Peripheral blood cell separation through surface‐modified polyurethane membranes

Abstract

Cell separation from peripheral blood was investigated using surface-modified polyurethane (PU) membranes with different functional groups. Both red blood cells and platelets could pass through unmodified PU and PU-SO 3 H membranes, whereas the red blood cells preferentially passed through PU-N(C 2 H 5 ) 2 and PU-NHC 2 H 4 OH membranes. The permeation ratio of T and B cells was Ͻ25% for the surface-modified and unmodified PU membranes. CD34 ϩ cells have been recognized as various kinds of stem cells including hematopoietic and mesenchymal stem cells. The adhesiveness of CD34 ϩ cells on the PU membranes was found to be higher than that of red blood cells, platelets, T cells, or B cells. Overall, the adhesiveness of blood cells on the PU membranes increased in the following order: red blood cells Յ platelets Ͻ T cells Յ B cells Ͻ CD34 ϩ cells. Treatment of PU-COOH membranes with a human albumin solution to detach adhered blood cells, allowed recovery of mainly CD34 ϩ cells in the permeate, whereas both red blood cells and platelets could be isolated in the permeate using unmodified PU membranes. The PU membranes showed different permeation and recovery ratios of specific cells depending on the functional groups attached to the membranes.

A new method for the preperative and analytical electrophoresis of cells

“…Such spongy hydrophilic matrices were found suitable as anti-convective supports suitable for the separation of cells with a diameter 5 to 20 μm. Polyurethane is reported to be non-adhesive for RBC and lymphocytes, but adhesive for macrophages [57], an observation confirmed in our experiments. Ag/AgCl electrodes were used in preference to platinum since the latter caused changes in pH when DC current was applied.…”

Separation of hematopoietic stem and progenitor cells from human peripheral blood through polyurethane foaming membranes modified with several amino acids

“…In this study, we prepared not only the PUACOOH membranes used in our previous studies, [7][8][9] but also different types of PUACOOH membranes modified with several amino acids, and conducted further experiments on the separation of CD34 þ cells using these modified membranes. Furthermore, we used a variety of experimental conditions in the permeation and recovery processes, and investigated the effect of permeation volume, type, and concentration of recovery solution on the detachment of CD34 þ cells (i.e., recovery ratio of CD34 þ cells) during the recovery process at 25 C.…”

“…23 Therefore, the transplantation of CD34 þ cells is required in the therapy of patients with acute myeloid leukemia, chronic myeloid leukemia, myelodysplastic syndromes, and systemic mastocytosis. 24 In previous investigations, [7][8][9] cell separation from peripheral blood was investigated using surface-modified polyurethane (PU) membranes with a pore size of 5 lm, carrying different functional groups. CD34 þ cells adhered more strongly to the membranes than red blood cells, platelets, T cells, or B cells.…”

“…[6][7][8][9] Efficient cell separation is an important issue for the successful isolation and purification of blood cells, stem cells, and specific tissue cells. Centrifugation, 10,11 affinity column chromatography, 12,13 fluorescence-activated cell sorting (FACS), 14,15 magnetic cell selection (MACS), 16,17 and membrane filtration [7][8][9][18][19][20] are techniques typically employed for cell separation. Highly purified cellular preparations are obtained using FACS or MACS in conjunction with a fluorescently labeled antibody as the cell-surface marker, whereas the centrifugal separation of cells is commonly used to isolate platelets, leukocytes, mononuclear cells, red blood cells, and non-blood cells.…”

“…CD34 þ cells adhered more strongly to the membranes than red blood cells, platelets, T cells, or B cells. 7 Therefore, purified CD34 þ cells were obtained in the solution recovered from 0.5 wt % human albumin (HSA) solution when this was passed through the membranes after permeation of peripheral blood. We also investigated blood cell permeation through unmodified PU and PU membranes carrying carboxylic acid groups (PUACOOH) with pore sizes of either 5.2 or 12 lm at different blood permeation speeds.…”