Periodontal Regenerative Treatment of Intrabony Defects Associated with Palatal Grooves: A Report of Two Cases

Corbella, Stefano; Alberti, Alice; Zotti, Beatrice; Francetti, Luca

doi:10.1155/2019/8093192

Cited by 8 publications

(4 citation statements)

References 34 publications

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“…Additionally, it was hypothesized that using an enamel matrix derivative could help patients whose inflammatory processes were fast progressing and causing the degeneration of periodontal tissue [ 29 ]. Additionally, severe intrabony flaws linked with the palatal radicular groove were successfully treated without endodontic therapy or retreatment by applying an enamel matrix derivative [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of Enamel Matrix Derivative on Cell Spheroids Made of Stem Cells Obtained from the Gingiva on Osteogenic Differentiation

Hwa

Lee

et al. 2023

Medicina

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Background and Objectives: A derivative of the enamel matrix was used to speed up periodontal regeneration, including the formation of new cementum, alveolar bone, and periodontal ligament. In this study, human gingiva-derived stem cell–derived cell spheroids were used to assess the effects of an enamel matrix derivative on cell viability, osteogenic differentiation, and mineralization. Materials and Methods: Human gingiva-derived stem cells were used to create spheroids, which were then coupled with unloaded control groups and an enamel matrix derivative at a final concentration of 2.7, 27, 270, and 2700 μg/mL. The morphological examination of the created stem cell spheroids took place on days 1, 3, 5, and 7. The Live/Dead Kit assay was used to determine the qualitative viability of cells on days 3 and 7. Using the Cell Counting Kit-8, the quantitative vitality of the cell spheroids was assessed on days 1, 3, and 5. On days 7 and 14, alkaline phosphatase activity assays and Alizarin Red S staining were carried out to examine the osteogenic differentiation of the cell spheroids. RUNX2 and COL1A1 expression levels on days 7 and 14 were determined using real-time polymerase chain reaction. Results: The added enamel matrix derivative at the tested concentrations did not significantly alter the morphology of the applied stem cells’ well-formed spheroids on day 1. On days 3 and 7, the majority of the spheroids’ cells fluoresced green while they were being cultivated. Alkaline phosphatase activity data revealed a substantial rise in the 2700 μg/mL group on day 7 when compared to the unloaded control (p < 0.05). On days 7 and 14, calcium deposits were distinctly seen in each group. In the 27 and 2700 μg/mL groups, the treatment with the enamel matrix derivative resulted in noticeably higher values for the Alizarin Red S staining (p < 0.05). qPCR results showed that adding an enamel matrix derivative to the culture of the 27 μg/mL group raised the level of RUNX2 mRNA expression. Conclusions: These results lead us to the conclusion that a derivative of the enamel matrix may be used to promote osteogenic differentiation in stem cell spheroids.

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Section: Discussionmentioning

confidence: 99%

Effects of Enamel Matrix Derivative on Cell Spheroids Made of Stem Cells Obtained from the Gingiva on Osteogenic Differentiation

Hwa

Lee

et al. 2023

Medicina

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“…There are few studies that address detailed morphometry, in millimeters, of this anomaly. Some of this, describe the groove in lateral incisor, on the palatal radicular face [4][5][6]17]. Brin e Ben-Bassat [3] classified the depth of the LCVG in UCI as shallow or deep but did not describe the criteria for such classification and neither quantify in millimeters how this anomaly varied within the sample.…”

Section: Discussionmentioning

confidence: 99%

Morphological and morphometric characteristics of labial-cervical-vertical groove in upper central incisor

