Periosteum mechanobiology and mechanistic insights for regenerative
                        medicine

Tate, Melissa L. Knothe; Yu, Na; Jalilian, Iman; Pereira, André F.; Knothe, Ulf

doi:10.1038/bonekey.2016.70

Cited by 16 publications

(15 citation statements)

References 59 publications

(112 reference statements)

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“…A great impetus exists for elucidation of periosteum mechanobiology in the context of regenerative medicine , . A number of in vivo preclinical studies have shown PDCs to be comparable or superior to bone marrow stem cells for bone healing and regeneration , .…”

Section: Discussionmentioning

confidence: 99%

“…The rationale for the choice of the ovine model was twofold. First, we aimed to build on our previous body of work using the ovine model and its translation from in vitro , , to ex vivo to in vivo , , , to clinical studies , , . Also, in contrast to the few cell layer‐thick murine periosteum , the ovine femoral periosteal thickness approximates that of humans , .…”

Section: Methodsmentioning

confidence: 99%

“…Mechanical loading has been shown to correlate with an increase in periosteum's tissue regeneration capacity , . Mechanobiology has been hypothesized to be a key modulator of periosteal stem cell niche quiescence; hence, an understanding of the mechanobiology of the periosteal niche is expected to facilitate use of the tissue and its resident cells in a translational context , .…”

Section: Introductionmentioning

confidence: 99%

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Live Tissue Imaging to Elucidate Mechanical Modulation of Stem Cell Niche Quiescence

O'Brien

Šlapetová

et al. 2016

Stem Cells Translational Medicine

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The periosteum, a composite cellular connective tissue, bounds all nonarticular bone surfaces. Like Velcro, collagenous Sharpey's fibers anchor the periosteum in a prestressed state to the underlying bone. The periosteum provides a niche for mesenchymal stem cells. Periosteal lifting, as well as injury, causes cells residing in the periosteum (PDCs) to change from an immobile, quiescent state to a mobile, active state. The physical cues that activate PDCs to home to and heal injured areas remain a conundrum. An understanding of these cues is key to unlocking periosteum's remarkable regenerative power. We hypothesized that changes in periosteum's baseline stress state modulate the quiescence of its stem cell niche. We report, for the first time, a three‐dimensional, high‐resolution live tissue imaging protocol to observe and characterize ovine PDCs and their niche before and after release of the tissue's endogenous prestress. Loss of prestress results in abrupt shrinkage of the periosteal tissue. At the microscopic scale, loss of prestress results in significantly increased crimping of collagen of periosteum's fibrous layer and a threefold increase in the number of rounded nuclei in the cambium layer. Given the body of published data describing the relationships between stem cell and nucleus shape, structure and function, these observations are consistent with a role for mechanics in the modulation of periosteal niche quiescence. The quantitative characterization of periosteum as a stem cell niche represents a critical step for clinical translation of the periosteum and periosteum substitute‐based implants for tissue defect healing. Stem Cells Translational Medicine 2017;6:285–292

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Live Tissue Imaging to Elucidate Mechanical Modulation of Stem Cell Niche Quiescence

O'Brien

Šlapetová

et al. 2016

Stem Cells Translational Medicine

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“…The robustness of the periosteum is provided by its collagen, while the elasticity of this structure is based on fibers of elastin reinforced by collagenous Sharpey’s fibers anchored to the bone at an angle of 45°. This structural arrangement maintains the periosteal tissue in a state of anisotropic tensional pre-stress ( Knothe Tate et al, 2016 ). Changes in this stress following bone fracture and periosteal lesion leads to deformation of the structural collagen fibrils and cellular nuclei, as well as rapid emigration of cells ( Yu et al, 2017 ).…”

Section: The Periosteal Stem Cell Nichementioning

confidence: 99%

“…Such observations have led to the proposal that the periosteal tissue acts as a mechanosensor and it is assumed that the normal state of stress promotes quiescence in the periosteal stem cell niche ( Knothe Tate et al, 2016 ; Yu et al, 2017 ). Theoretically, this quiescence could be achieved through the action of different proteins such as periostin ( Postn ) on per-SSPCs.…”

Section: The Periosteal Stem Cell Nichementioning

confidence: 99%

Niches for Skeletal Stem Cells of Mesenchymal Origin

Kurenkova

Medvedeva

Newton

et al. 2020

Front. Cell Dev. Biol.

