflamed gingiva. The levels of IL-1β can help measure the severity of inflammatory periodontal disease [21]. The concentrations of IL-1β in peri-implant crevicular fluid (PICF) are seen to be at a higher level even in the very first stages of peri-implantitis; the inclination for it later to increase is seen related to the progression of the disease [28].…”

Section: Discussionmentioning

confidence: 99%

“…Hank’s balanced salt solution were from Sigma Chemical Co. (St. Louis, MO, USA) and plastic vials were from Carl Rot GmbH & Co. KG (Karlsruhe, Germany) [21].…”

Section: Methodsmentioning

confidence: 99%

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Interleukin-10 and Interleukin-1β Cytokines Expression in Leukocytes of Patients with Chronic Peri-Mucositis

Gleiznys

Abraškevičiūtė

et al. 2019

Med Sci Monit

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BackgroundThe purpose of the present research is to analyze the effect of polyphenols and flavonoids substrat (PFS) from plants Calendula officinalis, Salvia fruticosa, Achillea millefolium, and propolis as immunomodulatory in the production of interleukin (IL)-1β and IL-10 in peripheral blood leukocytes medium (PBLM) in patients who were diagnosed with mucositis of peri-implant tissue compared to patients with healthy implant tissue. It was hypothesized that IL-1β and IL-10 contribute to the inflammation processes noticed in the diseases of peri-implant tissues.Material/MethodsSixty non-smoking patients were included in this study: patients with healthy implants (HP group) and patients with peri-implant mucositis (MP group). Peri-mucositis was diagnosed by radiologic and clinical examination. The PBLM from MP were treated with PFS at various concentrations. The levels of IL-10 and IL-1β excreted by the PBLM stimulated and unstimulated with viable Porphyromonas gingivalis test-tube were committed by the enzyme amplified immunoassay sensitivity method.ResultsUnstimulated and stimulated PBLM and treatment with 5.0 mg/mL or 10.0 mg/mL of PFS in the MP group produced significantly higher levels IL-10 (P<0.001) that analogous mediums of the HP group. The levels of IL-1β decreased more considerably in the stimulated PBLM of the MP group than in those of HP group (P<0.001) after the treatment with PFS at only 10.0 mg/mL concentration.ConclusionsTheses results suggest that the solution of PFS might offer a new potential for the development of a new therapeutic path to prevent and treat peri-implant mucositis.

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“…[1] The problem is that according to many modern studies, there is a positive correlation between inflammatory periodontal disease and many systemic diseases. [3][4][5][6] In the Suzuki et al [7] research it is shown that the infectious periodontitis is associated with hypertrophy of the myocardium due to the activation of metalloproteinase-2. Another group of researchers Li et al [8] is proved that patients with acute myocardial infarct have significantly worse periodontal status.…”

Section: Original Articlementioning

confidence: 99%

Untitled

Tsubanova

2017

AJP

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Introduction: Today in many countries, there is a clear trend to a growing number of major diseases of the oral cavity, due to the poor state of the environment, unbalanced diet, and concomitant chronic diseases of the gastrointestinal tract. Topical problem of modern medicine and pharmacy is optimization of pharmacotherapy of inflammatory periodontal diseases. The aim of the study was to establish optimal combination of new gel for the treatment of periodontal disease. Materials and Methods: The experimental studies were conducted on white male rats. Research of membrane protective activity was carried out on the standard model of spontaneous hemolysis of erythrocytes by Jager. The research of antimicrobial activity was carried out by method of diffusion in agar. Research of Parodont-protective effect was carried out on the model protamine periodontitis. Results and Discussions: Extract of Aloe has powerful membrane protective effect in different doses. The study in vitro showed the high sensitivity of thick oak bark extract in the form of a gel to the cultures of Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis, and Candida аlbicans that allows to recommend it for the treatment of inflammatory diseases of the mouth. Conclusions: According to the results of screening studies, optimal composition of gel that contains Аloe arborescens and cortex Quercus were selected for the treatment of parodontal diseases. Parodont-protective activity of gel is confirmed on the protamine periodontitis model, the ability to decrease the clinical symptoms, namely, hyperemia, edema and necrotic erosive changes of the gums and mucous membranes of the mouth.

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Peripheral Blood Leukocytes Interleukin-1 Beta (IL-1β) Cytokine Hyper-Reactivity in Chronic Periodontitis

Cited by 8 publications

References 29 publications

Downregulation of Macrophage-Specific Act-1 Intensifies Periodontitis and Alveolar Bone Loss Possibly via TNF/NF-κB Signaling

Downregulation of Macrophage-Specific Act-1 Intensifies Periodontitis and Alveolar Bone Loss Possibly via TNF/NF-κB Signaling

Interleukin-10 and Interleukin-1β Cytokines Expression in Leukocytes of Patients with Chronic Peri-Mucositis

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