Fabiana Cervo de Barros,

Luiza Marins de Oliveira Cortinhas,

Anne Luise Scabell Almeida

et al. 2023

RSBO

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The labial-cervical-vertical groove (LCVG) is a developmental dental anomaly that may be present in the maxillary central incisors (UCI) which can affect periodontal health, facilitate the occurrence cervical decay, and compromise the aesthetics of the gingival contour. A LCVG can present different magnitudes and, when severe, it can impact the prognosis of the affected tooth. Objective: The objective of this study was to investigate the prevalence, extent, and depth of labial-cervical-vertical groove (LCVG) in maxillary central incisors (UCI) from the Human Tooth Bank of Rio de Janeiro State University Dental School, aiming to provide a better understanding of this anomaly and thus to contribute to an accurate differential diagnosis and appropriate dental planning. Material and methods: Ninety-seven dental elements, belonging to the Human Tooth Bank of Rio de Janeiro State University Dental School, were inspected, for presence of LCVG. For analysis of this morphological alteration, the following measurements were registered in millimeters: the total length (measured from the coronal to the apical limits of the defect); its extension in the crown (measured from the defect’s coronal limit to the cementum enamel junction (CEJ)); its extension in the root (measured from the defect’s apical limit to the CEJ) and depth (measurement of the groove’s invagination toward the root canal) of LCVG in each tooth. Results: The prevalence of LCVG in our sample was 7.21%. The average measures observed for the LCVG were: 6.43 (± 1.81) mm of total of length, 2.86 (± 1.68) mm of extension in the root; 3.57 (± 0.98) mm of extension in the crown; and <1 to 1mm of depth. Conclusion: These findings contribute with knowledge on of the detail, of extent and depth of LCVG in UCI, providing the clinician with information that can favor diagnosis and maintenance of teeth with this anomaly.

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“…Recently, cases were reported to handle this condition with innovative strategies including root canal treatment followed by intentional replantation[ 3 , 18 ] and enamel matrix augmented periodontal regeneration[ 19 ], but the long-term follow-ups are necessary to illustrate the effectiveness of these cases. In this case report, under the intentional replantation procedure, the clinician could clean a vast amount of bacterial plaque, granulation tissues, and caries that were often noted on the surface of the grooves.…”

Section: Discussionmentioning

confidence: 99%

Diverse microbiota in palatal radicular groove analyzed by Illumina sequencing: Four case reports

Tan¹,

Chen²,

Fu³

et al. 2021

WJCC

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BACKGROUND A palatal radicular groove is an unusual developmental deformity of the tooth, which may serve as a channel linking the periodontal and periapical inflammation, and yet no literature could be obtained analyzing microbiota within the palatal radicular grooves. CASE SUMMARY Four patients diagnosed with palatal radicular groove and concomitant periodontal-endodontic deformity in permanent maxillary lateral incisors were enrolled in this work. Twelve bacterial samples from 4 patients were collected from different parts of the palatal radicular groove during intentional replantation surgery. Illumina sequencing was performed to analyze the taxonomical composition and microbiome structure inside the palatal grooves, and 1162 operational taxonomic units were obtained. The phyla of Firmicutes and Proteobacteria predominated in most of the samples. An unknown genus from the Bacillaceae family, Lactococcus , and Porphyromonas were the most abundant genera identified. There was no difference in the microbiota richness and diversity in three sections of the groove. CONCLUSION The unique ecological niches inside the palatal grooves harbored bacterial communities that shared some component features of both the endodontic and periodontal infections. The existence of palatal groove may play an interaction bridge between the root apex and tooth cervix and thus impair the outcome of traditional therapeutic methods such as root canal treatment and periodontal management.

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Periodontal Regenerative Treatment of Intrabony Defects Associated with Palatal Grooves: A Report of Two Cases

Cited by 8 publications

References 34 publications

Effects of Enamel Matrix Derivative on Cell Spheroids Made of Stem Cells Obtained from the Gingiva on Osteogenic Differentiation

Effects of Enamel Matrix Derivative on Cell Spheroids Made of Stem Cells Obtained from the Gingiva on Osteogenic Differentiation

Morphological and morphometric characteristics of labial-cervical-vertical groove in upper central incisor

Diverse microbiota in palatal radicular groove analyzed by Illumina sequencing: Four case reports

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