Perinatal outcome of twice-frozen-thawed embryo transfers: a clinical follow-up study

Murakami, Masao; Egashira, A.; Murakami, Kimiko; Araki, Yasuhisa; Kuramoto, Takeshi

doi:10.1016/j.fertnstert.2010.12.038

Cited by 36 publications

(37 citation statements)

References 16 publications

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“…Therefore, repeated cryopreservation procedures for surplus embryos should be considered. To our knowledge, limited data are available for FET outcome with twice-cryopreserved human embryos, and most are case reports (Baker et al, 1996;Check et al, 2001;Estes et al, 2003;Farhat et al, 2001;Murakami et al, 2011;Sills et al, 2009). Lierman et al (2014) used spare poorquality human embryos as material and indicated that there are no differences in terms of survival and re-expansion status between twicecryopreserved and once-vitrified blastocysts.…”

Section: Introductionmentioning

confidence: 99%

Effect of repeated cryopreservation on human embryo developmental potential

Zheng

Yuan

Yan

et al. 2017

Reproductive BioMedicine Online

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Repeated cryopreservation of surplus embryos from frozen-thawed cycles should occasionally be considered. The purpose of this retrospective cohort study was to evaluate the pregnancy and perinatal outcome of repeated cryopreservation by vitrification of human blastocysts derived from slowly frozen-thawed day 3 embryos. In total, 571 vitrified-warmed blastocyst transfer cycles were investigated. The vitrified-warmed blastocysts were derived from slowly frozen-thawed cleavage embryos (twice-cryopreserved group) or fresh embryos (control group) cultured to the blastocyst stage. Age, body mass index, endometrial thickness, blastocyst developmental rate and number of embryos transferred were not significantly different between twice-cryopreserved and control groups. Clinical pregnancy and implantation rates were also similar. Compared with controls, the miscarriage rate was significantly higher in the twice-cryopreserved group (33.93% versus 19.07%, P = 0.017). This resulted in a significantly lower live birth rate in the twice-cryopreserved group than in controls (29.13% versus 39.18, P = 0.038). No differences were observed in mean gestational age, birthweight and sex ratio of newborns between groups. In conclusion, acceptable clinical pregnancy outcomes may be expected from transfer of twice-cryopreserved human embryos. While the neonatal outcome is not affected, the correlation between the risk of higher pregnancy loss and repeated cryopreservation needs further investigation.

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Section: Introductionmentioning

confidence: 99%

Effect of repeated cryopreservation on human embryo developmental potential

Zheng

Yuan

Yan

et al. 2017

Reproductive BioMedicine Online

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“…21 Moreover, other studies have shown that vitrifi cation offers the unique opportunity to submit a vitrifi ed and warmed embryo to subsequent vitrifi cation without affecting its viability. 25,26 In summary, this comparison proves vitrifi cation to be an option that is clearly cheaper, faster and safer as to effects on metabolism and mechanical damage, and clinical results are impressive. Essentially, the only points where traditional slow cryopreservation outweighs the method of vitrifi cation concern the risk of contamination and toxicity.…”

Section: Vitrification Versus Slow-freezingmentioning

confidence: 74%

Oocyte And Embryo Vitrification In The Ivf Laboratory: A Comprehensive Review

Simopoulou¹,

Asimakopoulos²,

Bakas³

et al. 2014

Folia Medica

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Vitrifi cation is an excellent tool in the IVF laboratory, enabling options and offering fl exibility in assisted reproduction. The technology of cryopreservation has been underway since the early 20 th century. The advent of vitrifi cation has advanced the expectations in routine clinical practice in the IVF laboratory presenting impressive results both in post-thaw survival, and in clinical pregnancy rates, as well as signifi cantly enhancing clinical results on preimplantation genetic diagnosis (PGD). Contradicting opinions have been published recently on the limitations and potential that vitrifi cation has in the laboratory, as well as on the optimal approach to employ vitrifi cation in IVF. This review aims to present a comprehensive analysis of the practical aspects of vitrifi cation including concerns and options regarding its use on oocytes and embryos while comparing it with the traditional "slow-freezing" cryopreservation technique. РЕЗЮМЕТехнология криоконсервации применяется с начала ХХ в. Открытие технологии витрификации повысило ожидания в области утвержденных клинических деятельностей в лабораториях по ин-витро фертилизации, привело к отличным результатам, как в отношении выживания после размораживания и количества беременностей, так и в отношении значительного улучшения клинических результатов преимплантационной генетической диагностики. В последнее время появились противоречивые утверждения относительно потенциала и ограничения применения витрификации в лабораторных условиях, а также относительно оптимального применения витрификации в области методик ин-витро фертилизации. Целью данной работы является предоставление подробного анализа практических сторон витрификации, в том числе и возможных рисков и вариантов её применения в отношении ооцитов и эмбрионов, по сравнению с традиционными методиками криоконсервации способом "медленного замораживания". В заключение можно сказать, что для лабораторий ин-витро фертилизации витрификация является отличным средством, которое предоставляет возможности выбора и гибкости в области искусственного оплодотворения.Ключeвые слова: ооцит, эмбрион, витрификация, криоконсервация, ин-витро фертилизация Folia Medica 2014; 56(3): 161-169

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“…Kumasako et al (13) found that the implantation rate of embryos that had been vitrified at both pronuclear and blastocyst stage was comparable to that of the once-cryopreserved embryos. The research from Murakami et al (14) showed that embryos that were frozen at cleavage stage and revitrified at blastocyst stage retained high viability and resulted in normal live births. A few case studies of recryopreservation of blastocysts have been reported.…”

Section: Discussionmentioning

confidence: 99%