Peripheral blood and BM CD34+ CD38− cells show better resistance to cryopreservation than CD34+ CD38+ cells in autologous stem cell transplantation

Ojeda-Uribe, Mario; Sovalat, H.; Bourderont, D; Brunot, Angélique; Marr, Annabell; Lewandowski, H; Chabouté, V; Peter, Pedersen; Hénon, Philippe

doi:10.1080/14653240410011918

Cited by 18 publications

(10 citation statements)

References 40 publications

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“…It is expressed on $1-4% of nucleated cells in BM and on o0.1% of PB. The percentage of late progenitor cells (CD34 þ CD38 þ ) and early progenitor cells (CD34 þ CD38 À ) were found to be significantly different, and our results were consistent with findings reported in the literature [38][39][40].…”

Section: Discussionsupporting

confidence: 95%

Retinal differentiation of human bone marrow-derived stem cells by co-culture with retinal pigment epithelium in vitro

Mathivanan

Trepp

Brunold

et al. 2015

Experimental Cell Research

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Section: Discussionsupporting

confidence: 95%

Retinal differentiation of human bone marrow-derived stem cells by co-culture with retinal pigment epithelium in vitro

Mathivanan

Trepp

Brunold

et al. 2015

Experimental Cell Research

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“…The group was therefore homogeneous with regard to disease, pre-transplant chemotherapy, mobilization chemotherapy and conditioning regimens. In accordance with other reports [20, 22], we observed that the ‘immature’ CD34+ subsets show a better resistance to cryopreservation than ‘mature’ CD34+ subsets, so we considered only the post-thawing count for the correlation with early and long-term engraftment.…”

Section: Discussionsupporting

confidence: 63%

“…Because of the greater fragility of ‘mature’ CD34+ compared to ‘immature’ CD34+ [20,21,22], the subsets of CD34+ cells were evaluated before cryopreservation and after thawing by triple staining with 10 ml fluorescein isothiocyanate-conjugated anti-CD34 (HPCA-2), 10 µl Peridin chlorophyl protein-conjugated anti-CD45 (2D1) and 10 µl phycoerythrin-conjugated anti-CD33, CD38, CD90 or HLA-DR as indicated by Keeney et al [23]. …”

Section: Methodsmentioning

confidence: 99%

Early and Long-Term Engraftment after Autologous Peripheral Stem Cell Transplantation in Acute Myeloid Leukemia Patients

et al. 2006

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This study aimed to identify which subset of CD34+ cells might be the most predictive of early and long-term hematopoietic recovery following autologous peripheral blood stem cell (PBSC) transplantation (PBSCT) in adult acute myeloid leukemia (AML) patients. The relationships between the number of ‘mature’ subsets of CD34+ cells (CD34+/CD33+, CD34+/CD38+, CD34+/DR+ and CD34+/CD90–) and ‘immature’ subsets of CD34+ cells (CD34+/CD33–, CD34+/CD38–, CD34+/DR– and CD34+/CD90+) and early and long-term hemoglobin, neutrophil and platelet counts were studied in a homogeneous series (for disease, pre-transplant chemotherapy, mobilization chemotherapy, conditioning regimen) of 26 AML patients after autologous PBSCT. Cell counts were performed before and after cryopreservation, but only after thawing were the cell counts used for correlation with early and long-term engraftment. The number of CD34+/CD38– cells infused correlated with the neutrophil (r = 0.88, p < 0.005) and platelet counts (r = 0.67, p < 0.05) at 12 months after PBSCT. This correlation was better than that for the total CD34+ cell dose at 12 months (r = 0.36, p = 0.09 for neutrophil count and r = 0.48, p = 0.06 for platelets count). The number of CD34+/CD90+ cells was also correlated with the platelet counts at 6 (r = 0.70, p < 0.05) and 12 months (r = 0.80, p = 0.005) after PBSCT. This correlation was better than the total dose of CD34+ cells at 6 (r = 0.31, p = 0.3) and 12 months (r = 0.48, p = 0.06) for the platelet counts. CD34+ subset analysis suggests that for early engraftment the total number of CD34+ cells infused is more strongly correlated than the CD34+ subsets, whereas the CD34+/CD38– and CD34+/CD90+ subsets may be associated with sustained long-term neutrophil and platelet engraftment. These findings may help to predict the repopulating capacity of PBSCs in AML patients after autologous PBSCT, especially when a relatively low number of CD34+ cells is infused.

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“…Self-renewing HSCs express CD34 for life, whereas selfrenewing HPs express CD34 for only 6-8 weeks (Shizuru et al, 2005;Nielsen and McNagny, 2009). CD34 + and CD38 -cells are considered the best markers of HSCs because these cells have the ability to reconstitute blood constituents (Ojeda-Uribe et al, 2004;Pierre-Louis et al, 2009;Kuranda et al, 2011). However, most CD34…”

Section: Discussionmentioning

confidence: 99%

Vasoactive intestinal polypeptide suppresses proliferation of human cord blood-derived hematopoietic progenitor cells by increasing TNF-α and TGF-β production in the liver

Wang¹,

Xiao²,

Wang³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. The physiology of hepatic hematopoiesis is largely unknown, although studies have indicated that vasoactive intestinal polypeptide (VIP) is involved in this disease. To validate this hypothesis, we assessed the effects of VIP on human cord blood CD34 + cells. We also measured VIP levels and the capacity of vasoactive intestinal polypeptide receptor (VIPR) to bind to VIP in the rat liver during different developmental phases. VIP inhibited the proliferation of cord blood-derived CD34 + cells from concentrations of 10 -7 -10 -12M. The highest suppression was achieved with 10 -8 M VIP at day 10. + cells treated with VIP were increased by 50.70 and 43.46%, respectively. Variations in VIP levels in the rat fetal liver generally increased rapidly with the stage of fetal development. In addition, the affinity of VIPR for VIP increased from relatively low levels in the rat fetal liver and peaked at birth, after which it gradually decreased. VIP had a suppressive effect on the proliferation of human cord blood-derived CD34 + cells, partially by increasing the production of TNF-α and TGF-β. Low VIP levels in the fetal liver and gradually increasing levels after birth may in part be responsible for suppressing hematopoietic stem cell and progenitor proliferation in the liver.

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Peripheral blood and BM CD34+ CD38− cells show better resistance to cryopreservation than CD34+ CD38+ cells in autologous stem cell transplantation

Cited by 18 publications

References 40 publications

Retinal differentiation of human bone marrow-derived stem cells by co-culture with retinal pigment epithelium in vitro

Retinal differentiation of human bone marrow-derived stem cells by co-culture with retinal pigment epithelium in vitro

Early and Long-Term Engraftment after Autologous Peripheral Stem Cell Transplantation in Acute Myeloid Leukemia Patients

Vasoactive intestinal polypeptide suppresses proliferation of human cord blood-derived hematopoietic progenitor cells by increasing TNF-α and TGF-β production in the liver

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