Periparturient transmission of Cryptosporidium xiaoi from ewes to lambs

Ye, Jianbin; Xiao, Lihua; Wang, Yuanfei; Wang, Lin; Amer, Said; Roellig, Dawn M.; Guo, Yaqiong; Feng, Yaoyu

doi:10.1016/j.vetpar.2013.07.021

Cited by 46 publications

(49 citation statements)

References 48 publications

(53 reference statements)

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“…Molecular studies have identified at least eight Cryptosporidium species in sheep faeces including C. parvum, C. hominis, C. andersoni, C. suis, C. xiaoi, C. fayeri, C. ubiquitum and C. scrofarum, with C. xiaoi, C. ubiquitum and C. parvum most prevalent (Ryan et al, 2005;Santín et al, 2007;Soltane et al, 2007, Geurden et al, 2008, Mueller-Doblies et al, 2008, Quílez et al, 2008a, Fayer and Santín, 2009Giles et al, 2009;Paoletti et al, 2009, Yang et al, 2009Díaz et al, 2010;Robertson et al, 2010;Wang et al, 2010;Fiuza et al, 2011;Shen et al, 2011;Sweeny et al, 2011;Cacciò et al, 2013;Connelly et al, 2013;Imre et al, 2013;Ye et al, 2013). Previous studies conducted in Australia have examined sheep and pre and post-weaned lambs (typically 4 months of age and older) in Western Australia (WA) only (Ryan et al, 2005;Yang et al, 2009;Sweeny et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Longitudinal prevalence, oocyst shedding and molecular characterisation of Cryptosporidium species in sheep across four states in Australia

Yang

Jacobson

Gardner

et al. 2014

Veterinary Parasitology

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This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.Page 1 of 28A c c e p t e d M a n u s c r i p t The prevalence of Cryptosporidium in sheep in the eastern states of Australia has not been 21 well described, therefore a study of the prevalence, oocyst concentration, species and subtypes of 22Cryptosporidium were assessed from lamb faecal samples at three sampling periods (weaning, post-23 weaning and pre-slaughter) from eight farms across South Australia, New South Wales, Victoria 24 and Western Australia. A total of 3,412 faecal samples were collected from approximately 1,182 25 lambs across the 4 states and screened for the presence of Cryptosporidium using a quantitative 26 PCR (qPCR) at the actin locus. Positives were typed at the 18S locus and at a second locus using C. 27 parvum and C. hominis specific qPCR primers. The overall prevalence was 16.9% (95% CI: 15.6-28 18.1%) and of the 576 positives, 500 were successfully genotyped. In general, the prevalence of 29Cryptosporidium was higher in WA than the eastern states. Cryptosporidium prevalence peaked at 30 43.9% and 37.1% at Pingelly (WA2) and Arthur River (WA1) respectively during weaning and at 31

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Section: Introductionmentioning

confidence: 99%

Longitudinal prevalence, oocyst shedding and molecular characterisation of Cryptosporidium species in sheep across four states in Australia

Yang

Jacobson

Gardner

et al. 2014

Veterinary Parasitology

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“…xiaoi was first described in sheep in 2009 (FAYER;SANTIN, 2009). Previously, it was known as C. bovis-like and was found in sheep in the United States (SANTÍN et al, 2007), Spain (NAVARRO-I-MARTINEZ et al, 2007), Tunisia CHALMERS, 2008), United Kingdom (MUELLER-DOBLIES et al, 2008), China (WANG et al, 2010;YE et al, 2013), Norway (ROBERTSON et al, 2010), Australia (SWEENY et al, 2011), andEgypt (MAHFOUZ et al, 2014). In Brazil, the first report of this parasite in sheep was recorded in São Paulo in 2015, where it was found infecting 5-to 360-day-old sheep (ZUCATTO et al, 2015).…”

Section: Resultsmentioning

confidence: 99%

“…Since only one sample from each property was sequenced in this study, it cannot be concluded that this is the only species of Cryptosporidium parasitizing the animals, and other species of the protozoa may be circulating in the environment. There are several species of Cryptosporidium that parasitize sheep, but the most prevalent are C. xiaoi, C. ubiquitum, and C. parvum; the first is commonly found in Australia; the second, in America and Asia; and the third, in Europe YE et al, 2013).…”

