Periostin promotes migration, proliferation, and differentiation of human periodontal ligament mesenchymal stem cells

Abstract

POSTN accelerated the migration, proliferation, and osteogenic differentiation of hPDLSCs.

Periostin promotes migration and osteogenic differentiation of human periodontal ligament mesenchymal stem cells via the Jun amino‐terminal kinases (JNK) pathway under inflammatory conditions

“…37 POSTN, is an ECM protein, promotes cell mobility, adhesion and survival. 5 In present study, we found that the depletion of POSTN reduced the percentage of CD146+ cells and increased the percentage of CD34+ cells in PDLSCs, which suggested revealed that TNFα reduces migration and POSTN expression in PDL cells. 38,39 A former study reported that exogenous POSTN restores TNFα-suppressed migration of PDL cells.…”

“…Passaged cells of primary PDLSCs were cultured in mesenchymal stem cell medium (MSCM, Sciencell, Sandiego, CA, USA) and changed every 3 days. The obtained PDLSCs were characterized as in our previous study . Briefly, cells were immunostained with fluorochrome‐conjugated rabbit anti‐human antibodies against surface markers for 20 minutes at room temperature in the dark.…”

“…35 Briefly, The obtained PDLSCs were characterized as in our previous study. 5 Briefly, cells were immunostained with fluorochrome-conjugated rabbit anti-human antibodies against surface markers for 20 minutes at room temperature in the dark. The stained cells were washed twice with 0.01 M phosphate-buffered saline (PBS) and analysed by a BD FACSCalibur (BD Biosciences, San Jose, CA, USA).…”

“…Periodontal ligament mesenchymal stem cells (PDLSCs) are an optimal choice for the regeneration of PDL due to their ability to generate mineral‐forming cementoblasts, bone‐forming osteoblasts and PDL‐forming fibroblasts in vitro . However, poor adhesion, migration and differentiation of delivered cells limit the therapeutic efficiency when PDLSCs are transplanted in situ to periodontal defects . Previous studies revealed that extracellular matrix (ECM) substrates provide molecular signals, regulate the cell phenotype and are essential for regulating stem cell behaviours …”

Two Transcripts of FBXO5 Promote Migration and Osteogenic Differentiation of Human Periodontal Ligament Mesenchymal Stem Cells

“…hPDLSCs possess osteoblastic and cementoblastic lineages to regenerate periodontal tissue and maintain periodontal ligament integrity [ 4 ]. However, during clinical practice, poor adhesion, migration, and differentiation of delivered cells limit the therapeutic efficiency when hPDLSCs are transplanted in situ to periodontal defects [ 21 ]. Therapeutic intervention should ideally aim at dampening the inflammatory immune response and regenerating the anatomical structure and function of periodontal region [ 22 ].…”