Perinatal Zearalenone Exposure Affects RXFP1, RXFP2, and Morphoregulatory Gene Expression in the Neonatal Porcine Uterus

Chen, Joseph C.; Wiley, Anne A.; Kauffold, Johannes; Wähner, M.; Bartol, Frank F.; Bagnell, Carol A.

doi:10.1111/j.1749-6632.2008.03815.x

Cited by 5 publications

(5 citation statements)

References 6 publications

(6 reference statements)

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“…RXFP1 gene encoding for relaxin receptor was the most significantly down-regulated (0.25 Fc); this receptor activates a wide spectrum of signalłing pathways and induces the activation of several G protein-coupled receptors to produce important messengers like cAMP and nitric oxide [ 44 ]. Also, RXFP1 is able to activate Erk1/2 tyrosine kinase [ 44 ] an estrogenic-sensitive MAP-kinase and this could explain the effect of ZEA as estrogenic disruptor [ 45 ]. Indeed, uterine RXFP1 transcripts were decreased in piglets as a consequence of continuous ZEA exposure [ 45 ].…”

Section: Discussionmentioning

confidence: 99%

“…Also, RXFP1 is able to activate Erk1/2 tyrosine kinase [ 44 ] an estrogenic-sensitive MAP-kinase and this could explain the effect of ZEA as estrogenic disruptor [ 45 ]. Indeed, uterine RXFP1 transcripts were decreased in piglets as a consequence of continuous ZEA exposure [ 45 ]. The most up-regulated signalłing gene was OR10J3, involved in innate immunity and associated with risk for breast cancer in women [ 46 ].…”

Section: Discussionmentioning

confidence: 99%

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Zearalenone Mycotoxin Affects Immune Mediators, MAPK Signalling Molecules, Nuclear Receptors and Genome-Wide Gene Expression in Pig Spleen

et al. 2015

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The toxicity of zearalenone (ZEA) was evaluated in swine spleen, a key organ for the innate and adaptative immune response. Weaned pigs were fed for 18 days with a control or a ZEA contaminated diet. The effect of ZEA was assessed on wide genome expression, pro- (TNF-α, IL-8, IL-6, IL-1β, IFN-γ) and anti-inflammatory (IL-10, IL-4) cytokines, other molecules involved in inflammatory processes (MMPs/TIMPs), as well as signaling molecules, (p38/JNK1/JNK2-MAPKs) and nuclear receptors (PPARγ/NFkB/AP-1/STAT3/c-JUN). Microarray analysis showed that 46% of total number of differentially expressed genes was involved in cellular signaling pathway, 13% in cytokine network and 10% in the inflammatory response. ZEA increased expression and synthesis of pro- inflammatory (TNF-α, IL-8, IL-6, IL-1β) and had no effect on IFN-γ, IL-4 and IL-10 cytokines in spleen. The inflammatory stimulation might be a consequence of JNK pathway activation rather than of p-38MAPK and NF-kB involvement whose gene and protein expression were suppressed by ZEA action. In summary, our findings indicated the role of ZEA as an immune disruptor at spleen level.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Zearalenone Mycotoxin Affects Immune Mediators, MAPK Signalling Molecules, Nuclear Receptors and Genome-Wide Gene Expression in Pig Spleen

et al. 2015

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“…Significant increases in degeneration were seen at concentrations > 30 μM for both α-zearalenol and β-zearalenol. Chen et al (2009) studied the effects of maturing pig oocytes in vitro in the presence of zearalenone or its metabolites, α-and β-zearalenol at concentrations of 0, 0.312, 3.12 or 31.2 μmol/L). The number of zygotes examined at each concentration ranged from 29-32.…”

Section: In Vitro Studiesmentioning

confidence: 99%

“…The effects of in vivo perinatal zearalenone exposure on the expression of oestrogen-sensitive, uterine genes associated with endometrial development in neonatal female pigs were studied (Chen et al, 2009). The genes were relaxin/insulin-like factor 3 receptors RXFP1 and RXFP2, the morphoregulatory genes Wnt4, Wnt7a and Hoxa10, and ER-α.…”

Section: In Vivo Studiesmentioning

confidence: 99%

“…This study also illustrates the stimulatory and inhibitory effects of a zearalenone metabolite on steroidogenesis in porcine cells in culture, dependent on the concentration of the test substance and the culture conditions. Chen et al (2009) studied the effects of maturing pig oocytes in vitro in the presence of zearalenone or its metabolites, α-and β-zearalenol at concentrations of 0, 0.312, 3.12 or 31.2 μmol/L). They caused a concentration-related inhibition of oocyte, maturation abnormal formation of aberrant spindle and abnormal nuclei.…”

Section: In Vitro Studiesmentioning

confidence: 99%

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Scientific Opinion on the risks for public health related to the presence of zearalenone in food

2011

EFSA Journal

346

142

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Zearalenone is a mycotoxin produced by several Fusarium species. It is commonly found in maize but can be found also in other crops such as wheat, barley, sorghum and rye. The European Commission asked the European Food Safety Authority to review the safety of zearalenone and the risk to consumers of a possible increase of the maximum level (ML) for zearalenone in breakfast cereals. A total of 13,075 analytical results obtained on food samples and 9,877 results on unprocessed grains sampled by 19 European countries in 2005-2010 were used in the evaluation. The highest concentrations of zearalenone were reported for wheat bran, corn and products thereof (e.g. corn flour, cornflakes). Grains and grain-based foods, in particular grains and grain milling products, bread and fine bakery wares, made the largest contribution to the estimated zearalenone exposures. Vegetable oils also made an important contribution to the zearalenone exposure. The critical effects of zearalenone result from its oestrogenic activity. Based on recent data in the most sensitive animal species, the pig, and taking into account comparisons between pigs and humans, the Panel on Contaminants in the Food Chain established a tolerable daily intake (TDI) for zearalenone of 0.25 μg/kg b.w. Estimates of chronic dietary exposure to zearalenone based on the available occurrence data are below or in the region of the TDI for all age groups and not a health concern. A potential increase in the ML for zearalenone in breakfast cereals from 50 µg/kg to 75, 100, 125 or 150 µg/kg is unlikely to result in a chronic dietary exposure exceeding the TDI. In a worst case scenario it is possible that an individual could consume the same batch of breakfast cereal containing zearalenone at the ML every day for 2 to 4 weeks, in which case exposures may exceed the TDI.

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Protective effect of glucosamine on zearalenone-induced reproductive toxicity and placental dysfunction in mice

Bai¹,

Hai²,

Zhai³

et al. 2023

Food and Chemical Toxicology

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Perinatal Zearalenone Exposure Affects RXFP1, RXFP2, and Morphoregulatory Gene Expression in the Neonatal Porcine Uterus

Cited by 5 publications

References 6 publications

Zearalenone Mycotoxin Affects Immune Mediators, MAPK Signalling Molecules, Nuclear Receptors and Genome-Wide Gene Expression in Pig Spleen

Zearalenone Mycotoxin Affects Immune Mediators, MAPK Signalling Molecules, Nuclear Receptors and Genome-Wide Gene Expression in Pig Spleen

Scientific Opinion on the risks for public health related to the presence of zearalenone in food

Protective effect of glucosamine on zearalenone-induced reproductive toxicity and placental dysfunction in mice

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