Peripheral blood involvement in non-Hodgkin's lymphoma detected by clonal gene rearrangement as a biological prognostic marker

Hiorns, Lynne R.; Nicholls, J.; Sloane, J P; Horwich, A.; Ashley, S.; Brada, Michael

doi:10.1038/bjc.1994.63

Cited by 7 publications

(8 citation statements)

References 22 publications

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“…In a study of combined low and high-grade NHL, their detection was shown to be associated with extensive disease, but they were only a marginal independent risk factor for progression-free survival in multivariate analysis 24. The rare cases of DLBCL which actually present in leukaemic phase, however, are associated with chemoresistance and poor outcomes 25…”

Section: Resultsmentioning

confidence: 99%

Bone marrow trephine biopsy involvement by lymphoma: review of histopathological features in 511 specimens and correlation with diagnostic biopsy, aspirate and peripheral blood findings

Sovani¹,

Harvey

Haynes

et al. 2013

J Clin Pathol

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Section: Resultsmentioning

confidence: 99%

Bone marrow trephine biopsy involvement by lymphoma: review of histopathological features in 511 specimens and correlation with diagnostic biopsy, aspirate and peripheral blood findings

Sovani¹,

Harvey

Haynes

et al. 2013

J Clin Pathol

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“…Our data do not support the suggestion that the presence of abnormal circ ulating cells is a manifestation of more e x t e n s i ve disease as has been suggested in human non-Hodgkin's lymphoma. 29 Previous re p o rts of feline lymphos a rcoma have all suggested that atypical (non-lymphoid) tissues are uncommonly affected. 1 , 2 8 Ve ry few cases have been documented, howe ve r, and we failed to find re f e rence to the immunophenotype of a group of such cases.…”

Section: Discussionmentioning

confidence: 99%

Immunophenotypic and histological characterisation of 109 cases of feline lymphosarcoma

1999

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Objective To determine and analyse the immunophenotype and histological appearance of naturally occurring cases of lymphosarcoma in Australian cats. Design A prospective multi‐institutional study of naturally occurring feline lymphosarcoma. Methods One hundred and eighteen cats were referred for diagnosis and/or management of suspected lymphosarcoma. Tissue samples for histopathological analysis and immunophenotyping were collected as biopsies or at necropsy from 109 cases. Histological classification of the neoplasms followed the Working Formulation Classification System. Four multi‐species cross‐reactive antibodies were used to classify tumours as having a B or T cell phenotype. Results Seventy‐six (70%) cases were B cell tumours and 28 (26%) were T cell tumours. The remaining 5 (4%) specimens failed to stain with the four antibodies. Histologically, 11 (10%) cases were classified as low‐grade, 72 (66%) were medium‐grade and 26 (24%) were high‐grade tumours. There were no significant associations between age and either histological grade or immunophenotype. Mediastinal and leukaemic cases were significantly more likely to be T cell tumours (P < 0.001 and P < 0.001, respectively). Conclusions In contrast to previously documented studies in the cat, the majority of cases of lymphosarcoma were of B cell phenotype and intermediate histological grade. Based on our data, the histological phenotype of lymphosarcoma is unlikely to predict immunotype, nor are cases of certain immunotypes likely to be of specific histological subtype. Considered in relation to previous reports, the findings suggest that epidemiological factors operating in these cats to produce lymphosarcoma may be different to those operating in North American and UK cat populations.

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“…Two previous reports have used Southern blotting for stage determination in small (11 high grade) or less well defined series (41 intermediate and high grade) of lymphoma patients. 14,15 Both studies suggested that the molecular detection of circulating lymphoma cells may be of prognostic value. This is the first large study investigating the more sensitive and widely applicable PCR amplification of IgR, which was also controlled by analysis of diagnostic tumor biopsies in the majority of patients.…”

Section: Discussionmentioning

confidence: 99%

“…12,13 The relatively insensitive Southern blot technique has been used to study the involvement of peripheral blood or bone marrow in a variety of NHL subtypes. 14,15 However, there are no large clinical studies evaluating the use of PCR staging in DLBCL.…”

Section: Introductionmentioning

confidence: 99%

Prognostic significance of molecular staging by PCR-amplification of immunoglobulin gene rearrangements in diffuse large B-cell lymphoma (DLBCL)

Mitterbauer-Hohendanner

Mannhalter

Winkler

et al. 2004

Leukemia

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The prognostic value of the detection of peripheral blood (PB) and/or bone marrow (BM) involvement by polymerase chain reaction (PCR) amplification of rearranged immunoglobulin heavy chain (IgH) and immunoglobulin kappa light chain (Igj) genes was evaluated in 155 patients with diffuse large B-cell lymphomas (DLBCL). Immunoglobulin gene rearrangements (IgR) were detected in 35/155 (23%) patients. The presence of IgR in PB/BM was related to clinical stage (CS I-III vs CS IV; Po0.001), histopathological detection of BM involvement (Po0.001), and the International Prognostic Index (Po0.001). IgR-positive cases had a significantly lower complete remission (CR) rate (18/35, 51%) than IgR-negative patients (85/120, 71%; P ¼ 0.042), and a significantly poorer overall survival (OAS) at 5 years (25 vs 66%; Po0.001). There was a significant difference in the estimated OAS at 5 years between patients with negative BM histology and negative PCR results (66%), patients with negative BM histology but positive IgR (37%), and patients with positive BM histology (12%). Our results indicate that molecular methods improve the accuracy of staging in patients with DLBCL and define a group of patients with normal bone marrow histology who have a significantly poorer OAS due to molecular detection of PB/BM involvement.

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Peripheral blood involvement in non-Hodgkin's lymphoma detected by clonal gene rearrangement as a biological prognostic marker

Abstract: SummaryPeripheral blood from 67 patients with non-Hodgkin's lymphoma was examined at initial diagnosis for the presence of circulating lymphoma cells by DNA

Cited by 7 publications

References 22 publications

Bone marrow trephine biopsy involvement by lymphoma: review of histopathological features in 511 specimens and correlation with diagnostic biopsy, aspirate and peripheral blood findings

Bone marrow trephine biopsy involvement by lymphoma: review of histopathological features in 511 specimens and correlation with diagnostic biopsy, aspirate and peripheral blood findings

Immunophenotypic and histological characterisation of 109 cases of feline lymphosarcoma

Prognostic significance of molecular staging by PCR-amplification of immunoglobulin gene rearrangements in diffuse large B-cell lymphoma (DLBCL)

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