Periostin, secreted from stromal cells, has biphasic effect on cell migration and correlates with the epithelial to mesenchymal transition of human pancreatic cancer cells

Kanno, Atsushi; Satoh, Kennichi; Masamune, Atsushi; Hirota, Morihisa; Kimura, Kenji; Umino, Jun; Hamada, Shin; Satoh, Akihiko; Egawa, Shinichi; Motoi, Fuyuhiko; Unno, Michiaki; Shimosegawa, Tooru

doi:10.1002/ijc.23332

Cited by 121 publications

(113 citation statements)

References 37 publications

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“…However, conclusions vary depending on the type of cancer. POSTN is reported to be capable of enhancing EMT of nicotine-mediated tumor cells in lung and gastric cancer (21,22), whereas exhibits biphasic effects on EMT in pancreatic cancer cells and even has a suppressive role in the regulation of EMT in bladder cancer cells (23,24). The present study aimed to elucidate the reciprocal correlation between POSTN and EMT in ESCC, and revealed that POSTN overexpression was associated with E-cad attenuation as well as Vim enrichment, via immunohistochemistry.…”

Section: Discussionmentioning

confidence: 99%

Association between periostin and epithelial-mesenchymal transition in esophageal squamous cell carcinoma and its clinical significance

Lyu

Wang

et al. 2017

Oncology Letters

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Abstract. The present study aimed to investigate the association between periostin (POSTN), epithelial cadherin (E-cad) and vimentin (Vim) expression levels in esophageal squamous cell carcinoma (ESCC) tissues, and its clinicopathological significance. A total of 58 patients with esophageal cancer were enrolled. Immunohistochemistry was performed to quantify the expression levels of POSTN, E-cad and Vim. E-cad expression was reduced in ESCC tissue, which was associated with severe tumor node metastasis (TNM) stage (P<0.001), lymphatic metastasis (P<0.001) and vascular invasion (P= 0.026). Conversely, Vim expression was found to be increased in ESCC tissues, and had associations with TNM stage (P=0.039) and lymphatic metastasis (P=0.039). POSTN overexpression observed in ESCC cells was associated with attenuation of E-cad expression (P<0.001) and elevated expression levels of Vim (P<0.001). Additionally, significant correlations between the overexpression of POSTN in ESCC cells and clinicopathological variables including TNM staging (P= 0.009), degree of differentiation (P<0.001), lymphatic metastasis (P= 0.009) and vascular invasion (P= 0.002) were verified. Multivariate analysis revealed that overexpression of POSTN in ESCC cancer cells is able to predict the poor prognosis of patients independently of overall survival (P= 0.022) and disease free survival (P=0.019). The preliminary findings of the present study demonstrate that POSTN is involved in the epithelial-mesenchymal transition of ESCC cells, and may therefore be a predictive factor for tumor invasion and metastasis, as well as an indicator of poor prognosis for patients with ESCC.

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Section: Discussionmentioning

confidence: 99%

Association between periostin and epithelial-mesenchymal transition in esophageal squamous cell carcinoma and its clinical significance

Lyu

Wang

et al. 2017

Oncology Letters

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“…It has been suggested recently that the stroma may play an important role in tumor growth and progression and periostin may be a novel candidate protein for regulation of cancer cell behaviour. Periostin has been shown to induce cell migration and also epithelial-mesenchymal transition in pancreatic cancer cells [43], thus it may represent a novel mediator of tumor growth in cholangiocarcinoma. In addition, periostin has been reported to be elevated in serum of some patients with cancers including thymoma and non-small cell lung carcinoma [44].…”

Section: Discussionmentioning

confidence: 99%

Proteomic Analysis of Differentially Expressed Proteins in Peripheral Cholangiocarcinoma

Darby

Vuillier-Devillers

Pinault

et al. 2010

Cancer Microenvironment

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Cholangiocarcinoma is an adenocarcinoma of the liver which has increased in incidence over the last thirty years to reach similar levels to other liver cancers. Diagnosis of this disease is usually late and prognosis is poor, therefore it is of great importance to identify novel candidate markers and potential early indicators of this disease as well as molecules that may be potential therapeutic targets. We have used a proteomic approach to identify differentially expressed proteins in peripheral cholangiocarcinoma cases and compared expression with paired non-tumoral liver tissue from the same patients. Two-dimensional fluorescence difference gel electrophoresis after labeling of the proteins with cyanines 3 and 5 was used to identify differentially expressed proteins. Overall, of the approximately 2,400 protein spots visualised in each gel, 172 protein spots showed significant differences in expression level between tumoral and non-tumoral tissue with p<0.01. Of these, 100 spots corresponding to 138 different proteins were identified by mass spectrometry: 70 proteins were over-expressed whereas 68 proteins were under-expressed in tumoral samples compared to nontumoral samples. Among the over-expressed proteins, immunohistochemistry studies confirmed an increased expression of 14-3-3 protein in tumoral cells while α-smooth muscle actin and periostin were shown to be overexpressed in the stromal myofibroblasts surrounding tumoral cells. α-Smooth muscle actin is a marker of myofibroblast differentiation and has been found to be a Ian A. Darby, Karine Vuillier-Devillers, and Émilie Pinault have equally contributed to this work.