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With very few exceptions, all adult tissues in mammals are maintained and can be renewed by stem cells that self-renew and generate the committed progeny required. These functions are regulated by a specific and in many ways unique microenvironment in stem cell niches. In most cases disruption of an adult stem cell niche leads to depletion of stem cells, followed by impairment of the ability of the tissue in question to maintain its functions. The presence of stem cells, often referred to as mesenchymal stem cells (MSCs) or multipotent bone marrow stromal cells (BMSCs), in the adult skeleton has long been realized. In recent years there has been exceptional progress in identifying and characterizing BMSCs in terms of their capacity to generate specific types of skeletal cells in vivo . Such BMSCs are often referred to as skeletal stem cells (SSCs) or skeletal stem and progenitor cells (SSPCs), with the latter term being used throughout this review. SSPCs have been detected in the bone marrow, periosteum, and growth plate and characterized in vivo on the basis of various genetic markers (i.e., Nestin, Leptin receptor, Gremlin1, Cathepsin-K, etc.). However, the niches in which these cells reside have received less attention. Here, we summarize the current scientific literature on stem cell niches for the SSPCs identified so far and discuss potential factors and environmental cues of importance in these niches in vivo . In this context we focus on (i) articular cartilage, (ii) growth plate cartilage, (iii) periosteum, (iv) the adult endosteal compartment, and (v) the developing endosteal compartment, in that order.

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A Wood‐Derived Periosteum for Spatiotemporal Drug Release: Boosting Bone Repair through Anisotropic Structure and Multiple Functions

Sun,

Chen,

Liu

et al. 2024

Adv Healthcare Materials

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Existing artificial periostea face many challenges, including difficult‐to‐replicate anisotropy in mechanics and structure, poor tissue adhesion, and neglected synergistic angiogenesis and osteogenesis. Here, inspired by natural wood (NW), a wood‐derived elastic artificial periosteum is developed to mimic the structure and functions of natural periosteum, which combines an elastic wood (EW) skeleton, a polydopamine (PDA) binder layer, and layer‐by‐layer (LBL) biofunctional layers. Specifically, EW derived from NW is utilized as the anisotropic skeleton of artificial periosteum to guide cell directional behaviors, moreover, it also shows a similar elastic modulus and flexibility to natural periosteum. To further enhance its synergistic angiogenesis and osteogenesis, surface LBL biofunctional layers are designed to serve as spatiotemporal release platforms to achieve sequential and long‐term release of pamidronate disodium (PDS) and deferoxamine (DFO), which are pre‐encapsulated in chitosan (CS) and hyaluronic acid (HA) solutions, respectively. Furthermore, the combined effect of PDA coating and LBL biofunctional layers enables the periosteum to tightly adhere to damaged bone tissue. More importantly, this novel artificial periosteum can boost angiogenesis and bone formation in vitro and in vivo. This study opens up a new path for biomimetic design of artificial periosteum, and provides a feasible clinical strategy for bone repair.

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Periosteum mechanobiology and mechanistic insights for regenerative medicine

Cited by 16 publications

References 59 publications

Live Tissue Imaging to Elucidate Mechanical Modulation of Stem Cell Niche Quiescence

Live Tissue Imaging to Elucidate Mechanical Modulation of Stem Cell Niche Quiescence

Niches for Skeletal Stem Cells of Mesenchymal Origin

A Wood‐Derived Periosteum for Spatiotemporal Drug Release: Boosting Bone Repair through Anisotropic Structure and Multiple Functions

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