Periosteum tissue engineering in an orthotopic in vivo platform

Baldwin, Jeremy; Wagner, Ferdinand; Martine, Laure; Holzapfel, Boris Michael; Theodoropoulos, Christina; Bas, Onur; Savi, Flávia Medeiros; Werner, Carsten; De‐Juan‐Pardo, Elena M.; Hutmacher, Dietmar W.

doi:10.1016/j.biomaterials.2016.11.016

Cited by 82 publications

(59 citation statements)

References 49 publications

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“…In the emerging field of tissue engineering, several alternative approaches have been proposed. Approaches of note include the development of 3D humanized BM structures in vitro 55 56 57 58 59 60 61 62 63 and the orthotopic graft of humanized BM scaffolds in mice 64 . Our approach has the advantage of combining both the complexity of the in vivo system with the easy anatomical accessibility of the humanized tissue graft.…”

Section: Discussionmentioning

confidence: 99%

Bioengineering of Humanized Bone Marrow Microenvironments in Mouse and Their Visualization by Live Imaging

Passaro

Abarrategi

Foster

et al. 2017

JoVE

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Human hematopoietic stem cells (HSCs) reside in the bone marrow (BM) niche, an intricate, multifactorial network of components producing cytokines, growth factors, and extracellular matrix. The ability of HSCs to remain quiescent, self-renew or differentiate, and acquire mutations and become malignant depends upon the complex interactions they establish with different stromal components. To observe the crosstalk between human HSCs and the human BM niche in physiological and pathological conditions, we designed a protocol to ectopically model and image a humanized BM niche in immunodeficient mice. We show that the use of different cellular components allows for the formation of humanized structures and the opportunity to sustain long-term human hematopoietic engraftment. Using two-photon microscopy, we can live-image these structures in situ at the single-cell resolution, providing a powerful new tool for the functional characterization of the human BM microenvironment and its role in regulating normal and malignant hematopoiesis.

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Section: Discussionmentioning

confidence: 99%

Bioengineering of Humanized Bone Marrow Microenvironments in Mouse and Their Visualization by Live Imaging

Passaro

Abarrategi

Foster

et al. 2017

JoVE

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“…In this context, multiphasic scaffolds are defined as having regional morphologies that are distinct from each other . Combined with an approach to closely mimic the natural tissue ultrastructure, multiphasic scaffolds have broad applicability within TE …”

Section: The Effect Of Air Pressure On the Range Of Fiber Diameters Amentioning

confidence: 99%

Dimension‐Based Design of Melt Electrowritten Scaffolds

Hrynevich

Elçi

Haigh

et al. 2018

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The electrohydrodynamic stabilization of direct-written fluid jets is explored to design and manufacture tissue engineering scaffolds based on their desired fiber dimensions. It is demonstrated that melt electrowriting can fabricate a full spectrum of various fibers with discrete diameters (2-50 µm) using a single nozzle. This change in fiber diameter is digitally controlled by combining the mass flow rate to the nozzle with collector speed variations without changing the applied voltage. The greatest spectrum of fiber diameters was achieved by the simultaneous alteration of those parameters during printing. The highest placement accuracy could be achieved when maintaining the collector speed slightly above the critical translation speed. This permits the fabrication of medical-grade poly(ε-caprolactone) into complex multimodal and multiphasic scaffolds, using a single nozzle in a single print. This ability to control fiber diameter during printing opens new design opportunities for accurate scaffold fabrication for biomedical applications.

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“…As such, attempts have been made to engineer constructs that mimic periosteal functions for bone regeneration [ 23 , 24 , 25 , 26 ]. Baldwin et al recently created an orthotopic xenograft model to evaluate their tissue-engineered periosteum, a construct that included osteoprogenitor and vascular compartments [ 27 ]. Their multiphasic construct combined a star-polyethylene glycol (PEG) heparin hydrogel system loaded with human umbilical vein endothelial cells (HUVECs) with a poly (ε-caprolactone) tubular scaffold seeded with human bone marrow-derived MSCs.…”

Section: Dissecting Bone At the Tissue Levelmentioning

confidence: 99%

The Components of Bone and What They Can Teach Us about Regeneration

Nurcombe

Cool

et al. 2017

Materials

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The problem of bone regeneration has engaged both physicians and scientists since the beginning of medicine. Not only can bone heal itself following most injuries, but when it does, the regenerated tissue is often indistinguishable from healthy bone. Problems arise, however, when bone does not heal properly, or when new tissue is needed, such as when two vertebrae are required to fuse to stabilize adjacent spine segments. Despite centuries of research, such procedures still require improved therapeutic methods to be devised. Autologous bone harvesting and grafting is currently still the accepted benchmark, despite drawbacks for clinicians and patients that include limited amounts, donor site morbidity, and variable quality. The necessity for an alternative to this “gold standard” has given rise to a bone-graft and substitute industry, with its central conundrum: what is the best way to regenerate bone? In this review, we dissect bone anatomy to summarize our current understanding of its constituents. We then look at how various components have been employed to improve bone regeneration. Evolving strategies for bone regeneration are then considered.

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Periosteum tissue engineering in an orthotopic in vivo platform

Cited by 82 publications

References 49 publications

Bioengineering of Humanized Bone Marrow Microenvironments in Mouse and Their Visualization by Live Imaging

Bioengineering of Humanized Bone Marrow Microenvironments in Mouse and Their Visualization by Live Imaging

Dimension‐Based Design of Melt Electrowritten Scaffolds

The Components of Bone and What They Can Teach Us about Regeneration

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