Peripheral benzodiazepine receptor ligands induce apoptosis and cell cycle arrest in human hepatocellular carcinoma cells and enhance chemosensitivity to paclitaxel, docetaxel, doxorubicin and the Bcl-2 inhibitor HA14-1

Sutter, Andreas; Maaser, Kerstin; Grabowski, Patricia; Bradacs, Gesine; Vormbrock, Kirsten; Höpfner, Michael; Krahn, Antje; Heine, Bernhard; Stein, Harald; Somasundaram, Rajan; Schuppan, Detlef; Zeitz, Martin; Scherübl, Hans

doi:10.1016/j.jhep.2004.07.015

Cited by 52 publications

(38 citation statements)

References 39 publications

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“…PK11195 has been reported to generate a reversal action on the chemoresistance in cancer cells, independent of the direct activation of PBR. [25][26][27] In this study, PK11195, a putative chemosensitizer, showed further enhancement of their anticancer effects.…”

Section: Discussionmentioning

confidence: 67%

Comparative Anticancer Effects of Flavonoids and Diazepam in Cultured Cancer Cells

Kim

Lee

et al. 2008

Biological & Pharmaceutical Bulletin

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Section: Discussionmentioning

confidence: 67%

Comparative Anticancer Effects of Flavonoids and Diazepam in Cultured Cancer Cells

Kim

Lee

et al. 2008

Biological & Pharmaceutical Bulletin

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“…Drug-induced changes in cell numbers of EGI-1 and TFK-1 cells were evaluated by crystal violet staining as evaluated by crystal violet staining as previously described [27] . In brief, cells in 96-well microtiter plates were fixed with 10 g/L glutaraldehyde.…”

Section: Measurement Of Growth Inhibitionmentioning

confidence: 99%

“…Preparation of cell lysates and determination of caspase-3 activity were performed as described previously [27] . The activity of caspase-3 was calculated from cleavage of the fluorogenic substrate Ac-DEVD-AMC (Calbiochem, Bad Soden, Germany).…”

Section: Detection Of Apoptosismentioning

confidence: 99%

Histone deacetylase inhibitor MS-275 alone or combined with bortezomib or sorafenib exhibits strong antiproliferative action in human cholangiocarcinoma cells

Baradari

Höpfner²,

Huether³

et al. 2007

WJG

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AIM:To investigate the antiproliferative effect of the histone deacetylase (HDAC) inhibitor MS-275 on cholangiocarcinoma cells alone and in combination with conventional cytostatic drugs (gemcitabine or doxorubicin) or the novel anticancer agents sorafenib or bortezomib. METHODS:Two human bile duct adenocarcinoma cell lines (EGI-1 and TFK-1) were studied. Crystal violet staining was used for detection of cell number changes. Cytotoxicity was determined by measuring the release of the cytoplasmic enzyme lactate dehydrogenase (LDH). Apoptosis was determined by measuring the enzyme activity of caspase-3. Cell cycle status reflected by the DNA content was detected by flow cytometry. RESULTS:MS-275 treatment potently inhibited the proliferation of EGI-1 and TFK-1 cholangiocarcinoma cells by inducing apoptosis and cell cycle arrest. MS-275-induced apoptosis was characterized by activation of caspase-3, up-regulation of Bax and down-regulation of Bcl-2. Cell cycle was predominantly arrested at the G1/S checkpoint, which was associated with induction of the cyclin-dependent kinase inhibitor p21 Waf/CIP1 . Furthermore, additive anti-neoplastic effects were observed when MS-275 treatment was combined with gemcitabine or doxorubicin, while combination with the multikinase inhibitor sorafenib or the proteasome inhibitor bortezomib resulted in overadditive anti-neoplastic effects. CONCLUSION:The growth of human cholangiocarcinoma cells can be potently inhibited by MS-275 alone or in combination with conventional cytostatic drugs or new, targeted anticancer agents.

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“…[10][11][12][13][14][15][16] Furthermore, in some cell types, PK11195 alone is able to induce apoptosis.…”

mentioning

confidence: 99%

The potential anticancer agent PK11195 induces apoptosis irrespective of p53 and ATM status in chronic lymphocytic leukemia cells

Santidrián¹,

Cosialls²,

Coll-Mulet³

et al. 2007

Haematologica

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Peripheral benzodiazepine receptor ligands induce apoptosis and cell cycle arrest in human hepatocellular carcinoma cells and enhance chemosensitivity to paclitaxel, docetaxel, doxorubicin and the Bcl-2 inhibitor HA14-1

Cited by 52 publications

References 39 publications

Comparative Anticancer Effects of Flavonoids and Diazepam in Cultured Cancer Cells

Comparative Anticancer Effects of Flavonoids and Diazepam in Cultured Cancer Cells

Histone deacetylase inhibitor MS-275 alone or combined with bortezomib or sorafenib exhibits strong antiproliferative action in human cholangiocarcinoma cells

The potential anticancer agent PK11195 induces apoptosis irrespective of p53 and ATM status in chronic lymphocytic leukemia cells

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