Perillyl alcohol (PA) mitigates inflammatory, oxidative, and histopathological consequences of allergic asthma in rats

Bejeshk, Mohammad Abbas; Beik, Ahmad; Aminizadeh, Amir Hashem; Salimi, Fouzieh; Bagheri, Fariborz; Sahebazzamani, Maryam; Najafipour, Hamid; Rajizadeh, Mohammad Amin

doi:10.1007/s00210-023-02398-5

Cited by 13 publications

(7 citation statements)

References 39 publications

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“…The membrane was placed in a sufficient amount of chromogenic substrate (TMB) solution until the bands appeared. The reaction was stopped by adding distilled water and evaluated after the bands appeared 41 – 44 .…”

Section: Methodsmentioning

confidence: 99%

Adiponectin receptor 1 could explain the sex differences in molecular basis of cognitive improvements induced by exercise training in type 2 diabetic rats

Rajizadeh,

Moslemizadeh,

Hosseini

et al. 2023

Sci Rep

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Adipokines dysregulation, the main reason for cognitive impairments (CI) induced by diabetes, shows a sex-dependent pattern inherently and in response to exercise. This study aimed to compare the attenuating effect of 8-week high intensity-interval training (HIIT) on type 2 diabetes (T2D)-induced CI between male and female rats with a special focus on adiponectin and leptin. 28 male & 28 female Wistar rats with an average age of 8 weeks were randomly assigned into four groups: control (Con), exercise (EX), Diabetes (T2D), and Type 2 diabetes + exercise (T2D + Ex). Rats in EX and T2D + EX groups performed HIIT for eight weeks (80–100% Vmax, 4–10 intervals). T2D was induced by 2 months of a high-fat diet and a single dose of STZ (35 mg/kg) administration. Leptin and adiponectin levels in serum were measured along with hippocampal expression of leptin and adiponectin receptors, AMP-activated protein kinase (AMPK), dephosphorylated glycogen synthase kinase-3 beta (Dep-GSK3β), Tau, and beta-amyloid (Aβ). Homeostasis model assessments (HOMAs) and quantitative insulin-sensitivity check index (QUICKI) indices were calculated. Our results showed that following T2D, serum levels of APN, and hippocampal levels of adiponectin receptor 1 (APNR1) were higher and HOMA-IR was lower in female than male rats (P < 0.05). However, after 8 weeks of HIIT, hippocampal levels of APNR1 and AMPK as well as QUICKI were lower and hippocampal levels of GSK, Tau, and Aβ were higher in females compared to male rats (P < 0.05). While the risk of CI following T2D was more in male than female rats HIIT showed a more ameliorating effect in male animals with APN1 as the main player.

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Section: Methodsmentioning

confidence: 99%

Adiponectin receptor 1 could explain the sex differences in molecular basis of cognitive improvements induced by exercise training in type 2 diabetic rats

Rajizadeh,

Moslemizadeh,

Hosseini

et al. 2023

Sci Rep

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“…The lavage fluid was collected by slow manual aspiration with a syringe. The collected volume, which included 75–80% instilled saline, was centrifuged (10 min, 4°C, 1000 g ), and the supernatant was used for measuring the levels of inflammatory and oxidative stress factors (Bejeshk, Aminizadeh et al., 2023 ; Bejeshk, Beik et al., 2023 ; Rajizadeh, Nematollahi et al., 2023 ).…”

Section: Methodsmentioning

confidence: 99%

Comparison of preventive and therapeutic effects of continuous exercise on acute lung injury induced with methotrexate

