Periodontal regeneration induced by porous alpha-tricalcium phosphate with immobilized basic fibroblast growth factor in a canine model of 2-wall periodontal defects

Abstract

We evaluated the effect of porous alpha-tricalcium phosphate (α-TCP) with immobilized basic fibroblast growth factor (bFGF) on periodontal regeneration in a canine model of 2-wall periodontal defects. Identical bone defects were made in the canine mandible; six defects in each animal were filled with porous α-TCP with bFGF bound via heparin (bFGF group), and the remaining defects were filled with unmodified porous α-TCP (control group). Micro-computed tomography and histological evaluation were performed at 2, 4, and 8 weeks post-implantation. The bone mineral content of the bFGF group was higher than that of the control group at 2 and 4 weeks (p < 0.05). Histological evaluation at 2 weeks post-implantation revealed degradation of the porous α-TCP, and bone had formed on the surface of α-TCP particles in the bFGF group. Some of these collagen fibers connected the newly formed cementum with the alveolar bone, revealing the formation of new periodontal ligaments with Sharpey's fibers. At 8 weeks, continuous cortical bone with a Haversian structure covered the top of the bone defects in the bFGF group. These findings indicate that porous α-TCP with immobilized bFGF could promote periodontal regeneration at the early regeneration phase in a canine model of 2-wall periodontal defects.

Application of hydroxyapatite nanoparticle-assembled powder using basic fibroblast growth factor as a pulp-capping agent

“…Our previous study 42) demonstrated that FGF2 immobilized on alpha-tricalcium phosphate particles enhanced vascularization and could effectively reduce the time required to regenerate the periodontium in a canine model of two-walled periodontal defects. In the present study, we frequently observed ingrowth of vascular endothelial cells into the exposed pulp treated with FGF2 in the early stage (1 week after surgery), which then disappeared along with the appearance of a dentinal bridge-like structure in the late stage (2 weeks after surgery).…”

Regenerative benefits of using growth factors in treatment of periodontal defects: A systematic review and meta‐analysis with Trial Sequential Analysis on preclinical studies

“…For the studies devoid of quantitative data in their paper or if required data was only expressed graphically, an email was sent to access the exact data; where the correspondence turned out to be fruitless, the graphs in six studies (Anzai et al, 2016;Chang et al, 2013;Matsuse et al, 2018;Momose et al, 2016;Nagayasu-Tanaka et al, 2015;Ripamonti et al, 2017) were analyzed by software DigitizeIt 2.5 (DigitizeIt) to extract the numerical measurements. The desired graphs were scanned and imported to the software.…”

“…Besides, two of them reported that these biomolecules had a positive effect on PDL formation (Anzai et al, 2016;Matsuse et al, 2018).…”

In vitro and in vivo effectiveness of a novel injectable calcitonin-loaded collagen/ceramic bone substitute

“…17 On the other hand, a growing number of synthetic bone graft cements have been designed as drug delivery vehicles to release exogenous growth factor to the bone defect area, aiming to enhance osteogenesis and bone healing. [18][19][20] Calcitonin, a 32-amino acid peptide hormone produced by thyroid parafollicular cells, inhibits bone resorption by osteoclasts, thereby reducing the serum calcium level. 21 Calcitonin has been used as an antiresorptive agent to treat postmenopausal osteoporosis for decades.…”

“…17 On the other hand, a growing number of synthetic bone graft cements have been designed as drug delivery vehicles to release exogenous growth factor to the bone defect area, aiming to enhance osteogenesis and bone healing. 18–20…”