Perilla extract improves frequent urination in spontaneously hypertensive rats with enhancement of the urothelial presence and anti‐inflammatory effects

Kitamura, Naoya; Nishino, Machiko; Fujii, Akihiko; Hashizume, Kohjiro; Nakamura, Junji; Kondo, Hidehiko; Ohuchi, Atsushi; Hase, Tadashi; Murase, Takatoshi

doi:10.1111/iju.13516

Cited by 3 publications

(7 citation statements)

References 31 publications

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“…Several studies have reported an association between urothelial barrier dysfunction and inflammation-related LUTD. Spontaneously hypertensive rats (SHRs), which is a rat model of OAB, showed bladder inflammation and impaired urothelial barrier function with a decreased number of UPs [ 18 ]. In the SHRs, supporting urothelial barrier function improved frequent urination by reducing inflammation [ 18 ].…”

Section: Discussionmentioning

confidence: 99%

“…Spontaneously hypertensive rats (SHRs), which is a rat model of OAB, showed bladder inflammation and impaired urothelial barrier function with a decreased number of UPs [ 18 ]. In the SHRs, supporting urothelial barrier function improved frequent urination by reducing inflammation [ 18 ]. Previous studies showed increased inflammatory cells and decreased UPs in OAB patients compared to healthy individuals [ 34 , 35 ].…”

Section: Discussionmentioning

confidence: 99%

“…MCs may cause bladder hyperactivity through the activation of increased PAR2 related to MCT. Recently, some studies reported that bladder inflammation including MC infiltration, which might increase PAR2 expression, was affected by the change in Uroplakin (UP) [ 17 , 18 ]. UP Ia, UP Ib, UP II and UP III, which are transmembrane proteins in the apical membrane of the bladder urothelial cells, act as a urothelial exceptional barrier to water, small non-electrolytes and protons [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

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Involvement of Mast-Cell-Tryptase- and Protease-Activated Receptor 2—Mediated Signaling and Urothelial Barrier Dysfunction with Reduced Uroplakin II Expression in Bladder Hyperactivity Induced by Chronic Bladder Ischemia in the Rat

Akaihata

Matsuoka

Hata

et al. 2023

IJMS

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We aimed to investigate the relationship between mast cell (MC) infiltration into the bladder with urothelial barrier dysfunction and bladder hyperactivity in a chronic bladder ischemia (CBI) rat model. We compared CBI rats (CBI group; n = 10) with normal rats (control group; n = 10). We measured the expression of mast cell tryptase (MCT) and protease-activated receptor 2 (PAR2), which are correlated with C fiber activation via MCT, and Uroplakins (UP Ia, Ib, II and III), which are critical to urothelial barrier function, via Western blotting. The effects of FSLLRY-NH2, a PAR2 antagonist, administered intravenously, on the bladder function of CBI rats were evaluated with a cystometrogram. In the CBI group, the MC number in the bladder was significantly greater (p = 0.03), and the expression of MCT (p = 0.02) and PAR2 (p = 0.02) was significantly increased compared to that of the control group. The 10 μg/kg FSLLRY-NH2 injection significantly increased the micturition interval of CBI rats (p = 0.03). The percentage of UP-II-positive cells on the urothelium with immunohistochemical staining was significantly lower in the CBI group than in the control group (p < 0.01). Chronic ischemia induces urothelial barrier dysfunction via impairing UP II, consequently inducing MC infiltration into the bladder wall and increased PAR2 expression. PAR2 activation by MCT may contribute to bladder hyperactivity.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Involvement of Mast-Cell-Tryptase- and Protease-Activated Receptor 2—Mediated Signaling and Urothelial Barrier Dysfunction with Reduced Uroplakin II Expression in Bladder Hyperactivity Induced by Chronic Bladder Ischemia in the Rat

Akaihata

Matsuoka

Hata

et al. 2023

IJMS

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“…[ 132 ] 2. [ 140 ] Hypericum perforatum Hypericaceae St John’s wort Flowering top Inhibited EFS-induced contractility Rat urinary bladder strips The inhibitory effect on excitatory transmission involve opioid receptors, at least partly [ 27 ] Perilla frutescens Lamiaceae Beefsteak plant Leaf Increased in the micturition interval, improved frequent urination Female SHR rats Improvement of the urothelial presence, anti-inflammatory effects [ 84 ] Peucedanum japonicum Apiaceae Coastal hog fennel Whole plant extract / Isosamidin 1. The extract inhibited agonist-induced rabbit bladder contractile response; decreased micturition frequency 2.…”