Rajizadeh,

Hosseini,

Bahrami

et al. 2023

Experimental Physiology

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Methotrexate (Mtx) is used to treat various diseases, including cancer, arthritis and other rheumatic diseases. However, it induces oxidative stress and pulmonary inflammation by stimulating production of reactive oxygen species and cytokines. Considering the positive effects of physical activity, our goal was to investigate the preventive and therapeutic role of continuous training (CT) on Mtx‐induced lung injury in rats. The rats were divided into five groups of 14 animals: a control group (C); a continuous exercise training group (CT; healthy rats that experienced CT); an acute lung injury with Mtx group (ALI); a pretreatment group with CT (the rats experienced CT before ALI induction), and a post‐treatment group with CT (the rats experienced CT after ALI induction). One dose of 20 mg/kg Mtx intraperitoneal was administered in the Mtx and training groups. Forty‐eight hours after the last exercise session all rats were sacrificed. According to our results, the levels of tumour necrosis factor‐α (TNF‐α), malondialdehyde (MDA), myeloperoxidase (MPO), GATA binding protein 3 (GATA3) and caspase‐3 in the ALI group significantly increased compared to the control group, and the levels of superoxide dismutase (SOD), glutathione peroxidase (GPX), total antioxidant capacity (TAC), interleukin‐10 (IL‐10), forkhead box protein 3 (FOXP3), and T‐bet decreased. In contrast, compared to the acute lung injury group, pretreatment and treatment with CT reduced TNF‐α, MDA, MPO, GATA3 and caspase‐3 and increased SOD, GPX, TAC, IL‐10, FOXP3 and T‐bet levels. The effects of CT pretreatment were more significant than the effects of CT post‐treatment. Continuous exercise training effectively reduced oxidative stress and inflammatory cytokines and ameliorated Mtx‐induced injury, and the effects of CT pretreatment were more significant than the effects of CT post‐treatment.New Findings What is the central question of this study? Considering the high prevalence of lung injury in society, does exercise as a non‐pharmacological intervention have ameliorating effects on lung injury? What is the main finding and its importance? Exercise can have healing effects on the lung after pulmonary injury through reducing inflammation, oxidative stress and apoptosis. Considering the lower side effects of exercise compared to drug treatments, the results of this study may be useful in the future.

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“…The collected volume, which included 75-80% instilled PBS, was centrifuged (10 min, 4°C, 1,000 g), and the supernatant was kept at −80°C and used for measuring the levels of inflammatory and OS factors. A part of the right lung was kept at −80°C for inflammatory and oxidant evaluation in tissue [28][29][30].…”

Section: Preparation Of Balfmentioning

confidence: 99%

Inhalation of Spray-Dried Extract of Salvia rosmarinus Spenn Alleviates Lung Inflammatory, Oxidative, and Remodeling Changes in Asthmatic Rats

Rajizadeh,

Bejeshk,

Aminizadeh

et al. 2023

Pharmacology

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Introduction: For centuries, Salvia rosmarinus Spenn has been applied as folk medicine to cure different diseases due to its anti-inflammatory, antibacterial, antioxidant, antifungal, and antitumor effects. To find bioactive medicinal herbs exerting a protective effect on airway inflammation and remodeling, we assessed the anti-oxidative and anti-inflammatory effects of an aqueous spray-dried extract of Salvia rosmarinus Spenn. (rosemary) in an ovalbumin-induced asthmatic rat model. Methods: Rats were randomly divided into normal control (control), asthma, asthma+rosemary extract (RE) (13 mg/kg), asthma+RE (50 mg/kg), and asthma+budesonide groups. After 50 days, animals were anesthetized, and then blood, bronchoalveolar lavage fluid (BALF), and lung tissues were collected for subsequent serological and pathological studies. Histopathology of lung tissues was evaluated by H&E staining. The oxidative stress parameters and airway inflammation factors in BALF and lung tissue were explored. Results: Using thin layer chromatography, the presence of rosmarinic acid was confirmed in aqueous extract of rosemary. Furthermore, RE markedly decreased immunoglobulin E levels (50 mg/kg; p < 0.001 vs. asthma group) and inflammatory cytokines (50 mg/kg; p < 0.001 vs. asthma group) and increased antioxidant enzymes (50 mg/kg, p < 0.001 vs. asthma group). Furthermore, RE at a concentration of 50 mg/kg obviously reduced the number of inflammatory cells, goblet cells, and pathological changes compared to the asthma group. Conclusion: The results showed that RE administration might prevent or alleviate allergic asthma-related pathological change, probably via antioxidant and anti-inflammatory mechanisms.

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Perillyl alcohol (PA) mitigates inflammatory, oxidative, and histopathological consequences of allergic asthma in rats

Cited by 13 publications

References 39 publications

Adiponectin receptor 1 could explain the sex differences in molecular basis of cognitive improvements induced by exercise training in type 2 diabetic rats

Adiponectin receptor 1 could explain the sex differences in molecular basis of cognitive improvements induced by exercise training in type 2 diabetic rats

Comparison of preventive and therapeutic effects of continuous exercise on acute lung injury induced with methotrexate

Inhalation of Spray-Dried Extract of <i>Salvia rosmarinus Spenn</i> Alleviates Lung Inflammatory, Oxidative, and Remodeling Changes in Asthmatic Rats

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