Periostin may play a protective role in the development of eosinophilic chronic rhinosinusitis with nasal polyps in a mouse model

Kim, Sang Wook; Kim, Jin Hyun; Jung, Myeong Hee; Hur, Dong Gu; Lee, Hong Kyoung; Jeon, Sea Yuong; Kim, Dae Woo

doi:10.1002/lary.23786

Cited by 27 publications

(29 citation statements)

References 26 publications

(63 reference statements)

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“…Similarly, many investigators have utilized the NP mouse model using OVA and SEB to show the effects of their candidate drugs or target molecules. Kim et al showed that loss of periostin appeared to enhance polyp‐like lesion formation and mast cell infiltration in a mouse model of eosinophilic rhinosinusitis with NPs . They induced the NP formation in both periostin‐null and wild‐type mice by the repeated nasal administration of OVA and SEB.…”

Section: Discussionmentioning

confidence: 99%

Animal Models in CRS and Pathophysiologic Insights Gained: A Systematic Review

Shin

2016

Laryngoscope Investigative Otolaryngology

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ObjectiveChronic rhinosinusitis (CRS) is a multifactorial inflammatory disease. In particular, CRS with eosinophilic features and/or nasal polyps (NPs) is often recalcitrant to current treatment; thus, appropriate animal models are mandatory to elucidate the pathogenesis of CRS and develop novel and efficient treatment modalities. The author reviewed the recently proposed animal models in CRS and discussed the pathophysiologic insights gained.Data Sources: Articles in the English language referenced in MEDLINE/PubMed from the year 2006 onward (for last 10 years).Review Methods: Review of the literature regarding animal models and related pathologic insights in CRS.ResultsMouse, rabbit, and sheep models of CRS have been used for studying the pathogenesis of CRS. Most of researchers adopted animal models of CRS to prove any molecular mechanisms or therapeutic efficacy. In vitro or human findings and related hypothesis were evaluated in vivo using these models. In addition, novel therapeutic candidates for CRS with or without NP have been applied to animal models.ConclusionAnimal models have elicited insights into the pathogenesis of CRS and also have been useful in testing new treatment modalities. Although there are still clear limitations in the animal studies, newly proposed or revised animal models would be helpful to understand the exact pathophysiology of CRS

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Section: Discussionmentioning

confidence: 99%

Animal Models in CRS and Pathophysiologic Insights Gained: A Systematic Review

Shin

2016

Laryngoscope Investigative Otolaryngology

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“…The primary application of using the allergic model of sinonasal inflammation is testing of novel topical or systemic drugs to reverse inflammation . In order to determine the most sensitive and reliable measure of drug efficacy, we pilot‐tested a common topical anti‐inflammatory drug used in treating CRS, budesonide, in the allergic mouse model.…”

Section: Discussionmentioning

confidence: 99%

“…Determination of tissue eosinophil counts in histologic sections is a common approach to reporting tissue inflammation. Calculation of tissue eosinophils for statistical comparisons, however, carries a high inherent variability . Real‐time polymerase chain reaction (RT‐PCR), on the other hand, is a more quantitative method that may provide a consistent measure of tissue inflammation .…”

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confidence: 99%

Characterization of a novel high‐dose ovalbumin‐induced murine model of allergic sinonasal inflammation

Mendiola¹,

Tharakan²,

Chen³

et al. 2016

Int Forum Allergy Rhinol

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BACKGROUND Few efficacious topical therapies exist for chronic rhinosinusitis. The lack of a reproducible mouse model of CRS limits the pilot testing of potential novel anti-inflammatory therapies. Although the ovalbumin induced mouse model of sinonasal inflammation is commonly used, it is difficult to reproduce and can generate variable histologic results. In this study, we explore a variation of this model in different strains of mice and explore various inflammatory cytokines as reproducible molecular markers of inflammation. METHODS Allergic sinonasal inflammation was generated in BALB/c and C57BL/6 mice using intraperitoneal high dose injections of Ova followed by 10 days of high dose intranasal sensitization. Real-Time PCR for eotaxin, IL4, and IL13 were measured from sinonasal mucosa. We also pilot tested a known topical budesonide to characterize the anti-inflammatory response. Histological sections were analyzed for epithelial thickness and eosinophilia. RESULTS Both BALB/c and C57BL/6 mice consistently showed increases in Th2 cytokines after sensitization with high dose Ova (p<0.0001) when compared to controls. There were also significant increases in epithelial thickening in Ova sensitized mice and eosinophilia in both BALB/c and C57BL/6 strains.In addition, topical budesonide significantly reduced anti-inflammatory cytokines, eosinophilia, and epithelial thickness. CONCLUSION Our variation of the ovalbumin induced mouse model of sinonasal inflammation in both BALB/c and C57BL/6 mice provides an efficacious model for testing potential topical anti-inflammatory therapies for CRS. The utilization of sinonasal mucosal Th2 cytokines along with histologic markers provides a consistent and quantifiable marker of inflammation in assessing the efficacy of candidate drugs.

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“…More recently, Kim et al created a model for recalcitrant allergic CRS by challenging the mice with OVA and Aspergillus protease, which is known to trigger allergic reactions in mouse lungs . However, their original model using OVA and SEB has allowed many great discoveries about the pathophysiology of CRS . In some transgenic strains of mice, small modifications to the concentration, dose, and frequency are required to induce inflammation and nasal polyposis.…”

Section: Introductionmentioning

confidence: 99%

Models for the study of nasal and sinus physiology in health and disease: A review of the literature

Al-Sayed

Agu

Massoud

2017

Laryngoscope Investig Oto

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ObjectiveChronic sinusitis is a very common yet poorly understood medical condition with significant morbidity. Hence, it remains an entity that is difficult to treat with unsatisfactory outcomes of current management options. This necessitates research into the etiology and pathophysiology of the condition to enhance our knowledge and the therapeutic options. Unfortunately, this kind of research is not always feasible on human subjects due to practical and ethical limitations. Therefore, an alternative model that simulates the disease had to be found in order to overcome these limitations. These models could either be in vivo or in vitro. The aim of our review is to summarize the research findings and key discoveries of both in vivo and in vitro models of chronic sinusitis that have enhanced our understanding of the condition today and have paved the way for the future research of tomorrow. Data Sources: PubMed literature review.MethodsA review of the literature was conducted to identify the main successful in vivo and in vitro models for chronic sinusitis.ResultsCreating a successful model for chronic sinusitis is no easy task. Over the years, both in vivo animal models and in vitro tissue culture models were proposed, with each model having its accolades and pitfalls, with the ideal model remaining elusive to this day. However, advancing three‐dimensional cell culturing techniques seems to be a promising new way to find a more accurate model.ConclusionNone of the current models is perfect for a thorough study of chronic sinusitis. However, three‐dimensional cell cultures have the potential to bridge the gap between in vivo and in vitro studies.Level of EvidenceNA

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Periostin may play a protective role in the development of eosinophilic chronic rhinosinusitis with nasal polyps in a mouse model

Abstract: The loss of periostin appears to enhance polyplike lesion formation and mast cell infiltration in a mouse model of eosinophilic rhinosinusitis with nasal polyps.

Cited by 27 publications

References 26 publications

Animal Models in CRS and Pathophysiologic Insights Gained: A Systematic Review

Animal Models in CRS and Pathophysiologic Insights Gained: A Systematic Review

Characterization of a novel high‐dose ovalbumin‐induced murine model of allergic sinonasal inflammation

Models for the study of nasal and sinus physiology in health and disease: A review of the literature

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