Periodic acid-Schiff (PAS) reaction and plastination in whole body slices. A novel technique to identify fascial tissue structures

Steinke, Hanno; Wiersbicki, Dina; Speckert, Marie-Lynn; Merkwitz, Claudia; Wolfskämpf, Thomas; Wolf, Benjamin

doi:10.1016/j.aanat.2017.10.001

Cited by 18 publications

(9 citation statements)

References 21 publications

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“…Recently, using a novel slicing and staining technique, Steinke et al . have shown dense, parallel aligned collagen layers between abutting pelvic tissue complexes such as the uterine cervix and the rectum or urinary bladder 40 . These collagen layers may halt locally invading tumour cells.…”

Section: Discussionmentioning

confidence: 99%

Roadmap to Local Tumour Growth: Insights from Cervical Cancer

Kubitschke

Wolf

Morawetz

et al. 2019

Sci Rep

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Wide tumour excision is currently the standard approach to surgical treatment of solid cancers including carcinomas of the lower genital tract. This strategy is based on the premise that tumours exhibit isotropic growth potential. We reviewed and analysed local tumour spreading patterns in 518 patients with cancer of the uterine cervix who underwent surgical tumour resection. Based on data obtained from pathological examination of the surgical specimen, we applied computational modelling techniques to simulate local tumour spread in order to identify parameters influencing preferred infiltration patterns and used area-proportional Euler diagrams to detect and confirm ordered patterns of tumour spread. Some anatomical structures, e.g. tissues of the urinary bladder, were significantly more likely to be infiltrated than other structures, e.g. the ureter and the rectum. Computational models assuming isotropic growth could not explain these infiltration patterns. Introducing ontogenetic distance of a tissue relative to the uterine cervix as a parameter led to accurate predictions of the clinically observed infiltration likelihoods. The clinical data indicates that successive infiltration likelihoods of ontogenetically distant tissues are nearly perfect subsets of ontogenetically closer tissues. The prevailing assumption of isotropic tumour extension has significant shortcomings in the case of cervical cancer. Rather, cervical cancer spread seems to follow ontogenetically defined trajectories.

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Section: Discussionmentioning

confidence: 99%

Roadmap to Local Tumour Growth: Insights from Cervical Cancer

Kubitschke

Wolf

Morawetz

et al. 2019

Sci Rep

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“…Plastinates were used to reveal the layout of the FN fascia plate. We used a standard plastination method and staining with periodic acid‐Schiff (PAS) reaction as further described (Steinke et al, 2018). For additional quantitative insight we plastinated the dissection of case 19 (Steinke, 2001).…”

Section: Methodsmentioning

confidence: 99%

“…We used a standard plastination method and staining with periodic acid-Schiff (PAS) reaction as further described (Steinke et al, 2018).…”

Section: Histology/submacroscopymentioning

confidence: 99%

Topography and evidence of a separate “fascia plate” for the femoral nerve inside the iliopsoas – A dorsal approach

et al. 2020

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“…For the staining, we employed a macroscopic protocol that uses the periodic acid-Schiff reaction with subsequent plastination to distinguish collagen types (red to pink; Steinke et al, 2018) from muscle (unstained, ochre;Mulisch and Welsch, 2015). A red precipitation demarcates Type I collagen and a pink color stains Type II collagen.…”

Section: Plastination and Stainingmentioning

confidence: 99%

“…A red precipitation demarcates Type I collagen and a pink color stains Type II collagen. After the reaction, we transferred the slices to pure acetone which was renewed daily until the slices contained <2% water (Steinke et al, 2018).…”

Section: Plastination and Stainingmentioning

confidence: 99%

The fascial connections of the pectineal ligament

et al. 2019

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In clinical settings, the pectineal ligament forms a basic landmark for surgical approaches. However, to date, the detailed fascial topography of this ligament is not well understood. The aim of this study was to describe the morphology of the pectineal ligament including its fascial connections to surrounding structures. The spatial-topographical relations of 10 fresh and embalmed specimens were dissected, stained, slice plastinated, and analyzed macroscopically, and in three cases histological approaches were also used. The pectineal ligament is attached ventrally and superiorly to the pectineus muscle, connected to the inguinal ligament by the lacunar ligament and to the tendinous origin of rectus abdominis muscle and the iliopubic tract. It forms a site of origin for the internal obturator muscle, and throughout its curved course, the ligament attaches to both the fasciae of iliopsoas and the internal obturator muscle. However, dorsally, these fasciae pass free from the bone, while the pectineal ligament itself is adhered to it. The organ fasciae are seen apart from the pectineal ligament and its connections. The pectineal ligament seems to form a connective tissue junction between the anterior and medial compartment of the thigh. This ligament, however, is free to other compartments arisen from the embryonal gut and to the urogenital ridge. These features of the pectineal ligament are important to consider during orthopedic and trauma surgical approaches, in gynecology, hernia and incontinence surgery, and in operations for pelvic floor and neovaginal reconstructions. Clin. Anat. 32:961-969, 2019.

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Periodic acid-Schiff (PAS) reaction and plastination in whole body slices. A novel technique to identify fascial tissue structures

Cited by 18 publications

References 21 publications

Roadmap to Local Tumour Growth: Insights from Cervical Cancer

Roadmap to Local Tumour Growth: Insights from Cervical Cancer

Topography and evidence of a separate “fascia plate” for the femoral nerve inside the iliopsoas – A dorsal approach

The fascial connections of the pectineal ligament

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