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Section: Discussionmentioning

confidence: 93%

“…As shown for adhesion molecules such as Vcam-1 and Madcam-1, upregulation of the extracellular proteins Periostin and Coch may also ensure a tight association of B cells with FDC during the GC reaction (Fig. 2B) [2,36,37].A more global function of regulating lymphocyte migration within the immune compartments involves sphingosine-1-phosphate (S1P) [38]. However, expression of S1P-generating sphingosin-lipases was not detected in FDC networks (no ''present'' calls) nor in any other compartment of the spleen [39].…”

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confidence: 98%

In silico subtraction approach reveals a close lineage relationship between follicular dendritic cells and BP3^hi stromal cells isolated from SCID mice

et al. 2010

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Organization of the stromal compartments in secondary lymphoid tissue is a prerequisite for an efficient immune reaction. In particular, follicular dendritic cells (FDC) are pivotal for the activation and differentiation of B cells. To investigate the development of FDC, FDC together with tightly associated B cells (FDC networks) were micro-dissected from frozen tissue sections and follicular B cells were sorted by FACS. Using an in silico subtraction approach, gene expression of FDC was determined and compared with that of follicular stromal cells microdissected from the spleen of SCID mice. Nearly 90% of the FDC genes were expressed in follicular stromal cells of the SCID mouse, providing further evidence that FDC develop from the residual network of reticular cells. Thus, it suggests that rather minor modifications in the gene expression profile are sufficient for differentiation into mature FDC. The analysis of different immune-deficient mouse strains shows that a complex pattern of gene regulation controls the development of residual stromal cells into mature FDC. The in silico subtraction approach provides a molecular framework within which to determine the diverse roles of FDC in support of B cells and to investigate the differentiation of FDC from their mesenchymal precursor cells. [1][2][3][4]. The network of FDC is a micro-environment required for the survival of follicular B cells and is also a prerequisite for an efficient GC reaction. At the early stage of GC development FDC support B-cell proliferation, whereas at the later stages FDC have an important function in the selection and differentiation of high affinity B cells to memory and plasma cells [1,5].Although FDC are crucial for B-cell development, our knowledge of FDC transcriptional activity remains marginal. FDC are fragile cells and are tightly associated with B cells -properties that have thus far hampered the isolation of pure FDC populations [6][7]. To overcome these problems, FDC lines have been established, however, as these cells are maintained over several weeks in culture, their phenotype no longer reflects the in vivo situation [8][9][10][11][12][13]. A number of different approaches for the enrichment and gene expression analysis of FDC have been shown to be more representative of the in vivo situation [6,8,11].From a number of experiments, it is apparent that FDC are a highly specialized subset of reticular cells [14][15][16][17][18]. Using BP3, an ectoenzyme of the NAD glycohydrolase family, as a marker antigen for the splenic reticular network of stromal cells, one can distinguish the reticulum cells in the B-cell area from those in the T-cell zone. High expression of BP3 defines the follicle, the area to which B cells To identify molecular markers defining a developmental relationship between mature FDC and the BP3 hi reticular cells of SCID mice, gene expression profiles were determined. Using an in silico subtraction approach, we were able to identify a novel set of genes that showed specific expression in FDC. When gene ex...

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Periostin, secreted from stromal cells, has biphasic effect on cell migration and correlates with the epithelial to mesenchymal transition of human pancreatic cancer cells

Cited by 121 publications

References 37 publications

Association between periostin and epithelial-mesenchymal transition in esophageal squamous cell carcinoma and its clinical significance

Association between periostin and epithelial-mesenchymal transition in esophageal squamous cell carcinoma and its clinical significance

Proteomic Analysis of Differentially Expressed Proteins in Peripheral Cholangiocarcinoma

In silico subtraction approach reveals a close lineage relationship between follicular dendritic cells and BP3^hi stromal cells isolated from SCID mice

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Periostin, secreted from stromal cells, has biphasic effect on cell migration and correlates with the epithelial to mesenchymal transition of human pancreatic cancer cells

Cited by 121 publications

References 37 publications

Association between periostin and epithelial-mesenchymal transition in esophageal squamous cell carcinoma and its clinical significance

Association between periostin and epithelial-mesenchymal transition in esophageal squamous cell carcinoma and its clinical significance

Proteomic Analysis of Differentially Expressed Proteins in Peripheral Cholangiocarcinoma

In silico subtraction approach reveals a close lineage relationship between follicular dendritic cells and BP3hi stromal cells isolated from SCID mice

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In silico subtraction approach reveals a close lineage relationship between follicular dendritic cells and BP3^hi stromal cells isolated from SCID mice