Section: Resultsmentioning

confidence: 99%

Occurrence and molecular characterization of Cryptosporidium sp. in sheep

Snak¹,

Smiderle²,

Fernandes³

et al. 2017

SCA

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Considered a zoonosis of utmost importance, cryptosporidiosis has a worldwide distribution and can infect mammals, birds, reptiles, and amphibians. It is caused by a highly resistant protozoan present in the environment and can cause death in immunosuppressed individuals and pups, as well as in farm animals such as cattle and sheep, generating losses. The aim of this study was to investigate the presence of Cryptosporidium spp. in sheep feces from the farms of Western Paraná, which have different management styles, and compare the results with their respective management methods. One hundred and forty-four stool samples were collected (69 from Property 1 and 75 from Property 2) and analyzed using a fecal smear on slides after staining by the modified Ziehl-Neelsen method. Samples tested positive by this method were subjected to nested PCR and the products obtained were sent for sequencing to determine the species. While 82.60% of the samples from Property 1 were tested positive, only 36% of the samples from Property 2 were tested positive. On analyzing the sequencing data, it was observed that the Cryptosporidium species of samples from Property 1 showed high similarity to Cryptosporidium xiaoi and those from Property 2, to Cryptosporidium ubiquitum. The reason for divergence in results can be attributed to differences in management systems adopted by each property, thus showing the importance of detecting carrier animals, as they can contaminate the environment, especially the water sources, and spread the disease to humans and other animals. Key words: Cryptosporidium ubiquitum. Cryptosporidium xiaoi. Nested-PCR. Sheep. Ziehl-Neelsen. ResumoConsiderada uma zoonose de extrema importância, a cryptosporidiose possui distribuição mundial e pode infectar mamíferos, aves, répteis e anfíbios. É causada por um protozoário muito resistente no ambiente, podendo gerar mortes em indivíduos imunossuprimidos e filhotes, principalmente em animais de produção como bovinos e ovinos, gerando prejuízos. O objetivo desse trabalho foi pesquisar a presença de Cryptosporidium spp. em fezes de ovinos de propriedades rurais do Oeste do Paraná, que possuem manejos diferenciados e comparar os resultados encontrados com o respectivo manejo. Foram coletadas 144 amostras de fezes, sendo 69 da Propriedade 1 e 75 da Propriedade 2, analisadas pelo esfregaço de fezes em lâminas após a coloração pelo método de Ziehl-Neelsen modificado. As amostras positivas por esse método foram submetidas à Nested-PCR sendo os produtos obtidos enviados ao sequenciamento para a determinação da espécie. A Propriedade 1 apresentou positividade em 82,60% das amostras, enquanto a Propriedade 2 obteve 36% de amostras positivas. Foi encontrada alta similaridade para Cryptosporidium xiaoi na primeira propriedade e Cryptosporidium ubiquitum na segunda propriedade. A divergência nos resultados pode ser comprovada pela diferente forma de manejo adotada em cada propriedade, mostrando a importância de detectar os animais portadores, uma vez que estes podem contami...

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“…are gastro-intestinal protozoa that affect a wide range of mammals (Geurden et al, 2008;Feng and Xiao, 2011), including sheep. The prevalence of Cryptosporidium and Giardia in sheep varies between studies conducted worldwide but for Cryptosporidium generally ranges from 15%-27% in lambs (Santín et al, 2007;Robertson et al, 2010;Ye et al, 2013;Yang et al, 2014a) and for Giardia generally ranges from 1.5-55.6% (Feng and Xiao, 2011). The genus Cryptosporidium consists of 26 valid species and more than 50 genotypes with C. xiaoi, C. ubiquitum and C. parvum most frequently identified in sheep (Ryan et al, 2005;Santín et al, 2007;Soltane et al, 2007, Geurden et al, 2008, Mueller-Doblies et al, 2008, Quílez et al, 2008, Fayer and Santín, 2009Giles et al, 2009;Paoletti et al, 2009, Yang et al, 2009Díaz et al, 2010;Robertson et al, 2010;Wang et al, 2010;Fiuza et al, 2011;Shen et al, 2011;Sweeny et al, 2011;Sweeny et al, 2012a;Cacciò et al, 2013;Connelly et al, 2013;Imre et al, 2013;Yang et al, 2014a).…”

Section: Introductionmentioning

confidence: 99%

Greater intensity and frequency of Cryptosporidium and Giardia oocyst shedding beyond the neonatal period is associated with reductions in growth, carcase weight and dressing efficiency in sheep

Jacobson

Williams

Yang

et al. 2016

Veterinary Parasitology

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This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Haemonchus status (positive or negative) with lamb production were assessed using general linear models (HSCW and dressing %) and linear mixed effects models (live weight). HighCryptosporidium parvum shedding was associated with lower live weight, ranging 2.31-4.52kg over the 3 sampling occasions. Cryptosporidium parvum shedding was associated 3 with less HSCW in high (3.22kg less) and low (3.22kg less) shedding lambs post-weaning, and high (2.21kg less) and low (2.60kg less) shedding lambs pre-slaughter as well as lower dressing % (2.7% lower in high shedding lambs post-weaning). Cryptosporidium (all species) shedding pre-slaughter was associated with reduced dressing % in both high (1.25% lower) and low (1.21% lower) shedding lambs. Giardia shedding pre-slaughter was associated with 0.59kg less HSCW in high shedding lambs. Increased frequency of C.parvum and Giardia shedding in a specific animal (repeated detection) were associated with Abbreviations:HSCW: hot standard carcase weight WEC: Faecal worm egg count

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Periparturient transmission of Cryptosporidium xiaoi from ewes to lambs

Cited by 46 publications

References 48 publications

Longitudinal prevalence, oocyst shedding and molecular characterisation of Cryptosporidium species in sheep across four states in Australia

Longitudinal prevalence, oocyst shedding and molecular characterisation of Cryptosporidium species in sheep across four states in Australia

Occurrence and molecular characterization of Cryptosporidium sp. in sheep

Greater intensity and frequency of Cryptosporidium and Giardia oocyst shedding beyond the neonatal period is associated with reductions in growth, carcase weight and dressing efficiency in sheep

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