Section: Introductionmentioning

confidence: 99%

Medicinal plants and natural products for treating overactive bladder

Chen,

Hoi,

Lee

2024

Chin Med

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Background Overactive bladder (OAB) presents a high prevalence of 16–18% worldwide. The pathophysiology of OAB is still poorly understood while effective therapy or countermeasure are very limited. On the other hand, medicinal plants and herbal remedies have been utilized for treating lower urinary tract symptoms (LUTS) in both Eastern and Western cultures since ancient times. In recent years, accumulating progress has also been made in OAB treatment research by using medicinal plants. Methods Relevant literature on the studies of medicinal plants and herbs used to treat OAB was reviewed. The medicinal plants were summarized and categorized into two groups, single-herb medications and herbal formulations. Results The present review has summarized current understanding of OAB’s pathophysiology, its available treatments and new drug targets. Medicinal plants and natural products which have been used or have shown potential for OAB treatment were updated and comprehensively categorized. Studies on a wide variety of medicinal plants showed promising results, although only a few phytochemicals have been isolated and identified. Until now, none of these herbal compounds have been further developed into clinical therapeutics for OAB. Conclusions This review provides the basis for discovering and designing new phytopharmaceutical candidates with effective and well-tolerated properties to treat OAB. Increasing evidences indicate new strategies with alternative herbal treatment for OAB have high efficacy and safety, showing great promise for their clinical use. Future studies in a rigorously designed controlled manner will be beneficial to further support the eligibility of herbal treatment as OAB therapeutics.

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“…In Chinese medicinals, the stems, leaves, and seeds of P. frutescens are commonly used as effective medicines for treating pain, colds, cough, nausea, poisoning, asthma and constipation [ 19 ]. Modern studies have shown that P. frutescens has anti-bacterial [ 20 ], anti-inflammatory [ 21 – 23 ], anti-cancer [ 24 ], anti-viral [ 25 , 26 ], and anti-aging effects [ 27 ]. Most of these studies have focused on the inhibitory effects of P. frutescens extracts on the common bacteria and plant fungi in daily foods [ 20 , 28 ].…”

Section: Introductionmentioning

confidence: 99%

Integration of transcriptomics and proteomics to elucidate inhibitory effect and mechanism of rosmarinic acid from Perilla frutescens (L.) Britt. in treating Trichophyton mentagrophytes

Guo

Mao

et al. 2023

Chin Med

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Background Dermatophyte caused by Trichophyton mentagrophytes is a global disease with a growing prevalence that is difficult to cure. Perilla frutescens (L.) Britt. is an edible and medicinal plant. Ancient books of Traditional Chinese Medicine and modern pharmacological studies have shown that it has potential anti-fungi activity. This is the first study to explore the inhibitory effects of compounds from P. frutescens on Trichophyton mentagrophytes and its mechanism of action coupled with the antifungal activity in vitro from network pharmacology, transcriptomics and proteomics. Methods Five most potential inhibitory compounds against fungi in P. frutescens was screened with network pharmacology. The antifungal activity of the candidates was detected by a broth microdilution method. Through in vitro antifungal assays screening the compound with efficacy, transcriptomics and proteomics were performed to investigate the pharmacological mechanisms of the effective compound against Trichophyton mentagrophytes. Furthermore, the real-time polymerase chain reaction (PCR) was applied to verify the expression of genes. Results The top five potential antifungal compounds in P. frutescens screened by network pharmacology are: progesterone, luteolin, apigenin, ursolic acid and rosmarinic acid. In vitro antifungal assays showed that rosmarinic acid had a favorable inhibitory effect on fungi. The transcriptomic findings exhibited that the differentially expressed genes of fungus after rosmarinic acid intervention were mainly enriched in the carbon metabolism pathway, while the proteomic findings suggested that rosmarinic acid could inhibit the average growth of Trichophyton mentagrophytes by interfering with the expression of enolase in the glycolysis pathway. Comparison of real-time PCR and transcriptomics results showed that the trends of gene expression in glycolytic, carbon metabolism and glutathione metabolic pathways were identical. The binding modes and interactions between rosmarinic acid and enolase were preliminary explored by molecular docking analysis. Conclusion The key findings of the present study manifested that rosmarinic acid, a medicinal compound extracted from P. frutescens, had pharmacological activity in inhibiting the growth of Trichophyton mentagrophytes by affecting its enolase expression to reduce metabolism. Rosmarinic acid is expected to be an efficacious product for prevention and treatment of dermatophytes.

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Perilla extract improves frequent urination in spontaneously hypertensive rats with enhancement of the urothelial presence and anti‐inflammatory effects

Abstract: The present findings suggest that perilla extract improves frequent urination, and this improvement seems to be mediated, at least in part, by enhancement of the urothelial presence and by the anti-inflammatory effects of perilla.

Cited by 3 publications

References 31 publications

Involvement of Mast-Cell-Tryptase- and Protease-Activated Receptor 2—Mediated Signaling and Urothelial Barrier Dysfunction with Reduced Uroplakin II Expression in Bladder Hyperactivity Induced by Chronic Bladder Ischemia in the Rat

Involvement of Mast-Cell-Tryptase- and Protease-Activated Receptor 2—Mediated Signaling and Urothelial Barrier Dysfunction with Reduced Uroplakin II Expression in Bladder Hyperactivity Induced by Chronic Bladder Ischemia in the Rat

Medicinal plants and natural products for treating overactive bladder

Integration of transcriptomics and proteomics to elucidate inhibitory effect and mechanism of rosmarinic acid from Perilla frutescens (L.) Britt. in treating Trichophyton mentagrophytes

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