Peripheral-blood gene expression profiling studies for coronary artery disease and its severity in Xinjiang population in China

Abstract

BackgroundAlterations in gene expression in peripheral blood cells play a curtail role in the presence and extent of coronary artery disease (CAD), but its severity reflected by gene expression alterations in peripheral blood cells is still unknown in Xinjiang population in China.MethodsGlobal gene expression profiling in peripheral blood was used to explore differentially expressed genes in coronary artery stenosis patients. RNA was extracted from peripheral blood of 9 controls without coronary stenosis and 21 cases with angiographically CAD. The extent of CAD severity was categorized angiographically as no CAD, mild CAD (20 to 50% luminal diameter stenosis [LDS]), moderate CAD (50 to 75% LDS) and severe CAD (≥75% LDS). Differentially expressed genes related with CAD severity from peripheral blood cells were screened by linear mixed effects analysis using the lme4 package in R. Then the differentially expressed genes that gradually up-regulated or down-regulated were enriched by Gene Ontology (GO) functional annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis.ResultsThe most significantly enrichments were toll-like receptor signaling pathway, immune responses, translational processes, cellular growth, inflammation and metabolic processes. Combined with NCBI-GeneRIF and PubMed analysis, we focused on the 12 genes associated with toll-like receptor signaling pathway in the extent of coronary artery stenosis patients. Receiver operating characteristic (ROC) analysis of 12 genes associated with toll-receptor signaling pathway in the 236 CAD patients from GEO database demonstrated that 12 genes expression could predict severe CAD with an area under the curve of 0.67, sensitivity of 77.65% and specificity of 51.52%.ConclusionThese results suggest that 12 genes associated with toll-like receptor signaling pathway in peripheral-blood cells reflect the presence and extent of CAD severity in Xinjiang population in China.Electronic supplementary materialThe online version of this article (10.1186/s12944-018-0798-1) contains supplementary material, which is available to authorized users.

Gene Expression Analysis Suggests Immunological Changes of Peripheral Blood Monocytes in the Progression of Patients With Coronary Artery Disease

“…Previous studies have shown the gene expression of peripheral blood monocytes may be involved in the formation and development of atherosclerosis and CAD. But most studies concentrate on patients in normal and stenosis coronary artery ( Sinnaeve et al, 2009 ; Liu et al, 2018 ), or patients with acute myocardial infarction and stable CAD ( Kiliszek et al, 2012 ; Holvoet et al, 2019 ), which demonstrate little evidence of gradual progression of CAD. In this study, we analyze and compare the gene expression of peripheral blood monocytes in normal coronary artery, intermediate lesion, and myocardial infarction patients with high-throughput sequencing technique.…”

Signature pattern of gene expression and signaling pathway in premature diabetic patients uncover their correlation to early age coronary heart disease

“…It was shown that hyperglycemic condition could activate NF-κβ1, which could trigger several transcriptions of a vast array of genes encoding proinflammatory cytokines such as IL1B, IL6, and IL12Bwas associated in the pathogenesis of atherosclerosis and consequently contributed to modulating the susceptibility to CHD [58][59][60]. Moreover, it was demonstrated that MAPK14 had a proinflammatory gene that was significantly up-regulated in type 2 diabetes mellitus and CHD [61][62][63]. The current study results have shown that the mRNA level of NF-κβ1, IL6, IL12B, and MAPK14 were up-regulated in patients with T2D alone and T2D plus CHD.…”

“…Activated immunity also may be the common antecedent of both T2D and CHD, which probably developed in patients with diabetes and, consequently, coronary atherosclerosis. Furthermore, it was demonstrated in the study by Liu et al that the toll-like receptor signaling pathway interfered in the development of coronary artery stenosis and was associated with CHD severity [62]. Finally, to validate results, a single gene expression analysis of five randomly selected genes was conducted via Q-RT-PCR with increased and different sample sizes.…”

Identification of diagnostic biomarkers and therapeutic targets in peripheral immune landscape from coronary artery disease

“…Previous bioinformatic studies had extensively analyzed a vast amount of microarray data involving CAD from online databases to laboratory experiments. Their results showed that many peripheral immune genes of CAD patients had significant changes compared with the control groups, including but not limited to CXCL8, TNF, SOCS3, TNFAIP3, CD86, C1QB, CD53, C1QC, NCF2, ITGAM, MAPK1, MAPK3, MAPK13, MAPK14, JUN, CHUK, PIK3CB, TLR4, IFNAR1, TLR2, MYD88, IRAK4, CSF3, IL-1A, CCR7 and IL-18 [7][8][9][10]. However, these projects' current sample scale and scope are still limited.…”