Periodontal ligament cell populations: The central role of fibroblasts in creating a unique tissue

Lekic, P.; McCulloch, Christopher A.

doi:10.1002/(sici)1097-0185(199606)245:2<327::aid-ar15>3.0.co;2-r

Cited by 305 publications

(198 citation statements)

References 125 publications

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“…The periodontal ligament is situated between the tooth and the alveolar bone, and it supports the attachment of the teeth to the alveolar bone. Furthermore mesenchymal stem cells are present in the PDL and are able to differentiate into multiple types of cells that play essential roles by responding to the mechanical forces that affect teeth and by repairing damaged matrix (24). Preferential localization of PLAP-1/asporin in the PDL suggests that it has different and unique roles in the homeostasis and function of the PDL.…”

Section: Discussionmentioning

confidence: 99%

PLAP-1/Asporin, a Novel Negative Regulator of Periodontal Ligament Mineralization

Yamada¹,

Tomoeda²,

Ozawa³

et al. 2007

Journal of Biological Chemistry

180

189

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Periodontal ligament-associated protein-1 (PLAP-1)/asporin is a recently identified novel member of the small leucine-rich repeat proteoglycan family. PLAP-1/asporin is involved in chondrogenesis, and its involvement in the pathogenesis of osteoarthritis has been suggested. We report that PLAP-1/asporin is also expressed specifically and predominantly in the periodontal ligament (PDL) and that it negatively regulates the mineralization of PDL cells. In situ hybridization analysis revealed that PLAP-1/asporin was expressed specifically not only in the PDL of an erupted tooth but also in the dental follicle, which is the progenitor tissue of the PDL during tooth development. Overexpression of PLAP-1/asporin in mouse PDL-derived clone cells interfered with both naturally and bone morphogenetic protein 2 (BMP-2)-induced mineralization of the PDL cells. On the other hand, knockdown of PLAP-1/asporin transcript levels by RNA interference enhanced BMP-2-induced differentiation of PDL cells. Furthermore co-immunoprecipitation assays showed a direct interaction between PLAP-1/asporin and BMP-2 in vitro, and immunohistochemistry staining revealed the co-localization of PLAP-1/asporin and BMP-2 at the cellular level. These results suggest that PLAP-1/ asporin plays a specific role(s) in the periodontal ligament as a negative regulator of cytodifferentiation and mineralization probably by regulating BMP-2 activity to prevent the periodontal ligament from developing non-physiological mineralization such as ankylosis.

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Section: Discussionmentioning

confidence: 99%

PLAP-1/Asporin, a Novel Negative Regulator of Periodontal Ligament Mineralization

Yamada¹,

Tomoeda²,

Ozawa³

et al. 2007

Journal of Biological Chemistry

180

189

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“…Generally, PDL includes multipopulation cells consisting mainly of fibroblasts with high alkaline phosphatase activity, 14 and fibroblasts from PDL share many physiological characteristics with osteoblasts. 15,16 Some studies already described methods to isolate PDL cells from PDL, and an almost identical method was used in this study.…”

Section: Discussionmentioning

confidence: 99%

Effects of compressive stress on the expression of M-CSF, IL-1β, RANKL and OPG mRNA in periodontal ligament cells

et al. 2009

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Objective: The aim of this study was to determine if human PDL cells can produce osteoclastogenic mRNA and examine how compressive stress affects the expression of osteoclastogenic mRNA in human PDL cells. Methods: Human PDL cells were obtained from biscupids extracted for orthodontic treatment. The compressive force was adjusted by increasing the number of cover glasses. PDL cells were subjected to a compressive force of 0.

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“…1,3 Tooth movement only occurs when the areas of hyalinized (cell-free) tissue in the compression side, created due to a combination of inflammatory cytokines and a disruption in the blood flow 1,4 are removed. 1,2,4 Removal of this tissue and adjacent alveolar bone is achieved by circulating macrophages and bone marrow osteoclastic precursors that are recruited to the periodontal ligament (PDL).…”

Section: Introductionmentioning

confidence: 99%

Molecular Markers of Early Orthodontic Tooth Movement

Brooks

Nilforoushan

Manolson

et al. 2009

The Angle Orthodontist

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Objective: To understand the molecular basis of early orthodontic tooth movement by looking at the expression of KI-67, runt-related transcription factor 2 (Runx2), and tumor necrosis factor ligand superfamily member 11 (RANKL) proteins. Materials and Methods:We employed a rat model of early orthodontic tooth movement using a split-mouth design (where contralateral side serves as a control) and performed immunohistochemical staining to map the spatial expression patterns of three proteins at 3 and 24 hours after appliance insertion. Results: We observed increased expression of KI-67, a proliferation marker, and RANKL, a molecule associated with osteoclastic differentiation, in the compression sites of the periodontal ligament subjected to 3 hours of force. In contrast, there was increased expression of KI-67 and Runx2, a marker of osteoblast precursors, in tension areas after 24 hours of force. Decreased KI-67 expression in the mesial and distal regions of the periodontal ligament was observed at the midpoint of the tooth root. Conclusions:The early RANKL expression indicates that at this early stage cells are involved in osteoclast precursor signaling. Also, decreased KI-67 expression found near the midpoint of the tooth root is believed to represent the center of rotation, providing a molecular means of visualizing mechanical loading patterns.

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Periodontal ligament cell populations: The central role of fibroblasts in creating a unique tissue

Cited by 305 publications

References 125 publications

PLAP-1/Asporin, a Novel Negative Regulator of Periodontal Ligament Mineralization

PLAP-1/Asporin, a Novel Negative Regulator of Periodontal Ligament Mineralization

Effects of compressive stress on the expression of M-CSF, IL-1β, RANKL and OPG mRNA in periodontal ligament cells

Molecular Markers of Early Orthodontic Tooth Movement

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