Perinatal protein restriction affects milk free amino acid and fatty acid profile in lactating rats: potential role on pup growth and metabolic status

Agnoux, Aurore Martin; Antignac, Jean‐Philippe; Boquien, Clair‐Yves; David, Anne; Desnots, Emmanuelle; Ferchaud‐Roucher, Véronique; Parnet, Patricia; Alexandre-Gouabau, Marie Cécile

doi:10.1016/j.jnutbio.2015.02.012

Cited by 38 publications

(17 citation statements)

References 65 publications

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“…Additionally, most of the EAAs and NEAAs, such as Ile, Lys, Val, Ala, Asp, Glu, Ser and Tyr, increased significantly in the maternal and fetal plasma in the 25% Met-Met group compared to those in the 35% Met group ( Table S6 ). Moreover, the sum of the AAs reported to promote insulin secretion (i.e., Val + Leu + Ile + Lys + Thr + Arg) [ 20 ] was lower in the maternal and fetal plasma of the 35% Met group ( p < 0.001) than that in the 25% Met-Met group ( Figure 4 C), so did the branched-chain AAs (Val + Leu + Ile; Figure 4 D). Conversely, the concentrations of the sulfur-containing FAAs (i.e., Met + Cys) were significantly lower in the dams in the 25% Met-Met group ( p < 0.001) than those in the 35% Met group ( Figure 4 B).…”

Section: Resultsmentioning

confidence: 99%

Methionine Partially Replaced by Methionyl-Methionine Dipeptide Improves Reproductive Performance over Methionine Alone in Methionine-Deficient Mice

et al. 2018

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Decreased protein breakdown in pregnant women results in lower concentration of methionine (Met) in plasma, causing pregnancy-related metabolic disturbance. Its dipeptide methionyl-methionine (Met-Met) may exert positive influence in fetal development. This study mainly investigated whether Met-Met can be used as part of free Met to promote reproductive outcomes in mice and the underlying mechanisms. Met-deficient pregnant mice were treated with Met alone or with Met-Met during pregnancy. Daily intraperitoneal injection of 35% dietary Met in pregnant mice was the best dose among the 15–45% doses. Embryo development and newborn birth weight were enhanced when 25% of the Met in the 35% Met group was replaced with Met-Met. Met-Met replacement had higher plasma insulin, glucose, and free amino acids (AA) concentrations. Besides, in the placenta, the AA transporter mRNA abundances and peptide transporters (PhT1 and PepT1) protein levels were higher in Met-Met treatment group. Moreover, Met-Met increased 4E-BP1, S6K1 and AKT/mTOR phosphorylation. These results suggest that Met-Met could be used as a partial source of Met to promote reproductive outcomes in Met-restricted pregnant mice, which might be mediated by promoting nutrient availability and activating AKT/mTOR-mediated signaling pathway.

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Section: Resultsmentioning

confidence: 99%

Methionine Partially Replaced by Methionyl-Methionine Dipeptide Improves Reproductive Performance over Methionine Alone in Methionine-Deficient Mice

et al. 2018

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“…Additionally, maternal protein restriction was associated with changes in maternal milk nutrients. The levels of amino acids involved in gluconeogenesis decreased and the total fatty acid level rose [24]. Fulfilment of the energy requirements of the offspring by milk affected by poor maternal nutrition may play roles in offspring growth and metabolic programming.…”

Section: Discussionmentioning

confidence: 99%

Maternal Food Restriction during Pregnancy and Lactation Adversely Affect Hepatic Growth and Lipid Metabolism in Three-Week-Old Rat Offspring

Lee

You

Kwon

et al. 2016

IJMS

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Maternal malnutrition influences the early development of foetal adaptive changes for survival. We explored the effects of maternal undernutrition during gestation and lactation on hepatic growth and function. Sprague-Dawley rats were fed a normal or a food-restricted (FR) diet during gestation and/or lactation. We performed analyses of covariance (adjusting for the liver weight/body weight ratio) to compare hepatic growth and lipid metabolism among the offspring. Maternal FR during gestation triggered the development of wide spaces between hepatic cells and increased the expression of mammalian target of rapamycin (mTOR) in three-week-old male offspring compared with controls (both p < 0.05). Offspring nursed by FR dams exhibited wider spaces between hepatic cells and a lower liver weight/body weight ratio than control offspring, and increased mTOR expression (p < 0.05). Interestingly, the significant decrease in expression of lipogenic-related genes was dependent on carbohydrate-responsive element-binding protein, despite the increased expression of sterol regulatory element-binding protein 1 (SREBP1) (p < 0.05). This study demonstrated increased expression of key metabolic regulators (mTOR and SREBP1), alterations in lipid metabolism, and deficits in hepatic growth in the offspring of FR-treated dams.

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“…Two lipid analyses were achieved: a first targeted analysis of total fatty acids and a second untargeted lipidomic profiling. Total fatty acids were analyzed by gas chromatography using an Agilent Technologies 7890A ® instrument (Perkin Elmer, Villebon-sur-Yvette, France), following trans-esterification [ 46 ]. See Supplementary Materials online for detailed information about biochemical analysis and materials used.…”

Section: Methodsmentioning

confidence: 99%

Breast Milk Lipidome Is Associated with Early Growth Trajectory in Preterm Infants

et al. 2018

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Human milk is recommended for feeding preterm infants. The current pilot study aims to determine whether breast-milk lipidome had any impact on the early growth-pattern of preterm infants fed their own mother’s milk. A prospective-monocentric-observational birth-cohort was established, enrolling 138 preterm infants, who received their own mother’s breast-milk throughout hospital stay. All infants were ranked according to the change in weight Z-score between birth and hospital discharge. Then, we selected infants who experienced “slower” (n = 15, −1.54 ± 0.42 Z-score) or “faster” (n = 11, −0.48 ± 0.19 Z-score) growth; as expected, although groups did not differ regarding gestational age, birth weight Z-score was lower in the “faster-growth” group (0.56 ± 0.72 vs. −1.59 ± 0.96). Liquid chromatography–mass spectrometry lipidomic signatures combined with multivariate analyses made it possible to identify breast-milk lipid species that allowed clear-cut discrimination between groups. Validation of the selected biomarkers was performed using multidimensional statistical, false-discovery-rate and ROC (Receiver Operating Characteristic) tools. Breast-milk associated with faster growth contained more medium-chain saturated fatty acid and sphingomyelin, dihomo-γ-linolenic acid (DGLA)-containing phosphethanolamine, and less oleic acid-containing triglyceride and DGLA-oxylipin. The ability of such biomarkers to predict early-growth was validated in presence of confounding clinical factors but remains to be ascertained in larger cohort studies.

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Perinatal protein restriction affects milk free amino acid and fatty acid profile in lactating rats: potential role on pup growth and metabolic status

Cited by 38 publications

References 65 publications

Methionine Partially Replaced by Methionyl-Methionine Dipeptide Improves Reproductive Performance over Methionine Alone in Methionine-Deficient Mice

Methionine Partially Replaced by Methionyl-Methionine Dipeptide Improves Reproductive Performance over Methionine Alone in Methionine-Deficient Mice

Maternal Food Restriction during Pregnancy and Lactation Adversely Affect Hepatic Growth and Lipid Metabolism in Three-Week-Old Rat Offspring

Breast Milk Lipidome Is Associated with Early Growth Trajectory in Preterm Infants

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