Periodontitis promotes the expression of gingival transmembrane serine protease 2 (TMPRSS2), a priming protease for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)

Ohnishi, Tomokazu; Nakamura, Toshiaki; Shima, Kaori; Noguchi, Kazuyuki; Chiba, Norika; Matsuguchi, Tetsuya

doi:10.1016/j.job.2022.04.004

Cited by 8 publications

(5 citation statements)

References 41 publications

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“…In the spinous cell layer of epithelia, not basal layer. Sakaguchi et al, 2020;Drozdzik and Drozdzik, 2022;Ohnishi et al, 2022 Buccal mucosa Human In the non-keratinized stratified squamous epithelia. Suprabasal >basal cells.…”

Section: Dysfunction Of the Nervous Systemmentioning

confidence: 99%

The oral manifestations and related mechanisms of COVID-19 caused by SARS-CoV-2 infection

Lin

Gao

Wang

et al. 2023

Front. Cell. Neurosci.

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Coronavirus disease 2019 (COVID-19) was reported to be associated with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection, and patients present mostly with respiratory symptoms. There have been an increasing number of reports on oral manifestations, and some of these signs are informative in terms of identifying SARS-CoV-2 infection. The goal of present study was to review and synthesize the clinical characteristics and underlying mechanisms of COVID-19 oral manifestations, as well as to evaluate the factors influencing SARS-CoV-2 infectivity, in order to conduct further in-depth investigations and help clinicians diagnose COVID-19 patients exhibiting oral symptoms.

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Section: Dysfunction Of the Nervous Systemmentioning

confidence: 99%

The oral manifestations and related mechanisms of COVID-19 caused by SARS-CoV-2 infection

Lin

Gao

Wang

et al. 2023

Front. Cell. Neurosci.

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show abstract

“…Additionally, periodontal inflammation promotes the inflammatory and innate immune responses by facilitating the production of reactive oxygen species, which damages DNA in cells [ 27 ]. Moreover, induced DNA damage can cause lung epithelial cell malfunction, which favors SARS-CoV-2 invasion [ 28 ]. Even though several pertinent studies on the connection between COVID-19 and periodontitis have been done, more research are needed to be done on the probable mechanisms and biomarkers that connect the two diseases.…”

Section: Discussionmentioning

confidence: 99%

Potential links between COVID-19 and periodontitis: a bioinformatic analysis based on GEO datasets

Zhang

Sun²,

Xu³

et al. 2022

BMC Oral Health

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Background 2019 Coronavirus disease (COVID-19) is an infectious disease caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The COVID-19 pandemic has already had a serious influence on human existence, causing a huge public health concern for countries all around the world. Because SARS-CoV-2 infection can be spread by contact with the oral cavity, the link between oral illness and COVID-19 is gaining traction. Through bioinformatics approaches, we explored the possible molecular mechanisms linking the COVID-19 and periodontitis to provide the basis and direction for future research. Methods Transcriptomic data from blood samples of patients with COVID-19 and periodontitis was downloaded from the Gene Expression Omnibus database. The shared differentially expressed genes were identified. The analysis of Gene Ontology, Kyoto Encyclopedia of Genesand Genomes pathway, and protein–protein interaction network was conducted for the shared differentially expressed genes. Top 5 hub genes were selected through Maximal Clique Centrality algorithm. Then mRNA-miRNA network of the hub genes was established based on miRDB database, miRTarbase database and Targetscan database. The Least absolute shrinkage and selection operator regression analysis was used to discover possible biomarkers, which were then investigated in relation to immune-related genes. Results Fifty-six shared genes were identified through differential expression analysis in COVID-19 and periodontitis. The function of these genes was enriched in regulation of hormone secretion, regulation of secretion by cell. Myozenin 2 was identified through Least absolute shrinkage and selection operator regression Analysis, which was down-regulated in both COVID-19 and periodontitis. There was a positive correlation between Myozenin 2 and the biomarker of activated B cell, memory B cell, effector memory CD4 T cell, Type 17 helper cell, T follicular helper cell and Type 2 helper cell. Conclusion By bioinformatics analysis, Myozenin 2 is predicted to correlate to the pathogenesis and immune infiltrating of COVID-19 and periodontitis. However, more clinical and experimental researches are needed to validate the function of Myozenin 2.

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“…However, only the spike protein, which is activated by proteolysis, can release the fusion peptide into the target cell in a controlled manner [ 27 ], resulting in cell infection. The main hydrolase implicated in the viral infection process is TMPRSS2 [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

Kinetics of Drug Molecule Interactions with a Newly Developed Nano-Gold-Modified Spike Protein Electrochemical Receptor Sensor

Liu

Wang

et al. 2022

Biosensors

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In March 2020, the World Health Organization (WHO) declared COVID-19 a pandemic, and the spike protein has been reported to be an important drug target for anti-COVID-19 treatment. As such, in this study, we successfully developed a novel electrochemical receptor biosensor by immobilizing the SARS-CoV-2 spike protein and using AuNPs-HRP as an electrochemical signal amplification system. Moreover, the time-current method was used to quantify seven antiviral drug compounds, such as arbidol and chloroquine diphosphate. The results show that the spike protein and the drugs are linearly correlated within a certain concentration range and that the detection sensitivity of the sensor is extremely high. In the low concentration range of linear response, the kinetics of receptor–ligand interactions are similar to that of an enzymatic reaction. Among the investigated drug molecules, bromhexine exhibits the smallest Ka value, and thus, is most sensitively detected by the sensor. Hydroxychloroquine exhibits the largest Ka value. Molecular docking simulations of the spike protein with six small-molecule drugs show that residues of this protein, such as Asp, Trp, Asn, and Gln, form hydrogen bonds with the -OH or -NH2 groups on the branched chains of small-molecule drugs. The electrochemical receptor biosensor can directly quantify the interaction between the spike protein and drugs such as abidor and hydroxychloroquine and perform kinetic studies with a limit of detection 3.3 × 10−20 mol/L, which provides a new research method and idea for receptor–ligand interactions and pharmacodynamic evaluation.

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Periodontitis promotes the expression of gingival transmembrane serine protease 2 (TMPRSS2), a priming protease for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)

Cited by 8 publications

References 41 publications

The oral manifestations and related mechanisms of COVID-19 caused by SARS-CoV-2 infection

The oral manifestations and related mechanisms of COVID-19 caused by SARS-CoV-2 infection

Potential links between COVID-19 and periodontitis: a bioinformatic analysis based on GEO datasets

Kinetics of Drug Molecule Interactions with a Newly Developed Nano-Gold-Modified Spike Protein Electrochemical Receptor Sensor

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